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| Product Name
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Alanine Aminotransferase (GPT), Protein ★Popular Item★
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| Also Known As
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Alanine Aminotransferase (ALT, GPT), Human Liver
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| Product Synonym Names
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Glutamate Pyruvate Transaminase; Alanine Transaminase; serum glutamic-pyruvic transaminase; SGPT; GPT; ALT; ALAT; Glutamate Pyruvate Transaminase (GPT, ALT), Human Liver - Liquid
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| MBS173088 MSDS
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| OMIM
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138200
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| Specificity
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Liver damage or disease
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| Purity/Purification
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Purified
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| Form/Format
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Suspension in tris-buffered 1.8 M ammonium sulfate, pH 7.5
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| Source
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Human Liver
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| EC Number
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2.6.1.2
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| UNSPSC Code
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51131907
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| Activity
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700 U/mL (Dimension@ Clinical Chemistry System)
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| Unit Definition
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One unit will catalyze the transamination of one micromole of L-alanine to alphaketoglutarate,
forming L-glutamate and pyruvate, per minute at 37°C and pH 7.4.
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| Protein
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24 mg/mL (Coomassie)
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| Specific Acitivty
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30 units/mg protein
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| Appearance
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Yellow-green suspension
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| Contaminants
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ALP: < or = 0.1%
CK: < or = 0.1%
GGT: < or = 0.1%
GOT/AST: 0.6%
LDH: < or = 0.1%
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| Recertification
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1 year
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| Protein
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> 0.5 mg protein/mL (Coomassie)
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| Contaminants
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CPK, ALP & gGT: <=0.1%, AST/GOT: <=3%, LDH: <=1.0%
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| CAS Number
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9000-86-6
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| Activity
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Typically >50 U/mL (Dimension Clinical Chemistry System)
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| Preparation and Storage
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2-8 degree C. Do not freeze
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| Other Notes
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Small volumes of GPT protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for GPT protein
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MyBioSource is the leading producer of Human Liver Glutamate Pyruvate Transaminase/Alanine transaminase (GPT/ALT) for medical research and clinical diagnostic assays for diagnosis of liver disease.
Custom preparations, technical support, bulk quantities and aliquoting available.
Elevated levels of Alanine Transaminase (ALT)often suggest the existence of other medical problems such as alcoholic or viral hepatitis, congestive heart failure, liver damage, biliary duct problems, infectious mononucleosis, or myopathy.
Human GPT/ALT is an enzyme that catalyzes the reversible transfer of an amino group from alanine to a-ketoglutarate. It is found in serum and in various bodily tissues but is most commonly associated with the liver. Also called Serum Glutamate Pyruvate Transaminase (SGPT) or Alanine Aminotransferase (ALAT).
The alanine aminotransferase (ALT) blood test is typically used to detect liver injury, and is often ordered in conjunction with Aspartate Aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease. AST and ALT are considered to be two of the most important tests to detect liver injury, although ALT is more specific than AST. Sometimes AST is compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage.
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| Product Categories/Family for GPT protein
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Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Alanine Aminotransferase (alt; Gpt)
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| NCBI/Uniprot data below describe general gene information for GPT. It may not necessarily be applicable to this product. |
| NCBI GI #
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1763096
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| NCBI GeneID
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2875
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| NCBI Accession #
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AAC51155.1
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[Other Products]
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| UniProt Secondary Accession #
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P78398; Q93076; B0YJ18; D3DWM7
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[Other Products]
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| UniProt Related Accession #
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P24298
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[Other Products]
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| Molecular Weight
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~100 kDa
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[Similar Products]
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| NCBI Official Full Name
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glutamate pyruvate transaminase
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| NCBI Official Synonym Full Names
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glutamic-pyruvate transaminase (alanine aminotransferase)
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| NCBI Official Symbol
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GPT
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[Similar Products]
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| NCBI Official Synonym Symbols
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AAT1; ALT1; GPT1
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[Similar Products]
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| NCBI Protein Information
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alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1
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| UniProt Protein Name
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Alanine aminotransferase 1
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| UniProt Synonym Protein Names
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Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
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| Protein Family
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Alanine aminotransferase
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| UniProt Gene Name
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GPT
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[Similar Products]
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| UniProt Synonym Gene Names
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AAT1; GPT1; ALT1; GPT 1
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[Similar Products]
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| UniProt Entry Name
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ALAT1_HUMAN
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| NCBI Summary for GPT
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This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
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| UniProt Comments for GPT
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GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
Protein type: Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: cytosol
Molecular Function: alanine transaminase activity; pyridoxal phosphate binding
Biological Process: amino acid biosynthetic process; L-alanine catabolic process
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| Research Articles on GPT
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1. Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with GPT protein | Pathways associated with GPT protein |
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| Diseases associated with GPT protein | Organs/Tissues associated with GPT protein |
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