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ELANE protease :: Neutrophil Elastase, Lyophilized Protease

Scan QR to view Datasheet Catalog #    MBS135624 ELANE protease
Unit / Price
0.5 mg  /  $630 +1 FREE 8GB USB
1 mg  /  $1,085 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neutrophil Elastase, Lyophilized (ELANE), Protease

★Popular Item★
 Also Known As   

Human Neutrophil Elastase, Lyophilized

 Product Gene Name   

ELANE protease

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 19; NC_000019.9 (852291..856246). Location: 19p13.3
 OMIM    130130
 3D Structure    ModBase 3D Structure for P08246
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 Purity/Purification    >95% by SDS-PAGE analysis
 Form/Format    Lyophilized powder
 Concentration    lyophilized (lot specific)
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 Source    Human neutrophil
 Specific Activity    875 U/mg on Suc-Ala-Ala-Ala-pNA
 Buffer    Salt free
 Extinction Coefficient    0.985
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 Preparation and Storage    Store at 4 degree C. Shelf Life: 12 months from delivery
 Other Notes    Small volumes of ELANE protease vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ELANE protease

   Chromatographically purified from leucocytes of purulent human sputum and lyophilized. The lyophilized, salt-free, soluble powder is stable for over a year when stored at 5 C. The protein is greater than 95percent pure by SDS-PAGE and is free of cathepsin G, MPO and lysozyme. 800-900 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. 15000-19000 units per mg protein on the substrate MeO-Suc-Ala-Ala-Pro-Val-pNA. One unit will hydrolyze 1 nanomole of substrate per minute at pH 7.5 and 25 C. The following diluents and concentrations are recommended: 1) 1 mg/ml in 0.05 M Na Acetate pH 5.0 containing 0.1 M NaCl. Stable 7 days at 5 C (in ice). 2) 10 mg/ml in 50percent glycerol 50percent 0.02 M Na Acetate pH 5. Stable at least 60 days at -20 C with 20 cycles between -20 C and 5 C. 3) May be dissolved at 10 mg/ml in 2 mM HoAc and re-lyophilized in smaller quantities. Stability is better in acidic (pH 5.0) than basic buffers. Inactivation may occur at pH 8.0 due to autolysis and/or proteolysis by trypsin such as bovine pancreatic trypsin. Proteolysis can be inhibited by benzamidine. Autolysis and proteolysis can be inhibited by elastatinal. However, elastatinal is also an inhibitor of elastase. Low pH protects the elastase from both autolysis and proteolysis.
 Product Categories/Family for ELANE protease    Elastase, Proteases; Human Neutrophil Elastase
NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.
 NCBI GI #    4503549
 NCBI GeneID    1991
 NCBI Accession #    NP_001963.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001972.2 [Other Products]
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 UniProt Primary Accession #    P08246 [Other Products]
 UniProt Secondary Accession #    P09649; Q6B0D9; Q6LDP5 [Other Products]
 UniProt Related Accession #    P08246 [Other Products]
 Molecular Weight    29,500
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 NCBI Official Full Name    neutrophil elastase preproprotein
 NCBI Official Synonym Full Names    elastase, neutrophil expressed
 NCBI Official Symbol    ELANE [Similar Products]
 NCBI Official Synonym Symbols   
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
[Similar Products]
 NCBI Protein Information    neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
 UniProt Protein Name    Neutrophil elastase
 UniProt Synonym Protein Names   
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
 Protein Family    Neutrophil elastase
 UniProt Gene Name    ELANE [Similar Products]
 UniProt Synonym Gene Names    ELA2; HLE [Similar Products]
 UniProt Entry Name    ELNE_HUMAN
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 NCBI Summary for ELANE    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
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 UniProt Comments for ELANE    ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Motility/polarity/chemotaxis; EC 3.4.21.37; Cell cycle regulation; Protease; Cell surface

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule

Molecular Function: heparin binding; peptidase activity; protein binding; protease binding; serine-type endopeptidase activity; cytokine binding; endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of immune response; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; negative regulation of chemotaxis; proteolysis; phagocytosis; cellular calcium ion homeostasis; extracellular matrix disassembly; collagen catabolic process; positive regulation of MAP kinase activity; negative regulation of inflammatory response; defense response to bacterium; protein catabolic process; response to yeast; leukocyte migration; negative regulation of chemokine biosynthetic process; acute inflammatory response to antigenic stimulus; response to UV

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant
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 Research Articles on ELANE    1. Chamomile infusion inhibited expression of neutrophil elastase and matrix metalloprotease-9 in adenocarcinoma gastric cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ELANE proteasePathways associated with ELANE protease
 Reference Product  PubMed Publications
 CSF3R protease  >7 publications with ELANE and CSF3R
 CSF3 protease  >1 publications with ELANE and CSF3
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with ELANE proteaseOrgans/Tissues associated with ELANE protease
 Disease Name  Pubmed Publications
 Neutropenia Antibodies  >31 publications with ELANE and Neutropenia
 Cyclic neutropenia Antibodies  >7 publications with ELANE and Cyclic neutropenia
 NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT Antibodies  >5 publications with ELANE and NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT
 Lung Diseases Antibodies  >3 publications with ELANE and Lung Diseases
 Shock Antibodies  >1 publications with ELANE and Shock
 Liver Diseases Antibodies  >1 publications with ELANE and Liver Diseases
 Disease Models, Animal Antibodies  >1 publications with ELANE and Disease Models, Animal
 Pain Antibodies  >1 publications with ELANE and Pain
 Weight Loss Antibodies  >1 publications with ELANE and Weight Loss
 Inflammation Antibodies  >1 publications with ELANE and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with ELANE and Blood
 Bone Marrow Antibodies  >5 publications with ELANE and Bone Marrow
 Lung Antibodies  >3 publications with ELANE and Lung
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