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RNASEL recombinant protein :: 2-5A-dependent ribonuclease Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS969669
SDS-PAGE
Unit / Price
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

2-5A-dependent ribonuclease (RNASEL), Recombinant Protein

 Also Known As   

Recombinant Human 2-5A-dependent ribonuclease

 Product Synonym Names    Ribonuclease 4; Ribonuclease L; RNase L
 Product Gene Name   

RNASEL recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    330-741aa; Partial
 Sequence    HPPAEDWKPQ SSHWGAALKD LHRIYRPMIG KLKFFIDEKY KIADTSEGGI YLGFYEKQEV AVKTFCEGSP RAQREVSCLQ SSRENSHLVT FYGSESHRGH LFVCVTLCEQ TLEACLDVHR GEDVENEEDE FARNVLSSIF KAVQELHLSC GYTHQDLQPQ NILIDSKKAA HLADFDKSIK WAGDPQEVKR DLEDLGRLVL YVVKKGSISF EDLKAQSNEE VVQLSPDEET KDLIHRLFHP GEHVRDCLSD LLGHPFFWTW ESRYRTLRNV GNESDIKTRK SESEILRLLQ PGPSEHSKSF DKWTTKINEC VMKKMNKFYE KRGNFYQNTV GDLLKFIRNL GEHIDEEKHK KMKLKIGDPS LYFQKTFPDL VIYVYTKLQN TEYRKHFPQT HSPNKPQCDG AGGASGLASP GC
 OMIM    176807
 3D Structure    ModBase 3D Structure for Q05823
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of RNASEL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for RNASEL recombinant protein

   Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover.
 Product Categories/Family for RNASEL recombinant protein    Epigenetics and Nuclear Signaling
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 SDS-PAGE of RNASEL recombinant protein    RNASEL recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for RNASEL. It may not necessarily be applicable to this product.
 NCBI GI #    10863929
 NCBI GeneID    6041
 NCBI Accession #    NP_066956.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021133.3 [Other Products]
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 UniProt Primary Accession #    Q05823 [Other Products]
 UniProt Secondary Accession #    Q5W0L2; Q6AI46 [Other Products]
 UniProt Related Accession #    Q05823 [Other Products]
 Molecular Weight    63.4kD
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 NCBI Official Full Name    2-5A-dependent ribonuclease
 NCBI Official Synonym Full Names    ribonuclease L
 NCBI Official Symbol    RNASEL [Similar Products]
 NCBI Official Synonym Symbols   
RNS4; PRCA1
[Similar Products]
 NCBI Protein Information    2-5A-dependent ribonuclease
 UniProt Protein Name    2-5A-dependent ribonuclease
 UniProt Synonym Protein Names   
Ribonuclease 4; Ribonuclease L; RNase L
 Protein Family    2-5A-dependent ribonuclease
 UniProt Gene Name    RNASEL [Similar Products]
 UniProt Synonym Gene Names    RNS4; 2-5A-dependent RNase; RNase L [Similar Products]
 UniProt Entry Name    RN5A_HUMAN
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 NCBI Summary for RNASEL    This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RNASEL    RNASEL: a multifunctional enzyme that possesses protein kinase and endoribonuclease activity, Mediator of interferon action, which play a role in mediating resistance to virus infection and apoptosis. Might play a central role in the regulation of mRNA turnover.

Protein type: Ribonuclease; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 3.1.26.-; Other group; Other-Unique family

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytosol; mitochondrial matrix; nuclear matrix

Molecular Function: ATP binding; endoribonuclease activity; metal ion binding; protein binding; protein kinase activity; ribonucleoprotein binding; RNA binding; rRNA binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; fat cell differentiation; mRNA processing; negative regulation of viral genome replication; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of mRNA stability; rRNA processing; transmembrane transport

Disease: Prostate Cancer, Hereditary, 1
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Product References and Citations for RNASEL recombinant protein

   Expression cloning of 2-5A-dependent RNAase a uniquely regulated mediator of interferon action.Zhou A., Hassel B.A., Silverman R.H.Cell 72:753-765(1993) Analysis and origins of the human and mouse RNase L genes mediators of interferon action.Zhou A., Nie H., Silverman R.H.Mamm. Genome 11:989-992(2000) The full-ORF clone resource of the German cDNA consortium.Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.BMC Genomics 8:399-399(2007) The DNA sequence and biological annotation of human chromosome 1.Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.Nature 441:315-321(2006) Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Intrinsic molecular activities of the interferon-induced 2-5A-dependent RNase.Dong B., Xu L., Zhou A., Hassel B.A., Lee X., Torrence P.F., Silverman R.H.J. Biol. Chem. 269:14153-14158(1994) Cloning and characterization of a RNase L inhibitor. A new component of the interferon-regulated 2-5A pathway.Bisbal C., Martinand C., Silhol M., Lebleu B., Salehzada T.J. Biol. Chem. 270:13308-13317(1995) The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells.Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.J. Biol. Chem. 276:48473-48482(2001) ErratumLe Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T.J. Biol. Chem. 277:13354-13354(2002) The 2-5A system in viral infection and apoptosis.Castelli J., Wood K.A., Youle R.J.Biomed. Pharmacother. 52:386-390(1998) Alternative function of a protein kinase homology domain in 2', 5'-oligoadenylate dependent RNase L.Dong B., Silverman R.H.Nucleic Acids Res. 27:439-445(1999) Basis for regulated RNA cleavage by functional analysis of RNase L and Ire1p.Dong B., Niwa M., Walter P., Silverman R.H.RNA 7:361-373(2001) Lysine acetylation targets protein complexes and co-regulates major cellular functions.Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2009) Structural basis for recognition of 2',5'-linked oligoadenylates by human ribonuclease L.Tanaka N., Nakanishi M., Kusakabe Y., Goto Y., Kitade Y., Nakamura K.T.EMBO J. 23:3929-3938(2004) Germline alterations of the RNASEL gene, a candidate HPC1 gene at 1q25, in patients and families with prostate cancer.Roekman A., Ikonen T., Seppaelae E.H., Nupponen N., Autio V., Mononen N., Bailey-Wilson J., Trent J., Carpten J., Matikainen M.P., Koivisto P.A., Tammela T.L.J., Kallioniemi O.-P., Schleutker J.Am. J. Hum. Genet. 70:1299-1304(2002) Germline mutations in the ribonuclease L gene in families showing linkage with HPC1.Carpten J., Nupponen N., Isaacs S., Sood R., Robbins C., Xu J., Faruque M., Moses T., Ewing C., Gillanders E., Hu P., Bujnovszky P., Makalowska I., Baffoe-Bonnie A., Faith D., Smith J., Stephan D., Wiley K., Brownstein M., Gildea D., Kelly B., Jenkins R., Hostetter G., Matikainen M., Schleutker J., Klinger K., Connors T., Xiang Y., Wang Z., De Marzo A., Papadopoulos N., Kallioniemi O.-P., Burk R., Meyers D., Groenberg H., Meltzer P., Silverman R., Bailey-Wilson J., Walsh P., Isaacs W., Trent J.Nat. Genet. 30:181-184(2002) RNASEL Arg462Gln variant is implicated in up to 13% of prostate cancer cases.Casey G., Neville P.J., Plummer S.J., Xiang Y., Krumroy L.M., Klein E.A., Catalona W.J., Nupponen N., Carpten J.D., Trent J.M., Silverman R.H., Witte J.S.Nat. Genet. 32:581-583(2002) Patterns of somatic mutation in human cancer genomes.Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G., Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.Nature 446:153-158(2007)
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 Research Articles on RNASEL    1. Virus infection and RNase L activation disrupt its association with Filamin A and release RNase L to mediate its canonical nuclease-dependent antiviral activities.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RNASEL recombinant proteinPathways associated with RNASEL recombinant protein
 Reference Product  PubMed Publications
 OAS1 recombinant protein  >5 publications with RNASEL and OAS1
 MX1 recombinant protein  >3 publications with RNASEL and MX1
 ISG15 recombinant protein  >2 publications with RNASEL and ISG15
 OASL recombinant protein  >2 publications with RNASEL and OASL
 OAS3 recombinant protein  >1 publications with RNASEL and OAS3
 IRF1 recombinant protein  >1 publications with RNASEL and IRF1
 Products by Pathway  Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
Diseases associated with RNASEL recombinant proteinOrgans/Tissues associated with RNASEL recombinant protein
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >77 publications with RNASEL and Prostatic Neoplasms
 Inflammation Antibodies  >18 publications with RNASEL and Inflammation
 Nervous System Diseases Antibodies  >7 publications with RNASEL and Nervous System Diseases
 Necrosis Antibodies  >4 publications with RNASEL and Necrosis
 Hyperplasia Antibodies  >3 publications with RNASEL and Hyperplasia
 Disease Models, Animal Antibodies  >2 publications with RNASEL and Disease Models, Animal
 Liver Diseases Antibodies  >2 publications with RNASEL and Liver Diseases
 Ovarian Diseases Antibodies  >1 publications with RNASEL and Ovarian Diseases
 Fibrosis Antibodies  >1 publications with RNASEL and Fibrosis
 PROSTATE CANCER, HEREDITARY, 1 Antibodies  >1 publications with RNASEL and PROSTATE CANCER, HEREDITARY, 1
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >94 publications with RNASEL and Prostate
 Kidney Antibodies  >4 publications with RNASEL and Kidney
 Lung Antibodies  >3 publications with RNASEL and Lung
 Vascular Antibodies  >3 publications with RNASEL and Vascular
 Skin Antibodies  >2 publications with RNASEL and Skin
 Brain Antibodies  >2 publications with RNASEL and Brain
 Bone Antibodies  >2 publications with RNASEL and Bone
 Uncharacterized Tissue Antibodies  >1 publications with RNASEL and Uncharacterized Tissue
 Mouth Antibodies  >1 publications with RNASEL and Mouth
 Bladder Antibodies  >1 publications with RNASEL and Bladder
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