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ErbB 2 positive control :: Phospho-ErbB 2 Positive Control

Scan QR to view Datasheet Catalog #    MBS543122
Test Data
Unit / Price
5 Applications  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phospho-ErbB 2, Positive Control

★Popular Item★
 Also Known As   

Phospho-ErbB 2 Positive Control

 Product Synonym Names    Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; c erb B2/neu protein; CD340; CerbB2; Erb B2; Erythroblastosis Virus; HER 2/neu; Herstatin; Metastatic lymph node gene 19 protein; MLN19; NEU; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; TKR1; Tyrosine kinase-type cell surface receptor HER2; v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian); verbb2 antibody
 Product Gene Name   

ErbB 2 positive control

[Similar Products]
 Matching Pairs    Antibody: Phospho-ErbB 2 (MBS543808)
Positive Control: Phospho-ErbB 2 (MBS543122)
 Matching Pairs    Antibody: Phospho-ErbB 2 (MBS543809)
Positive Control: Phospho-ErbB 2 (MBS543122)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    137215
 3D Structure    ModBase 3D Structure for P04626
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 Form/Format    Western Blot Positive Control in SDS sam Ie buffer
 Concentration    Empirically set for optimal signal-to-noise ratio (lot specific)
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 Immunogen    Purified Protein
 Expression    Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
 Molecular Function    Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase
 Structure    Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).
 Subcellular Location    Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm;perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.
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 Preparation and Storage    -20 degree C for long term storage
 Other Notes    Small volumes of ErbB 2 positive control vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ErbB 2 positive control

   Description:
Western Blot Positive Control for Phospho Receptor tyrosine-protein kinase erB-2 (PHER2)

Overview:
The tyrosine kinase receptor erbB2, also known in humans as HER2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). HER2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. These HER receptors determine the regulation of cell growth and differentiation in a complex manner. HER family receptors form homo- or hetero- dimers with other compounds. The activation of these homoheterodimmeric complexes by ligand binding leads to activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. There are at least two humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the HER2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SOS-PAGE gels. HER2 is over expressed in cells of many cancer types. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. HER 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain. The Phospho-Her2 antibody (MBS543807) is made against the phospho-Tyrosine containing peptide (aa 1127-1138) from rat HER2 protein.
The PHER2-selective antibodies were generated against unique antigenic sequences form HER2 protein that is not found in any other protein in the gene bank. The Phospho-HER2 antibodies were affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
 Applications Tested/Suitable for ErbB 2 positive control   

Western Blot (WB)

 Application Notes for ErbB 2 positive control    Western Blot: 1:500
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 Test Data of ErbB 2 positive control    Immunoprecipitation and Western Blot of Herceptin-2 with (MBS# "Please inquire" and MBS541803. Lane 1,2) IP by MBS541803 and WB with MBS# "Please inquire". Lane 3,4) IP by MBS541803 and WB by MBS541803. Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.
ErbB 2 positive control Test Data image
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NCBI/Uniprot data below describe general gene information for ErbB 2. It may not necessarily be applicable to this product.
 NCBI GI #    306840
 NCBI GeneID    2064
 NCBI Accession #    AAA75493.1 [Other Products]
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 UniProt Primary Accession #    P04626 [Other Products]
 UniProt Secondary Accession #    Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5 [Other Products]
 UniProt Related Accession #    P04626 [Other Products]
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 NCBI Official Full Name    HER2 receptor
 NCBI Official Synonym Full Names    erb-b2 receptor tyrosine kinase 2
 NCBI Official Symbol    ERBB2 [Similar Products]
 NCBI Official Synonym Symbols   
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
[Similar Products]
 NCBI Protein Information    receptor tyrosine-protein kinase erbB-2
 UniProt Protein Name    Receptor tyrosine-protein kinase erbB-2
 UniProt Synonym Protein Names   
Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
 UniProt Gene Name    ERBB2 [Similar Products]
 UniProt Synonym Gene Names    HER2; MLN19; NEU; NGL; MLN 19 [Similar Products]
 UniProt Entry Name    ERBB2_HUMAN
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 NCBI Summary for ErbB 2    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ErbB 2    HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Oncoprotein; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; integral to membrane; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex

Molecular Function: ATP binding; ErbB-3 class receptor binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein C-terminus binding; protein dimerization activity; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell proliferation; cell surface receptor linked signal transduction; enzyme linked receptor protein signaling pathway; heart development; MAPKKK cascade; motor axon guidance; myelination; negative regulation of immature T cell proliferation in the thymus; neuromuscular junction development; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of angiogenesis; regulation of microtubule-based process; regulation of phosphoinositide 3-kinase cascade; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing

Disease: Gastric Cancer; Glioma Susceptibility 1; Lung Cancer
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 Research Articles on ErbB 2    1. Estrogen receptor, progesterone receptor or human epidermal growth factor receptor 2 samples with high-methylation risk score had the worst 5-year survival rate and overall survival time.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ErbB 2 positive controlPathways associated with ErbB 2 positive control
 Reference Product  PubMed Publications
 EGFR positive control  >3468 publications with ErbB 2 and EGFR
 ERBB3 positive control  >957 publications with ErbB 2 and ERBB3
 SRC positive control  >469 publications with ErbB 2 and SRC
 CTNNB1 positive control  >218 publications with ErbB 2 and CTNNB1
 GRB2 positive control  >111 publications with ErbB 2 and GRB2
 ERBB2IP positive control  >50 publications with ErbB 2 and ERBB2IP
 SHC1 positive control  >37 publications with ErbB 2 and SHC1
 PTPN11 positive control  >16 publications with ErbB 2 and PTPN11
 UBC positive control  >7 publications with ErbB 2 and UBC
 HSP90AA1 positive control  >3 publications with ErbB 2 and HSP90AA1
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
Diseases associated with ErbB 2 positive controlOrgans/Tissues associated with ErbB 2 positive control
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >14082 publications with ErbB 2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >3935 publications with ErbB 2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >3306 publications with ErbB 2 and Neoplasm Metastasis
 Recurrence Antibodies  >1986 publications with ErbB 2 and Recurrence
 Disease Progression Antibodies  >1878 publications with ErbB 2 and Disease Progression
 Neoplasms, Experimental Antibodies  >1839 publications with ErbB 2 and Neoplasms, Experimental
 Gastrointestinal Neoplasms Antibodies  >1542 publications with ErbB 2 and Gastrointestinal Neoplasms
 Lymphatic Metastasis Antibodies  >1496 publications with ErbB 2 and Lymphatic Metastasis
 Carcinogenesis Antibodies  >1423 publications with ErbB 2 and Carcinogenesis
 Lung Neoplasms Antibodies  >1351 publications with ErbB 2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2441 publications with ErbB 2 and Blood
 Lymph Node Antibodies  >2200 publications with ErbB 2 and Lymph Node
 Lung Antibodies  >1592 publications with ErbB 2 and Lung
 Stomach Antibodies  >935 publications with ErbB 2 and Stomach
 Brain Antibodies  >760 publications with ErbB 2 and Brain
 Liver Antibodies  >677 publications with ErbB 2 and Liver
 Bone Antibodies  >534 publications with ErbB 2 and Bone
 Prostate Antibodies  >531 publications with ErbB 2 and Prostate
 Mammary Gland Antibodies  >500 publications with ErbB 2 and Mammary Gland
 Heart Antibodies  >489 publications with ErbB 2 and Heart
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