Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow ADAMTS13  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • TFAP2A Antibody
 • CsgBAC operon transcriptional regulatory protein (csgD) Recombinant Protein
 • Follistatin-related protein 1 (Fstl1) ELISA Kit
 • 14-3-3 TAU-THETA Antibody
 • b-Amyloid Antibody
 • PEMT Antibody
 • Nerve Growth Factor (NGF) ELISA Kit
 • SYP - Synaptophysin Antibody
 • UBE2S Antibody
 • FSH Receptor - FSHR Antibody
 • TLR3 Antibody
 • Macrophage Inflammatory Protein 1 Alpha (MIP1a) Antibody
 • PDGFRb Antibody
 • cystinosis, nephropathic (CTNS) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ADAMTS13 recombinant protein

Scan QR to view Datasheet Catalog #    MBS636953 ADAMTS13 recombinant protein
Unit / Price
0.02 mg  /  $710 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAMTS13, Recombinant Protein

★Popular Item★
 Also Known As   

ADAMTS13, Recombinant, Human (A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13)

 Product Gene Name   

ADAMTS13 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: 9; NC_000009.11 (136279459..136324525). Location: 9q34
 OMIM    274150
 3D Structure    ModBase 3D Structure for Q76LX8
Section Bar
 Host    CHO
Section Bar
 Specificity    Human ADAMTS13.
 Purity/Purification    >90%, as determined by SDS-PAGE and visualized by silver stain.
 Form/Format    Supplied as a liquid in 25mM sodium acetate, 1M sodium chloride, 10mM CaCl2 pH 5.5
 Concentration    0.34mg/ml (lot specific)
Section Bar
 Immunogen    CHO-derived recombinant human ADAMTS13.
 Activity    Measured by its ability to cleave recombinant human von Willebrand factor A2 domain. Under the described conditions, 0.5ug of rhADAMTS13 can cleave > 50% of 5ug of rhvWF-A2.
The rhvWF-A2 cleaving activity can be inhibited by 5 mM 1,10-phenanthroline.
 N-terminal Sequence Analysis    No results obtained; Gln34 predicted.
 Molecular Mass    Predicted: 73kD
Observed: 90kD (reducing conditions)
 Biological Activity    Measured by its ability to cleave the fluorogenic peptide substrate, FRETS-VWF73. The specific activity is >10pmol/min/ug.
 Grade    Purified
 Endotoxin    < 1.0 EU per 1ug
Section Bar
 Preparation and Storage    Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping: Dry Ice.
 Other Notes    Small volumes of ADAMTS13 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for ADAMTS13 recombinant protein

   ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13), also known as von Willebran Factor (vWF) cleaving protease, is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cystein-rich, spacer, a second set of seven TSP1 repeats, and two CUB domins. The only known substrate of ADAMTS13 is vWF, a blood glycoprotein with two homeostatic functions (4). It is required for platelet adhesion to sites of vascular damage and acts as a carrier protein for blood-clotting factor VIII in the circulation. It exists in plasma as multimers, the largest of which effectively mediate platelet adhesion. ADAMTS13 cleaves multimeric vWF in the A2 domain at the position, Tyr 1605-Met 1606. Defect in ADAMTS13 activity is a cause of congenital thrombotic thrombocytopenic purpura (TTP), also known as Upshaw-Schulman syndrome. Lack of ADAMTS13 activity allows unusually large vWF (UlvWF) to occur in plasma (5). These UlvWF multimers have tendency to agglutinate circulating platelets at sites with high levels of shear stress to cause TTP.

Recombinant protein corresponding to Gln34-Trp688 with a C-terminal 10X-His tag from human ADAMTS13 (Accession #NP_620594), expressed in CHO cell line. The purified rhADAMTS13 starts at the N-terminus of the pro domain and ends in the spacer domain.
 Product Categories/Family for ADAMTS13 recombinant protein    Molecular Biology; MB-Matrix Metalloproteinases
 Applications Tested/Suitable for ADAMTS13 recombinant protein   

Western Blot (WB)

 Application Notes for ADAMTS13 recombinant protein    Suitable for use in Western Blot.
Dilution: Western Blot: 20ug/ml
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ADAMTS13. It may not necessarily be applicable to this product.
 NCBI GI #    73695936
 NCBI GeneID    11093
 NCBI Accession #    NP_620596.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_139027.4 [Other Products]
Section Bar
 UniProt Primary Accession #    Q76LX8 [Other Products]
 UniProt Secondary Accession #    Q6UY16; Q710F6; Q711T8; Q96L37; Q9H0G3 [Other Products]
 UniProt Related Accession #    Q76LX8 [Other Products]
 Molecular Weight    ~73kD [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 13
 NCBI Official Symbol    ADAMTS13 [Similar Products]
 NCBI Official Synonym Symbols   
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 13
 UniProt Synonym Protein Names   
von Willebrand factor-cleaving protease
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    ADAMTS13 [Similar Products]
 UniProt Synonym Gene Names    C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP [Similar Products]
 UniProt Entry Name    ATS13_HUMAN
Section Bar
 NCBI Summary for ADAMTS13    This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Section Bar
 UniProt Comments for ADAMTS13    ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.24.87; Extracellular matrix; Secreted, signal peptide; Calcium-binding; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: integrin-mediated signaling pathway; platelet activation; protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein metabolic process; response to toxin; cell-matrix adhesion; peptide catabolic process; protein processing; proteolysis; post-translational protein modification

Disease: Thrombotic Thrombocytopenic Purpura, Congenital
Table BarTOPTable Bar
 Research Articles on ADAMTS13    1. Novel frameshift mutations in ADAMTS13 in two families with hereditary thrombotic thrombocytopenic purpura.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ADAMTS13 recombinant proteinDiseases associated with ADAMTS13 recombinant protein
 Reference Product  PubMed Publications
 VWF recombinant protein  >551 publications with ADAMTS13 and VWF
 ABO recombinant protein  >26 publications with ADAMTS13 and ABO
 CFHR1 recombinant protein  >2 publications with ADAMTS13 and CFHR1
 CFHR3 recombinant protein  >1 publications with ADAMTS13 and CFHR3
 GP1BA recombinant protein  >1 publications with ADAMTS13 and GP1BA
 Disease Name  Pubmed Publications
 Purpura, Thrombotic Thrombocytopenic Antibodies  >864 publications with ADAMTS13 and Purpura, Thrombotic Thrombocytopenic
 Thrombotic Microangiopathies Antibodies  >789 publications with ADAMTS13 and Thrombotic Microangiopathies
 Kidney Diseases Antibodies  >205 publications with ADAMTS13 and Kidney Diseases
 Purpura, Thrombocytopenic, Idiopathic Antibodies  >151 publications with ADAMTS13 and Purpura, Thrombocytopenic, Idiopathic
 Inflammation Antibodies  >62 publications with ADAMTS13 and Inflammation
 Fever Antibodies  >61 publications with ADAMTS13 and Fever
 Liver Diseases Antibodies  >41 publications with ADAMTS13 and Liver Diseases
 Necrosis Antibodies  >21 publications with ADAMTS13 and Necrosis
 Hepatitis Antibodies  >20 publications with ADAMTS13 and Hepatitis
 Liver Failure Antibodies  >18 publications with ADAMTS13 and Liver Failure
Organs/Tissues associated with ADAMTS13 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >853 publications with ADAMTS13 and Blood
 Kidney Antibodies  >103 publications with ADAMTS13 and Kidney
 Liver Antibodies  >87 publications with ADAMTS13 and Liver
 Brain Antibodies  >49 publications with ADAMTS13 and Brain
 Connective Tissue Antibodies  >15 publications with ADAMTS13 and Connective Tissue
 Nerve Antibodies  >5 publications with ADAMTS13 and Nerve
 Ovary Antibodies  >4 publications with ADAMTS13 and Ovary
 Intestine Antibodies  >3 publications with ADAMTS13 and Intestine
 Placenta Antibodies  >1 publications with ADAMTS13 and Placenta
 Eye Antibodies  >1 publications with ADAMTS13 and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions