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PRKAA1 recombinant protein :: 5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1476756 PRKAA1 recombinant protein
Unit / Price
0.05 mg (E-Coli)  /  $985 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $1,045 +1 FREE 8GB USB
0.05 mg (Baculovirus)  /  $1,175 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $1,320 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $1,420 +1 FREE 8GB USB
0.05 mg (Mammalian-Cell)  /  $1,425 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $1,455 +1 FREE 8GB USB
0.1 mg (Baculovirus)  /  $1,490 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $1,605 +2 FREE 8GB USB
1 mg (E-Coli)  /  $2,070 +2 FREE 8GB USB
0.5 mg (Baculovirus)  /  $2,130 +2 FREE 8GB USB
0.1 mg (Mammalian-Cell)  /  $2,145 +2 FREE 8GB USB
1 mg (Yeast)  /  $2,445 +3 FREE 8GB USB
1 mg (Baculovirus)  /  $2,800 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1), Recombinant Protein

 Also Known As   

Recombinant Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1)

 Product Synonym Names    5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; EC=2.7.11.1; Tau-protein kinase PRKAA1; EC=2.7.11.26
 Product Gene Name   

PRKAA1 recombinant protein

[Similar Products]
 Product Synonym Gene Name    PRKAA1; AMPK1 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    1-559aa; full length protein
 Sequence    MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC ANLIKILAQ
 OMIM    602739
 3D Structure    ModBase 3D Structure for Q13131
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    >90%
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Homo sapiens (Human)
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 Preparation and Storage    Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of PRKAA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PRKAA1 recombinant protein

   This protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5 -prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
NCBI/Uniprot data below describe general gene information for PRKAA1. It may not necessarily be applicable to this product.
 NCBI GI #    94557301
 NCBI GeneID    5562
 NCBI Accession #    NP_006242.5 [Other Products]
 NCBI GenBank Nucleotide #    NM_006251.5 [Other Products]
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 UniProt Primary Accession #    Q13131 [Other Products]
 UniProt Secondary Accession #    O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1 [Other Products]
 UniProt Related Accession #    Q13131 [Other Products]
 Molecular Weight    65,523 Da
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 NCBI Official Full Name    5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
 NCBI Official Synonym Full Names    protein kinase, AMP-activated, alpha 1 catalytic subunit
 NCBI Official Symbol    PRKAA1 [Similar Products]
 NCBI Official Synonym Symbols   
AMPK; AMPKa1
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase kinase; AMP-activated protein kinase, catalytic, alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain
 UniProt Protein Name    5'-AMP-activated protein kinase catalytic subunit alpha-1
 UniProt Synonym Protein Names   
Acetyl-CoA carboxylase kinase (EC:2.7.11.27); ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31); HMGCR kinase; Tau-protein kinase PRKAA1 (EC:2.7.11.26)
 UniProt Gene Name    PRKAA1 [Similar Products]
 UniProt Synonym Gene Names    AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase [Similar Products]
 UniProt Entry Name    AAPK1_HUMAN
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 NCBI Summary for PRKAA1    The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRKAA1    AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11.1; EC 2.7.11.27; Autophagy; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: nucleoplasm; cytoplasm; apical plasma membrane; intracellular; cytosol; nucleus; AMP-activated protein kinase complex

Molecular Function: protein C-terminus binding; AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; metal ion binding; tau-protein kinase activity; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; chromatin binding; kinase binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; protein kinase activity

Biological Process: activation of MAPK activity; protein heterooligomerization; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; signal transduction; glucose homeostasis; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of cell proliferation; response to gamma radiation; negative regulation of glucosylceramide biosynthetic process; cell cycle arrest; positive regulation of autophagy; fatty acid biosynthetic process; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; cold acclimation; positive regulation of glycolysis; insulin receptor signaling pathway; response to hypoxia; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis
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 Research Articles on PRKAA1    1. AMPK activators ameliorated carrageenan-induced insulin resistance via SeP inhibition.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRKAA1 recombinant proteinPathways associated with PRKAA1 recombinant protein
 Reference Product  PubMed Publications
 PRKAG1 recombinant protein  >18 publications with PRKAA1 and PRKAG1
 PRKAB1 recombinant protein  >17 publications with PRKAA1 and PRKAB1
 PRKAB2 recombinant protein  >16 publications with PRKAA1 and PRKAB2
 MTOR recombinant protein  >15 publications with PRKAA1 and MTOR
 PRKAG3 recombinant protein  >13 publications with PRKAA1 and PRKAG3
 CFTR recombinant protein  >2 publications with PRKAA1 and CFTR
 ACACA recombinant protein  >2 publications with PRKAA1 and ACACA
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Diseases associated with PRKAA1 recombinant proteinOrgans/Tissues associated with PRKAA1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >26 publications with PRKAA1 and Neoplasms
 Cardiovascular Diseases Antibodies  >11 publications with PRKAA1 and Cardiovascular Diseases
 Death Antibodies  >10 publications with PRKAA1 and Death
 Heart Diseases Antibodies  >9 publications with PRKAA1 and Heart Diseases
 Fatty Liver Antibodies  >8 publications with PRKAA1 and Fatty Liver
 Necrosis Antibodies  >7 publications with PRKAA1 and Necrosis
 Inflammation Antibodies  >7 publications with PRKAA1 and Inflammation
 Insulin Resistance Antibodies  >6 publications with PRKAA1 and Insulin Resistance
 Nervous System Diseases Antibodies  >4 publications with PRKAA1 and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with PRKAA1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >42 publications with PRKAA1 and Muscle
 Liver Antibodies  >19 publications with PRKAA1 and Liver
 Blood Antibodies  >18 publications with PRKAA1 and Blood
 Vascular Antibodies  >13 publications with PRKAA1 and Vascular
 Adipose Tissue Antibodies  >12 publications with PRKAA1 and Adipose Tissue
 Brain Antibodies  >10 publications with PRKAA1 and Brain
 Lung Antibodies  >9 publications with PRKAA1 and Lung
 Pancreas Antibodies  >7 publications with PRKAA1 and Pancreas
 Placenta Antibodies  >5 publications with PRKAA1 and Placenta
 Connective Tissue Antibodies  >5 publications with PRKAA1 and Connective Tissue
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