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Apo AI protein :: Apolipoprotein AI (Apo AI) Protein

Scan QR to view Datasheet Catalog #    MBS390131
Testing Data)
Unit / Price
0.5 mg  /  $215 +1 FREE 8GB USB
1 mg  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein AI (Apo AI), Protein

★Popular Item★
 Also Known As   

Ultra Pure Human Apolipoprotein AI (Apo AI)

 Product Synonym Names    Ultra Pure Human Apolipoprotein AI (Apo AI)
 Product Gene Name   

Apo AI protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 11; NC_000011.9 (116706467..116708338, complement). Location: 11q23-q24
 OMIM    105200
 3D Structure    ModBase 3D Structure for P02647
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 Purity/Purification    After series ultracentrifugations, High Density Lipoprotein (HDL) is isolated from human plasma. Apo AI is purified from delipidated HDL, followed by gel-filtration.
Purity: > 99 % by SDS-PAGE
 Concentration    1mg / ml, determined by the Lowry method (lot specific)
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 Source Note    From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
 Buffer    20 mM Tris-HCl, 0.14 M NaCl, 0.02 % NaN3, 0.5 mM EDTA, pH 8.0.
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 Preparation and Storage    -20 degree C for long-term storage, 2-8 degree C for short-term storage. Aliquot to avoid repeated freezing and thawing.
 Other Notes    Small volumes of Apo AI protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for Apo AI protein    Human Aplipoproteins
 Applications Tested/Suitable for Apo AI protein   

SDS-PAGE

 Application Notes for Apo AI protein    Purity > 99% by SDS-PAGE.
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 Testing Data) of Apo AI protein    Apo AI protein Testing Data) image
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NCBI/Uniprot data below describe general gene information for Apo AI. It may not necessarily be applicable to this product.
 NCBI GI #    296635
 NCBI GeneID    335
 NCBI Accession #    CAA25519.1 [Other Products]
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 UniProt Primary Accession #    P02647 [Other Products]
 UniProt Secondary Accession #    Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866 [Other Products]
 UniProt Related Accession #    P02647 [Other Products]
 Molecular Weight    30,778 Da
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 NCBI Official Full Name    apolipoprotein AI
 NCBI Official Synonym Full Names    apolipoprotein A-I
 NCBI Official Symbol    APOA1 [Similar Products]
 NCBI Protein Information    apolipoprotein A-I; apo-AI
 UniProt Protein Name    Apolipoprotein A-I
 UniProt Synonym Protein Names   
Apolipoprotein A1Cleaved into the following chain:Truncated apolipoprotein A-I; Alternative name(s):; Apolipoprotein A-I(1-242)
 UniProt Gene Name    APOA1 [Similar Products]
 UniProt Synonym Gene Names    Apo-AI; ApoA-I [Similar Products]
 UniProt Entry Name    APOA1_HUMAN
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 NCBI Summary for Apo AI    This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Apo AI    Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Ref.20 Ref.25 Ref.31 Ref.34

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Ref.32

Post-translational modification: Palmitoylated. Ref.28Met-110 and Met-136 are oxidized to methionine sulfoxides.Phosphorylation sites are present in the extracellular medium.

Polymorphism: Genetic variations in APOA1 can result in APOA1 deficiency and are associated with low levels of HDL cholesterol [

MIM:107680].

Involvement in disease: High density lipoprotein deficiency 2 (HDLD2) [MIM:604091]: Inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39High density lipoprotein deficiency 1 (HDLD1) [MIM:205400]: Recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (Ref.44 and Ref.45). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Ref.38 Ref.39Amyloidosis 8 (AMYL8) [MIM:105200]: A hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39 Ref.44 Ref.45 Ref.47 Ref.52

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.

Mass spectrometry: Molecular mass is 28081 Da from positions 25 - 267. Determined by ESI. Without methionine sulfoxide. Ref.32Molecular mass is 28098 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-110. Ref.32Molecular mass is 28095 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-136. Ref.32Molecular mass is 28114 Da from positions 25 - 267. Determined by ESI. With 2 methionine sulfoxides, oxidation at Met-110 and Met-136. Ref.32
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 Research Articles on Apo AI    1. Data indicate that the larger particles generated in BHK-ABCA1 cells with apoAI at the low available cell lipid:apoAI ratio had no more than 3 apoAI molecules per lipoprotein particle.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Apo AI proteinPathways associated with Apo AI protein
 Reference Product  PubMed Publications
 APOB protein  >418 publications with Apo AI and APOB
 ABCA1 protein  >105 publications with Apo AI and ABCA1
 APOC3 protein  >58 publications with Apo AI and APOC3
 CETP protein  >46 publications with Apo AI and CETP
 LCAT protein  >36 publications with Apo AI and LCAT
 APOA2 protein  >33 publications with Apo AI and APOA2
 ALB protein  >7 publications with Apo AI and ALB
 TTR protein  >5 publications with Apo AI and TTR
 MTTP protein  >1 publications with Apo AI and MTTP
 Products by Pathway  Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 ABCA Transporters In Lipid Homeostasis Pathway antibodies  ABCA Transporters In Lipid Homeostasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Diseases associated with Apo AI proteinOrgans/Tissues associated with Apo AI protein
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >382 publications with Apo AI and Cardiovascular Diseases
 Atherosclerosis Antibodies  >232 publications with Apo AI and Atherosclerosis
 Dyslipidemias Antibodies  >163 publications with Apo AI and Dyslipidemias
 Heart Diseases Antibodies  >150 publications with Apo AI and Heart Diseases
 Hyperlipidemias Antibodies  >117 publications with Apo AI and Hyperlipidemias
 Insulin Resistance Antibodies  >81 publications with Apo AI and Insulin Resistance
 Inflammation Antibodies  >79 publications with Apo AI and Inflammation
 Hypertension Antibodies  >66 publications with Apo AI and Hypertension
 Hypercholesterolemia Antibodies  >57 publications with Apo AI and Hypercholesterolemia
 Hypertriglyceridemia Antibodies  >55 publications with Apo AI and Hypertriglyceridemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >670 publications with Apo AI and Blood
 Liver Antibodies  >141 publications with Apo AI and Liver
 Heart Antibodies  >133 publications with Apo AI and Heart
 Muscle Antibodies  >30 publications with Apo AI and Muscle
 Brain Antibodies  >25 publications with Apo AI and Brain
 Ovary Antibodies  >13 publications with Apo AI and Ovary
 Lung Antibodies  >10 publications with Apo AI and Lung
 Intestine Antibodies  >9 publications with Apo AI and Intestine
 Pancreas Antibodies  >6 publications with Apo AI and Pancreas
 Embryonic Tissue Antibodies  >3 publications with Apo AI and Embryonic Tissue
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