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Protein A, S. aureus, Cowan Strain Reagent

Scan QR to view Datasheet Catalog #    MBS635420 Protein A, S. aureus, Cowan Strain reagent
Unit / Price
1 mg  /  $410 +1 FREE 8GB USB
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 Product Name   

Protein A, S. aureus, Cowan Strain, Reagent

★Popular Item★
 Also Known As   

Protein A, S. aureus, Cowan Strain (Biotin)

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Species Reactivity    Human IgG: 100%
Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50%
Rat IgG: 25%
Chicken and Hamster IgG: 1%
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 Form/Format    Supplied as a powder in 10mM HEPES, pH 8, 0.15M sodium chloride. Labeled with Biotin. Reconstitute in 500ul of sterile ddH2O, 0.1% BSA.
 Concentration    ~2mg/ml (after reconstitution) (lot specific)
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 IgG Binding Activity: 92%   
 Protein A Biotinlylated    99%
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of Protein A, S. aureus, Cowan Strain reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Protein A, S. aureus, Cowan Strain reagent

   Protein A, isolated from a special strain of Staphylococcus aureus, is a 41,000D protein capable of binding to the Fc portion of immunoglobulin. This interaction is not species specific, as Protein A has been shown to bind IgG from a variety of species. The binding was thought to be restricted to IgG, but subsequent studies have demonstrated that Protein A can interact with IgA, IgM and subclasses of IgG from different species. Biotinylated Protein A is a useful reagent for detecting or localizing immunoglobulins on cell surfaces or in tissue sections. Used with the appropriate Avidin D conjugate, immunoglobulins can be studied at the fluorescent or electron microscopic level. Biotinylated Protein A can also be employed for the detection of positive clones in monoclonal antibody production by using Avidin D conjugated with peroxidase or other marker enzymes. Used in many systems as a substitute for a biotinylated second antibody. Because Biotinylated Protein A may produce a high background by binding to endogenous immunoglobulin in the tissue, or it may not bind to the species or class of pri-mary antibody employed, we recommend using biotinylated anti-immunoglobulins for routine immunohistochemical staining.

Solid-phase assay of immunoglobulin reactivity normalized to rabbit IgG.
 Product Categories/Family for Protein A, S. aureus, Cowan Strain reagent    BioSeparation; BioSep-Protein A/G/L
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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