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ADAM28 recombinant protein :: A Disintegrin And Metalloprotease 28 (ADAM28) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010562 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $265 +1 FREE 8GB USB
0.1 mg  /  $410 +1 FREE 8GB USB
0.2 mg  /  $505 +1 FREE 8GB USB
0.5 mg  /  $980 +1 FREE 8GB USB
1 mg  /  $1,445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 28 (ADAM28), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)

 Product Gene Name   

ADAM28 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-EVADKS CYNRNEGGSK YGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK
 Chromosome Location    Chromosome: 8; NC_000008.10 (24151553..24212726). Location: 8p21.2
 OMIM    606188
 3D Structure    ModBase 3D Structure for Q9UKQ2
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 Host    E Coli
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 Purity/Purification    >98%
 Form/Format    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag
 Tissue Specificity    Spleen, Stomach, Intestine, Brain.
 Subcellular Location    Cell membrane; Single-pass type I membrane protein.
 Traits    Freeze-dried powder
 Predicted isoelectric point    7.5
 Predicted Molecular Mass    29.3kDa
 Accurate Molecular Mass    34kDa as determined by SDS-PAGE reducing conditions.
 Phenomenon explanation    The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ADAM28 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for ADAM28 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

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 SDS-PAGE of ADAM28 recombinant protein    ADAM28 recombinant protein SDS-PAGE image
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 Test Data of ADAM28 recombinant protein    ADAM28 recombinant protein Test Data image
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NCBI/Uniprot data below describe general gene information for ADAM28. It may not necessarily be applicable to this product.
 NCBI GI #    98985828
 NCBI GeneID    10863
 NCBI Accession #    NP_055080.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014265.4 [Other Products]
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 UniProt Primary Accession #    Q9UKQ2 [Other Products]
 UniProt Secondary Accession #    Q9Y339; Q9Y3S0; B2RMV5 [Other Products]
 UniProt Related Accession #    Q9UKQ2 [Other Products]
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 NCBI Official Full Name    disintegrin and metalloproteinase domain-containing protein 28 isoform 1 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 28
 NCBI Official Symbol    ADAM28 [Similar Products]
 NCBI Official Synonym Symbols   
MDCL; MDC-L; ADAM23; MDC-Lm; MDC-Ls; eMDCII; ADAM 28; eMDC II
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 28; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 28
 UniProt Synonym Protein Names   
Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; eMDC II; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM28 [Similar Products]
 UniProt Synonym Gene Names    ADAM23; MDCL; ADAM 28; eMDC II; MDC-L [Similar Products]
 UniProt Entry Name    ADA28_HUMAN
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 NCBI Summary for ADAM28    This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
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 UniProt Comments for ADAM28    ADAM28: May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: integral to membrane; extracellular region; plasma membrane

Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: spermatogenesis; proteolysis
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 Research Articles on ADAM28    1. The change of ADAM28 and IGFBP-3 gene expression is present in the normal tissue in overweight/obese patients with colorectal cancer only, and the observed variability of ADAM28 and IGFBP-3 expression may be an initial process of cancer proliferation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAM28 recombinant proteinDiseases associated with ADAM28 recombinant protein
 Reference Product  PubMed Publications
 ADAMDEC1 recombinant protein  >4 publications with ADAM28 and ADAMDEC1
 LGI1 recombinant protein  >1 publications with ADAM28 and LGI1
 ITGB1 recombinant protein  >1 publications with ADAM28 and ITGB1
 ITGA4 recombinant protein  >1 publications with ADAM28 and ITGA4
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >6 publications with ADAM28 and Breast Neoplasms
 Adenocarcinoma Antibodies  >5 publications with ADAM28 and Adenocarcinoma
 Inflammation Antibodies  >4 publications with ADAM28 and Inflammation
 Neoplasms, Experimental Antibodies  >4 publications with ADAM28 and Neoplasms, Experimental
 Lymphoma Antibodies  >3 publications with ADAM28 and Lymphoma
 Liver Diseases Antibodies  >2 publications with ADAM28 and Liver Diseases
 Disease Models, Animal Antibodies  >1 publications with ADAM28 and Disease Models, Animal
 Kidney Diseases Antibodies  >1 publications with ADAM28 and Kidney Diseases
 Congenital Abnormalities Antibodies  >1 publications with ADAM28 and Congenital Abnormalities
 Atherosclerosis Antibodies  >1 publications with ADAM28 and Atherosclerosis
Organs/Tissues associated with ADAM28 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with ADAM28 and Blood
 Lung Antibodies  >14 publications with ADAM28 and Lung
 Lymph Node Antibodies  >6 publications with ADAM28 and Lymph Node
 Lymph Antibodies  >6 publications with ADAM28 and Lymph
 Mouth Antibodies  >6 publications with ADAM28 and Mouth
 Kidney Antibodies  >3 publications with ADAM28 and Kidney
 Connective Tissue Antibodies  >2 publications with ADAM28 and Connective Tissue
 Bladder Antibodies  >2 publications with ADAM28 and Bladder
 Embryonic Tissue Antibodies  >2 publications with ADAM28 and Embryonic Tissue
 Testis Antibodies  >1 publications with ADAM28 and Testis
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