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Histones Protein

Scan QR to view Datasheet Catalog #    MBS634742 Histones protein
Unit / Price
250 mg  /  $300 +1 FREE 8GB USB
1 g  /  $390 +1 FREE 8GB USB
 
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 Product Name   

Histones, Protein

★Popular Item★
 Also Known As   

Histones

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    Calf Thymus
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 Purity/Purification    Purified
Purified (> 90% pure). Crude blend of extracted histones that are unfractionated and unpurified. May contain numerous subfractions due to acetylation, methylation and phosphorylation of various amino acid residues.
 Form/Format    Supplied as an ethanol dried powder.
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 CAS Number    37244-51-2
 Appearance    White to off white powder.
 Solubility    Soluble in PBS or water soluble, pH7.0
 Quality Control    SDS-PAGE
 A260(mg/ml)    0.178
 A280(mg/ml)    0.288
 A280/A260    1.162
 Reconstitution    Reconstitute with sterile physiological saline, PBS, pH 7.1, or water when pH is adjusted to neutral.
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 Preparation and Storage    4 degree C
 Other Notes    Small volumes of Histones protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Histones protein

   Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. Histones are a group of similar, small, highly conserved nuclear proteins that bind to DNA by their many basic residues. The presence of autoantibodies to histones are frequently found in several rheumatic disorders (1). In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3 (2). The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity (3).

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limited number of types of fractions (see below) with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism.
Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.
With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature.
The nucleoprotein complex of histone and deoxyribonucleic acid is referred to as nucleohistone or deoxyribonucleoprotein. It is important as a source of its two components as well as an entity in itself for physical studies. Intracellularly, these complexes may be important factors in chromosomal structure and gene transcription. Kornberg (1974) has proposed a model for chromatin in which 200 DNA base pairs are coiled on the outside of a histone unit composed of (H3)2(H4)2 tetramer and two each of H2a and H2b.
 Product Categories/Family for Histones protein    Molecular Biology; MB-Histones
NCBI/Uniprot data below describe general gene information for Histones. It may not necessarily be applicable to this product.
 NCBI GI #    510880419
 NCBI Accession #    YP_008053087.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_021313.1 [Other Products]
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 NCBI Official Full Name    histones H3 and H4
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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