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PYCARD protein :: Apoptosis-associated speck-like protein containing a CARD Protein

Scan QR to view Datasheet Catalog #    MBS2889532
Testing Data(TD)
Unit / Price
0.1 mg  /  $525 +1 FREE 8GB USB
0.2 mg  /  $795 +1 FREE 8GB USB
0.5 mg  /  $1,365 +1 FREE 8GB USB
1 mg  /  $1,455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apoptosis-associated speck-like protein containing a CARD (PYCARD), Protein

★Popular Item★
 Also Known As   

Apoptosis-associated speck-like protein containing a CARD

 Product Synonym Names    ASC; CARD5; TMS1; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
 Product Gene Name   

PYCARD protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-195aa
 Sequence    MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYYGK VLTDEQYQAV RAEPTNPSKM RKLFSFTPAW NWTTCKDLLL QALRESQSYL VEDLERS
 OMIM    606838
 3D Structure    ModBase 3D Structure for Q9ULZ3
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 Host    E Coli
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 Purity/Purification    >90%
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Organism    Homo sapiens(human)
 Buffer    50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
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 Preparation and Storage    Store at -20C for 6 months.
Avoid repeated freezing and thawing.
 Other Notes    Small volumes of PYCARD protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PYCARD protein

   Recombinant protein with His-tag.
 Applications Tested/Suitable for PYCARD protein   

ELISA (EIA), Western Blot (WB), Affinity Purification (AP)

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 Testing Data(TD) of PYCARD protein    PYCARD protein Testing Data(TD) image
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NCBI/Uniprot data below describe general gene information for PYCARD. It may not necessarily be applicable to this product.
 NCBI GI #    10835256
 NCBI GeneID    29108
 NCBI Accession #    NP_037390.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013258.4 [Other Products]
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 UniProt Primary Accession #    Q9ULZ3 [Other Products]
 UniProt Secondary Accession #    Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8 [Other Products]
 UniProt Related Accession #    Q9ULZ3 [Other Products]
 Molecular Weight    21.45 kD
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 NCBI Official Full Name    apoptosis-associated speck-like protein containing a CARD isoform a
 NCBI Official Synonym Full Names    PYD and CARD domain containing
 NCBI Official Symbol    PYCARD [Similar Products]
 NCBI Official Synonym Symbols   
ASC; TMS; TMS1; CARD5; TMS-1
[Similar Products]
 NCBI Protein Information    apoptosis-associated speck-like protein containing a CARD
 UniProt Protein Name    Apoptosis-associated speck-like protein containing a CARD
 UniProt Synonym Protein Names   
Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
 Protein Family    Apoptosis-associated speck-like protein
 UniProt Gene Name    PYCARD [Similar Products]
 UniProt Synonym Gene Names    ASC; CARD5; TMS1; hASC [Similar Products]
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 NCBI Summary for PYCARD    This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PYCARD    Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590).
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 Research Articles on PYCARD    1. ASC specks released by microglia bind to amyloid-beta and increase amyloid-beta oligomer and aggregate formation, acting as an inflammation-driven cross-seed for amyloid-beta pathology
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PYCARD proteinPathways associated with PYCARD protein
 Reference Product  PubMed Publications
 CASP1 protein  >224 publications with PYCARD and CASP1
 NLRP3 protein  >159 publications with PYCARD and NLRP3
 NLRC4 protein  >27 publications with PYCARD and NLRC4
 AIM2 protein  >24 publications with PYCARD and AIM2
 NLRP1 protein  >14 publications with PYCARD and NLRP1
 IL1B protein  >11 publications with PYCARD and IL1B
 MEFV protein  >8 publications with PYCARD and MEFV
 CASP5 protein  >5 publications with PYCARD and CASP5
 TP53 protein  >4 publications with PYCARD and TP53
 PSTPIP1 protein  >2 publications with PYCARD and PSTPIP1
 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
Diseases associated with PYCARD proteinOrgans/Tissues associated with PYCARD protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >147 publications with PYCARD and Inflammation
 Necrosis Antibodies  >56 publications with PYCARD and Necrosis
 Disease Models, Animal Antibodies  >26 publications with PYCARD and Disease Models, Animal
 Nervous System Diseases Antibodies  >17 publications with PYCARD and Nervous System Diseases
 Brain Diseases Antibodies  >16 publications with PYCARD and Brain Diseases
 Lung Diseases Antibodies  >15 publications with PYCARD and Lung Diseases
 Cardiovascular Diseases Antibodies  >15 publications with PYCARD and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >14 publications with PYCARD and Adenocarcinoma
 Kidney Diseases Antibodies  >12 publications with PYCARD and Kidney Diseases
 Atherosclerosis Antibodies  >10 publications with PYCARD and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >85 publications with PYCARD and Blood
 Bone Antibodies  >42 publications with PYCARD and Bone
 Brain Antibodies  >25 publications with PYCARD and Brain
 Kidney Antibodies  >23 publications with PYCARD and Kidney
 Lung Antibodies  >23 publications with PYCARD and Lung
 Liver Antibodies  >19 publications with PYCARD and Liver
 Vascular Antibodies  >13 publications with PYCARD and Vascular
 Intestine Antibodies  >12 publications with PYCARD and Intestine
 Skin Antibodies  >10 publications with PYCARD and Skin
 Placenta Antibodies  >4 publications with PYCARD and Placenta
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