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pcrA recombinant protein :: ATP-dependent DNA helicase PcrA Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1006903
SDS-PAGE
Unit / Price
0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
0.01 mg (Yeast)  /  $125 +1 FREE 8GB USB
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.05 mg (Yeast)  /  $220 +1 FREE 8GB USB
0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
0.1 mg (Yeast)  /  $350 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.2 mg (Yeast)  /  $560 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
0.5 mg (Yeast)  /  $920 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
1 mg (Yeast)  /  $1,445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ATP-dependent DNA helicase PcrA, Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Geobacillus stearothermophilus ATP-dependent DNA helicase PcrA

 Product Synonym Names    Geobacillus stearothermophilus (Bacillus stearothermophilus)
 Product Gene Name   

pcrA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1006903 COA    COA PDF
 Sequence Positions    10-289aa; Partial
 Sequence    AHLNKEQQEA VRTTEGPLLI MAGAGSGKTR VLTHRIAYLM AEKHVAPWNI LAITFTNKAA REMRERVQSL LGGAAEDVWI STFHSMCVRI LRRDIDRIGI NRNFSILDPT DQLSVMKTIL KEKNIDPKKF EPRTILGTIS AAKNELLPPE QFAKRASTYY EKVVSDVYQE YQQRLLRNHS LDFDDLIMTT IQLFDRVPDV LHYYQYKFQY IHIDEYQDTN RAQYTLVKKL AERFQNICAV GDADQSIYRW RGADIQNILS FERDYPNAKV ILLEQNYRST
 3D Structure    ModBase 3D Structure for P56255
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 Host    E Coli or Yeast or Baculovirus or Mammalian Cell
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Species    Geobacillus stearothermophilus (Bacillus stearothermophilus)
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of pcrA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for pcrA recombinant protein

   DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
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 SDS-PAGE of pcrA recombinant protein    pcrA recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for pcrA. It may not necessarily be applicable to this product.
 NCBI GI #    3024353
 NCBI Accession #    P56255.1 [Other Products]
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 UniProt Primary Accession #    P56255 [Other Products]
 Molecular Weight    48.57kD
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 NCBI Official Full Name    ATP-dependent DNA helicase PcrA
 UniProt Protein Name    ATP-dependent DNA helicase PcrA
 Protein Family    ATP-dependent DNA helicase
 UniProt Gene Name    pcrA [Similar Products]
 UniProt Entry Name    PCRA_GEOSE
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 UniProt Comments for pcrA    DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
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Product References and Citations for pcrA recombinant protein

   "Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism." Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B. Nucleic Acids Res. 26:2686-2693(1998)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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