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CD278 protein

Scan QR to view Datasheet Catalog #    MBS666804
Testing Data
Unit / Price
0.025 mg  /  $575 +1 FREE 8GB USB
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 Product Name   

CD278, Protein

★Popular Item★
 Also Known As   


 Product Synonym Names    Human CD278(ICOS)-muIg/Biotin Fusion Protein
 Product Gene Name   

CD278 protein

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 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    CHO cells
 Species Reactivity    Human
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 Form/Format    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA,0.04 NaN3 as a preservative).
 Concentration    0.5 mg/ml (lot specific)
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 Molecular Structure    A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8 kd (amino acid composition only).
 Transfectant Cell Line    CHO
 Performance    CD278(ICOS)-muIg/Biotin was tested for ability to detect Goat-anti-Mouse-captured CD275(ICOSL)-muIg in EIA utilizing Streptavidin/HRP detection and TMB/H2 O2 substrate chromagen.
 PERFORMANCE    CD278(ICOS)-mulg/Biotin was tested for its ability to bind to cell surface CD275(ICOSL) in FACS. Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul 1 of ICOS-mulg/Biotin at a concentration of 5 )Ug/ml. Cells were washed twice and incubated with 2 reagent Streptavidin/R-Phycoerythrin (Catalog #MBS666773), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.39 log 10 fluorescent units when compared to a recombinant mulgFc Control Protein/Biotin negative control (Catalog #MBS666830). Binding was blocked when reagent was pre incubated 10 minutes with a 10 fold excess of CD275(GL50,ICOSL)-mulg fusion protein (catalog #MBS666861).
 Conjugation    Biotin
 Production    Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using size exclusion chromatography.), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
 Buffer    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
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 Preparation and Storage    Store at 2 - 5C. Freeze/Thawing is not recommended.
 Other Notes    Small volumes of CD278 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CD278 protein

   Information: The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Each secondary signal from CD28 or ICOS results in a discrete cytokine secretion profile displayed by the activated T cell.1 Both activation processes are effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be a ligand for ICOS.3 ICOS-muIg recombinant protein binds to recombinant GL50-muIg in EIA.
 Applications Tested/Suitable for CD278 protein   

Flow Cytometry (FC/FACS), ELISA (EIA)

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 Testing Data of CD278 protein    CD278 protein Testing Data image
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Product References and Citations for CD278 protein

   Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2 Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3 Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4 Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308. 5 A.J. McAdam, et al, (2000) J Immunol 165: 5035-5040.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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