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ADAMTS12 recombinant protein :: A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010993 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $145 +1 FREE 8GB USB
0.05 mg  /  $280 +1 FREE 8GB USB
0.1 mg  /  $435 +1 FREE 8GB USB
0.2 mg  /  $535 +1 FREE 8GB USB
0.5 mg  /  $1,035 +1 FREE 8GB USB
1 mg  /  $1,540 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

 Product Gene Name   

ADAMTS12 recombinant protein

[Similar Products]
 Matching Pairs    Antibody: A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12) (MBS2006303)
Antigen: A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12) (MBS2010993)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-KLLY FWQFGRWTEC SVTCGTGIRR QAAHCVKKGH GIVKTTFCNP ETQPSVRQKK CHEKDCPPRW WAGEWEACST TCGPYGEKKR TVLCIQTMGS DEQALPATDC QHLLKPKALV SCNRDILCPS DWTVGNWSEC SVSCGGGVRI RSVTCAKNLN EPCDKTRKPN SRALCGLQQC P
 Chromosome Location    Chromosome: 2; NC_000002.11 (179390716..179672150, complement). Location: 2q31
 OMIM    188840
 3D Structure    ModBase 3D Structure for Q8WZ42
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Lys827~Pro1001 (Accession # Q8WZ42) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of ADAMTS12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ADAMTS12 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ADAMTS12 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ADAMTS12 recombinant protein    ADAMTS12 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ADAMTS12. It may not necessarily be applicable to this product.
 NCBI GI #    378925625
 NCBI GeneID    7273
 NCBI Accession #    NP_001243779.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256850.1 [Other Products]
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 UniProt Primary Accession #    Q8WZ42 [Other Products]
 UniProt Secondary Accession #    Q10465; Q10466; Q15598; Q2XUS3; Q32Q60; Q4U1Z6; Q4ZG20; A6NKB1; E7EQE6; E7ET18; K7ENY1 [Other Products]
 UniProt Related Accession #    Q8WZ42 [Other Products]
 Molecular Weight    21.0kDa
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 NCBI Official Full Name    titin isoform N2BA
 NCBI Official Synonym Full Names    titin
 NCBI Official Symbol    TTN [Similar Products]
 NCBI Official Synonym Symbols   
TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; LGMD2J
[Similar Products]
 NCBI Protein Information    titin; connectin; rhabdomyosarcoma antigen MU-RMS-40.14
 UniProt Protein Name    Titin
 UniProt Synonym Protein Names   
Connectin; Rhabdomyosarcoma antigen MU-RMS-40.14
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    TTN [Similar Products]
 UniProt Entry Name    TITIN_HUMAN
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 NCBI Summary for ADAMTS12    This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]
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 UniProt Comments for ADAMTS12    Function: Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase. Ref.28

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Full activation of the protein kinase domain requires both phosphorylation of Tyr-32341, preventing it from blocking the catalytic aspartate residue, and binding of Ca/CALM to the C-terminal regulatory tail of the molecule which results in ATP binding to the kinase. Ref.28

Subunit structure: Interacts with MYOM1, MYOM2, tropomyosin and myosin. Interacts with actin, primarily via the PEVK domains and with MYPN

By similarity. Interacts with FHL2, NEB, CRYAB, LMNA/lamin-A and LMNB/lamin-B. Interacts with TCAP/telethonin and/or ANK1 isoform Mu17/ank15, via the first two N-terminal immunoglobulin domains. Interacts with TRIM63 and TRIM55, through several domains including immunoglobulin domains 141 and 142. Interacts with ANKRD1, ANKRD2 and ANKRD23, via the region between immunoglobulin domains 77 and 78 and interacts with CAPN3, via immunoglobulin domain 79. Interacts with NBR1 through the protein kinase domain. Interacts with CALM/calmodulin. Isoform 6 interacts with OBSCN isoform 3. Interacts with CMYA5. Ref.3 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.26 Ref.28 Ref.38

Subcellular location: Cytoplasm

Probable. Nucleus Ref.23.

Tissue specificity: Isoforms 3, 7 and 8 are expressed in cardiac muscle. Isoform 4 is expressed in vertebrate skeletal muscle. Isoform 6 is expressed in skeletal muscle (at protein level). Ref.3 Ref.7

Domain: ZIS1 and ZIS5 regions contain multiple SPXR consensus sites for ERK- and CDK-like protein kinases as well as multiple SP motifs. ZIS1 could adopt a closed conformation which would block the TCAP-binding site.The PEVK region may serve as an entropic spring of a chain of structural folds and may also be an interaction site to other myofilament proteins to form interfilament connectivity in the sarcomere.

Post-translational modification: Autophosphorylated

By similarity. Ref.14 Ref.28

Involvement in disease: Hereditary myopathy with early respiratory failure (HMERF) [MIM:603689]: Autosomal dominant, adult-onset myopathy with early respiratory muscle involvement.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38Cardiomyopathy, familial hypertrophic 9 (CMH9) [MIM:613765]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32Cardiomyopathy, dilated 1G (CMD1G) [MIM:604145]: A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.34 Ref.35 Ref.37Tardive tibial muscular dystrophy (TMD) [MIM:600334]: Autosomal dominant, late-onset distal myopathy. Muscle weakness and atrophy are usually confined to the anterior compartment of the lower leg, in particular the tibialis anterior muscle. Clinical symptoms usually occur at age 35-45 years or much later.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.33 Ref.36Limb-girdle muscular dystrophy 2J (LGMD2J) [MIM:608807]: An autosomal recessive degenerative myopathy characterized by progressive weakness of the pelvic and shoulder girdle muscles. Severe disability is observed within 20 years of onset.Note: The disease is caused by mutations affecting the gene represented in this entry.Early-onset myopathy with fatal cardiomyopathy (EOMFC) [MIM:611705]: Early-onset myopathies are inherited muscle disorders that manifest typically from birth or infancy with hypotonia, muscle weakness, and delayed motor development. EOMFC is a titinopathy that, in contrast with the previously described examples, involves both heart and skeletal muscle, has a congenital onset, and is purely recessive. This phenotype is due to homozygous out-of-frame TTN deletions, which lead to a total absence of titin's C-terminal end from striated muscles and to secondary CAPN3 depletion.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.39

Miscellaneous: In some isoforms, after the PEVK repeat region there is a long PEVK duplicated region. On account of this region, it has been very difficult to sequence the whole protein. The length of this region (ranging from 183 to 2174 residues), may be a key elastic element of titin.

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Contains 132 fibronectin type-III domains.Contains 152 Ig-like (immunoglobulin-like) domains.Contains 19 Kelch repeats.Contains 1 protein kinase domain.Contains 17 RCC1 repeats.Contains 14 TPR repeats.Contains 15 WD repeats.

Sequence caution: The sequence AAH58824.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 553.The sequence AAH70170.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 627.The sequence CAA62188.1 differs from that shown. Reason: Frameshift at positions 17036 and 17043. The sequence CAD12455.1 differs from that shown. Reason: Frameshift at positions 17036 and 17043.
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 Research Articles on ADAMTS12    1. novel titin Ig-calcium interaction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADAMTS12 recombinant proteinDiseases associated with ADAMTS12 recombinant protein
 Products by Pathway  Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >118 publications with ADAMTS12 and Cardiovascular Diseases
 Neoplasms Antibodies  >77 publications with ADAMTS12 and Neoplasms
 Muscular Diseases Antibodies  >74 publications with ADAMTS12 and Muscular Diseases
 Cardiomyopathies Antibodies  >73 publications with ADAMTS12 and Cardiomyopathies
 Cardiomyopathy, Dilated Antibodies  >58 publications with ADAMTS12 and Cardiomyopathy, Dilated
 Muscle Weakness Antibodies  >25 publications with ADAMTS12 and Muscle Weakness
 Hypertrophy Antibodies  >21 publications with ADAMTS12 and Hypertrophy
 Necrosis Antibodies  >9 publications with ADAMTS12 and Necrosis
 Hereditary Myopathy with Early Respiratory Failure Antibodies  >8 publications with ADAMTS12 and Hereditary Myopathy with Early Respiratory Failure
 Hyperplasia Antibodies  >8 publications with ADAMTS12 and Hyperplasia
Organs/Tissues associated with ADAMTS12 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >593 publications with ADAMTS12 and Muscle
 Heart Antibodies  >205 publications with ADAMTS12 and Heart
 Blood Antibodies  >90 publications with ADAMTS12 and Blood
 Thymus Antibodies  >44 publications with ADAMTS12 and Thymus
 Lung Antibodies  >39 publications with ADAMTS12 and Lung
 Brain Antibodies  >32 publications with ADAMTS12 and Brain
 Embryonic Tissue Antibodies  >31 publications with ADAMTS12 and Embryonic Tissue
 Nerve Antibodies  >15 publications with ADAMTS12 and Nerve
 Thyroid Antibodies  >14 publications with ADAMTS12 and Thyroid
 Skin Antibodies  >13 publications with ADAMTS12 and Skin
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