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phospho-bIII-Tubulin (Ser-444) Peptide

Scan QR to view Datasheet Catalog #    MBS474023 phospho-bIII-Tubulin (Ser-444) Peptide peptide
Unit / Price
0.05 mg  /  $130 +1 FREE 8GB USB
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 Product Name   

phospho-bIII-Tubulin (Ser-444) Peptide

★Popular Item★
 Also Known As   

phospho-beta-III-Tubulin (Ser-444) Peptide

 Product Synonym Names    phospho-bIII-Tubulin (Ser-444) Peptide
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    Peptide Sequence: Phospho-be ta-III-Tub ulin (Ser-444) synthetic peptide containing amino acid residues around serine 444 of human beta-III-t ubulin. This sequence is not found in beta-I or beta-II-tu bulin isotypes, but is well conserved in beta-III-t ubulins from rat and mouse.
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 Specificity    The peptide is specifically recognized by beta III-Tubulin (Cterminus) antibody (TP1691) in ELISA, and has been shown to block the reactivity of TP1691 in Western blot and immunocytochemistry.
 Form/Format    Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
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 Preparation and Storage    Store at -20 degree C. Stable for 1 year.
 Other Notes    Small volumes of phospho-bIII-Tubulin (Ser-444) Peptide peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for phospho-bIII-Tubulin (Ser-444) Peptide peptide

   Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of alpha/beta-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in beta-tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of betaIII-tubulin in vitro. Unphosphorylated Ser-444 in beta-III-tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in beta-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.
 Application Notes for phospho-bIII-Tubulin (Ser-444) Peptide peptide    Blocking: 1:1,000
ELISA: 50 ng/well
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Product References and Citations for phospho-bIII-Tubulin (Ser-444) Peptide peptide

   • Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041
• Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
• Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
• Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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