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CD274 protein

Scan QR to view Datasheet Catalog #    MBS666818 CD274 protein
Unit / Price
0.025 mg  /  $575 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD274, Protein

★Popular Item★
 Also Known As   

CD274 (B7-H1)-muIg-Biotin

 Product Synonym Names    Human CD274(B7-H1)-muIg/Biotin Fusion Protein
 Product Gene Name   

CD274 protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MuCD8 alpha signal peptide residual amino acids + linker:(1) kpqapelrgs as

CD274 mature EC: (224aa): (13)ftvtvp kdlyvveygs nmtieckfpv ekqldlaali vywemedkni iqfvhgeedl kvqhssyrqr arllkdqlsl gnaalqitdv klqdagvyrc misyggadyk ritvkvnapy nkinqrilvv dvtseheltc qaegypkaev iwtssdhqvl sgkttttnsk reeklfnvts tlrintttne ifyctfrrld peenhtaelv ipelplahpp nerthtr

Linke r + Murine IgG2a Hinge + Fc (235aa):(237)gte prgptikpcp pckcpapnll ggpsvfifpp kikdvlmisl spivtcvvvd vseddpdvqi swfvnnvevh taqtqthred ynstlrvvsa lpiqhqdwms gkefkckvnn kdlpapiert iskpgsvrap qvyvlpppee emtkkqvtlt cmvtdfmped iyvewtnngk telnykntep vldsdgsyfm ysklrvekkn wvernsyscs vvheglhnhh ttksfsrtpg (471)
 OMIM    605402
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 Host    CHO cells
 Species Reactivity    Human
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 Form/Format    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
 Concentration    0.5 mg/ml (lot specific)
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 Molecular Structure    A soluble fusion protein consisting of the murine CD8 alpha leader sequence, the mature extracellular (224aa) domain of human CD274 fused to murine IgG2a Fc + hinge (233aa).
 Transfectant Cell Line    CHO
 Performance    Purity of recombinant protein was >90% by SDS-PAGE. N-terminal sequence was as predicted: KPQAP. Biotinylated recombinant protein was active in EIA, binding to anti-CD274 mAbs ANC6H1, clone M1H1, and recombinant CD279(PD1).

Predicted monomeric (non glycosylated) molecular weight: 54.4kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
 Conjugation    Biotin
 Production    Human CD27-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
 Buffer    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
 Molecular Weight    Predicted monomeric (non glycosylated) molecular weight: 54.4kd.
The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
 Buffer    50mM Sodium Phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
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 Preparation and Storage    Store at 2 - 5 degree C. Freeze/Thawing is not recommended
Product should retain activity for at least 6 months after shipping date when stored as recommended.
 Other Notes    Small volumes of CD274 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CD274 protein

   Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection (3). CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma are associated with poor prognosis (1). Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival (2). Gamma Interferon and PHA can up regulate CD274 expression on T cells.
 Applications Tested/Suitable for CD274 protein   

ELISA (EIA)

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NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.
 NCBI GI #    112363645
 NCBI GeneID    29126
 NCBI Accession #    ABI16084.1 [Other Products]
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 UniProt Secondary Accession #    Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27 [Other Products]
 UniProt Related Accession #    Q9NZQ7 [Other Products]
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 NCBI Official Full Name    CD274, partial
 NCBI Official Synonym Full Names    CD274 molecule
 NCBI Official Symbol    CD274 [Similar Products]
 NCBI Official Synonym Symbols   
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
[Similar Products]
 NCBI Protein Information    programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
 UniProt Protein Name    Programmed cell death 1 ligand 1
 UniProt Synonym Protein Names   
B7 homolog 1; B7-H1; CD_antigen: CD274
 Protein Family    Programmed cell death
 UniProt Gene Name    CD274 [Similar Products]
 UniProt Synonym Gene Names    B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1 [Similar Products]
 UniProt Entry Name    PD1L1_HUMAN
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 UniProt Comments for CD274    CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction
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Product References and Citations for CD274 protein

   1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.
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 Research Articles on CD274    1. PD-L1 expression on myeloid precursor cells is increased quantitatively and qualitatively in AML patients after gamma-interferon treatment initiation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD274 proteinPathways associated with CD274 protein
 Reference Product  PubMed Publications
 CD80 protein  >390 publications with CD274 and CD80
 PDCD1 protein  >322 publications with CD274 and PDCD1
 STAT1 protein  >11 publications with CD274 and STAT1
 PTEN protein  >8 publications with CD274 and PTEN
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram
Diseases associated with CD274 proteinOrgans/Tissues associated with CD274 protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >106 publications with CD274 and Inflammation
 Liver Diseases Antibodies  >84 publications with CD274 and Liver Diseases
 Necrosis Antibodies  >73 publications with CD274 and Necrosis
 Nervous System Diseases Antibodies  >69 publications with CD274 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >65 publications with CD274 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >59 publications with CD274 and Adenocarcinoma
 Lung Diseases Antibodies  >44 publications with CD274 and Lung Diseases
 Kidney Diseases Antibodies  >43 publications with CD274 and Kidney Diseases
 Lung Neoplasms Antibodies  >40 publications with CD274 and Lung Neoplasms
 Recurrence Antibodies  >26 publications with CD274 and Recurrence
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >755 publications with CD274 and Blood
 Lymph Node Antibodies  >78 publications with CD274 and Lymph Node
 Lung Antibodies  >70 publications with CD274 and Lung
 Bone Antibodies  >58 publications with CD274 and Bone
 Skin Antibodies  >45 publications with CD274 and Skin
 Brain Antibodies  >31 publications with CD274 and Brain
 Pancreas Antibodies  >28 publications with CD274 and Pancreas
 Placenta Antibodies  >19 publications with CD274 and Placenta
 Stomach Antibodies  >13 publications with CD274 and Stomach
 Mouth Antibodies  >9 publications with CD274 and Mouth
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