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ADAM10 recombinant protein :: A Disintegrin And Metalloprotease 10 (ADAM10) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009917 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $190 +1 FREE 8GB USB
0.05 mg  /  $400 +1 FREE 8GB USB
0.1 mg  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 10 (ADAM10), Recombinant Protein

 Also Known As   

Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

 Product Gene Name   

ADAM10 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-R ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD V
 Chromosome Location    Chromosome: 15; NC_000015.9 (58887403..59042177, complement). Location: 15q22
 OMIM    602192
 3D Structure    ModBase 3D Structure for O14672
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Arg420~Val641 (Accession # O14672) with N-terminal His-Tag
 Subcellular Location    Cell membrane; Single-pass type I membrane protein. Endomembrane system
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    6.5
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ADAM10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for ADAM10 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ADAM10 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ADAM10 recombinant protein    ADAM10 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
 NCBI GI #    4557251
 NCBI GeneID    102
 NCBI Accession #    NP_001101.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001110.3 [Other Products]
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 UniProt Primary Accession #    O14672 [Other Products]
 UniProt Secondary Accession #    Q10742; Q92650 [Other Products]
 UniProt Related Accession #    O14672 [Other Products]
 Molecular Weight    25.7kDa
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 NCBI Official Full Name    disintegrin and metalloproteinase domain-containing protein 10
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 10
 NCBI Official Symbol    ADAM10 [Similar Products]
 NCBI Official Synonym Symbols   
RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 10
 UniProt Synonym Protein Names   
CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM10 [Similar Products]
 UniProt Synonym Gene Names    KUZ; MADM; ADAM 10 [Similar Products]
 UniProt Entry Name    ADA10_HUMAN
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 NCBI Summary for ADAM10    Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADAM10    ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Protease; Cell surface; Vesicle; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.81

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; focal adhesion; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; integral to membrane; plasma membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; SH3 domain binding; protein kinase binding; receptor binding

Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; cell-cell signaling; monocyte activation; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
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 Research Articles on ADAM10    1. the proteolytic activity of ADAM10/17 is essential for regulating HGF/c-Met signalling during acute liver damage and following regeneration.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAM10 recombinant proteinPathways associated with ADAM10 recombinant protein
 Reference Product  PubMed Publications
 EGFR recombinant protein  >30 publications with ADAM10 and EGFR
 CTNNB1 recombinant protein  >14 publications with ADAM10 and CTNNB1
 NOTCH1 recombinant protein  >14 publications with ADAM10 and NOTCH1
 CDH2 recombinant protein  >5 publications with ADAM10 and CDH2
 NOTCH3 recombinant protein  >2 publications with ADAM10 and NOTCH3
 NOTCH2 recombinant protein  >2 publications with ADAM10 and NOTCH2
 DLL1 recombinant protein  >2 publications with ADAM10 and DLL1
 JAG1 recombinant protein  >1 publications with ADAM10 and JAG1
 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway antibodies  Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
Diseases associated with ADAM10 recombinant proteinOrgans/Tissues associated with ADAM10 recombinant protein
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >187 publications with ADAM10 and Necrosis
 Neoplasms Antibodies  >125 publications with ADAM10 and Neoplasms
 Brain Diseases Antibodies  >112 publications with ADAM10 and Brain Diseases
 Inflammation Antibodies  >53 publications with ADAM10 and Inflammation
 Skin Diseases Antibodies  >28 publications with ADAM10 and Skin Diseases
 Cardiovascular Diseases Antibodies  >19 publications with ADAM10 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >17 publications with ADAM10 and Vascular Diseases
 Gastrointestinal Diseases Antibodies  >17 publications with ADAM10 and Gastrointestinal Diseases
 Kidney Diseases Antibodies  >17 publications with ADAM10 and Kidney Diseases
 Breast Neoplasms Antibodies  >17 publications with ADAM10 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >136 publications with ADAM10 and Brain
 Blood Antibodies  >101 publications with ADAM10 and Blood
 Vascular Antibodies  >45 publications with ADAM10 and Vascular
 Kidney Antibodies  >36 publications with ADAM10 and Kidney
 Embryonic Tissue Antibodies  >33 publications with ADAM10 and Embryonic Tissue
 Muscle Antibodies  >27 publications with ADAM10 and Muscle
 Nerve Antibodies  >23 publications with ADAM10 and Nerve
 Bone Antibodies  >21 publications with ADAM10 and Bone
 Skin Antibodies  >19 publications with ADAM10 and Skin
 Lung Antibodies  >18 publications with ADAM10 and Lung
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