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AURORA B recombinant protein

Scan QR to view Datasheet Catalog #    MBS515183
SDS-PAGE
Unit / Price
0.02 mg  /  $260 +1 FREE 8GB USB
0.05 mg  /  $415 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AURORA B, Recombinant Protein

 Also Known As   

AURORA B, Unactive recombinant protein

 Product Synonym Names    AURKB; AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12
 Product Gene Name   

AURORA B recombinant protein

[Similar Products]
 Product Synonym Gene Name    AURKB; AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 17; NC_000017.10 (8108049..8113944, complement). Location: 17p13.1
 OMIM    604970
 3D Structure    ModBase 3D Structure for Q96GD4
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 Host    Sf9 insect cells
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 Form/Format    50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
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 Type    Recombinant Fusion Protein
 Species    Human
 Tag Information    GST tag
 Expression System    Sf9 insect cells using baculovirus
 Source Note    Recombinant full-length human AURORA B was expressed by baculovirus in Sf9 insect cells
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 Preparation and Storage    Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
 Other Notes    Small volumes of AURORA B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for AURORA B recombinant protein

   Recombinant full-length human AURORA B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
 Product Categories/Family for AURORA B recombinant protein    Unactive Kinase; Signaling Proteins - Unactive Kinases
 Applications Tested/Suitable for AURORA B recombinant protein   

Kinase Assay, Western Blot (WB)

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 SDS-PAGE of AURORA B recombinant protein    AURORA B recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for AURORA B. It may not necessarily be applicable to this product.
 NCBI GI #    378786659
 NCBI GeneID    9212
 NCBI Accession #    NP_004208.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004217 [Other Products]
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 UniProt Primary Accession #    Q96GD4 [Other Products]
 UniProt Secondary Accession #    O14630; O60446; O95083; Q96DV5; Q9UQ46; B4DNM4; C7G533; C7G534; C7G535; D3DTR4 [Other Products]
 UniProt Related Accession #    Q96GD4 [Other Products]
 Molecular Weight    ~68 kDa
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 NCBI Official Full Name    Homo sapiens aurora kinase B (AURKB), transcript variant 1, mRNA
 NCBI Official Synonym Full Names    aurora kinase B
 NCBI Official Symbol    AURKB [Similar Products]
 NCBI Official Synonym Symbols   
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
[Similar Products]
 NCBI Protein Information    aurora kinase B; ARK-2; STK-1; aurora 1; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora-related kinase 2; serine/threonine kinase 12; aurora/IPL1-related kinase 2; serine/threonine-protein kinase 5; serine/threonine-protein kinase 12; serine/threonine-protein kinase aurora-B; protein phosphatase 1, regulatory subunit 48; aurora- and Ipl1-like midbody-associated protein 1
 UniProt Protein Name    Aurora kinase B
 UniProt Synonym Protein Names   
Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B
 UniProt Gene Name    AURKB [Similar Products]
 UniProt Synonym Gene Names    AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2 [Similar Products]
 UniProt Entry Name    AURKB_HUMAN
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 NCBI Summary for AURORA B    This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
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 UniProt Comments for AURORA B    Function: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis. A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.29 Ref.31 Ref.42 Ref.43

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB. Positive feedback between GSG2 and AURKB contributes to CPC localization. Ref.20 Ref.23 Ref.25

Subunit structure: Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1 and TACC1. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-232-phosphorylated AURKB form during prometaphase and metaphase. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Interacts with TTC28. Ref.12 Ref.17 Ref.20 Ref.21 Ref.23 Ref.25 Ref.26 Ref.27 Ref.28 Ref.30 Ref.34 Ref.38 Ref.39 Ref.43 Ref.44 Ref.45

Subcellular location: Nucleus. Chromosome. Chromosome › centromere. Cytoplasm › cytoskeleton › spindle. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Ref.12 Ref.13 Ref.18 Ref.20 Ref.28 Ref.38 Ref.43 Ref.45

Tissue specificity: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Ref.2 Ref.3

Induction: Expression is cell cycle-regulated, with a low in G1/S, an increase during G2 and M. Expression decreases again after M phase. Ref.20 Ref.23 Ref.25

Post-translational modification: The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Ref.32 Ref.36

Involvement in disease: Disruptive regulation of expression is a possible mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence AAH13300.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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Product References and Citations for AURORA B recombinant protein

   1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.
2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004.
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 Research Articles on AURORA B    1. Data indicate that Aurora-B overexpression may contribute to malignant potential and increased aneuploidy in non-small-cell lung cancer (NSCLC) and may serve as a new therapeutic target in against NSCLC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AURORA B recombinant proteinPathways associated with AURORA B recombinant protein
 Reference Product  PubMed Publications
 BIRC5 recombinant protein  >5 publications with AURORA B and BIRC5
 BUB1B recombinant protein  >3 publications with AURORA B and BUB1B
 KIF2C recombinant protein  >3 publications with AURORA B and KIF2C
 INCENP recombinant protein  >3 publications with AURORA B and INCENP
 CDC20 recombinant protein  >2 publications with AURORA B and CDC20
 CENPA recombinant protein  >2 publications with AURORA B and CENPA
 CDCA8 recombinant protein  >2 publications with AURORA B and CDCA8
 RACGAP1 recombinant protein  >1 publications with AURORA B and RACGAP1
 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Aurora C Signaling Pathway antibodies  Aurora C Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
Diseases associated with AURORA B recombinant proteinOrgans/Tissues associated with AURORA B recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >48 publications with AURORA B and Neoplasms
 Carcinoma Antibodies  >16 publications with AURORA B and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >7 publications with AURORA B and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >6 publications with AURORA B and Breast Neoplasms
 Lung Neoplasms Antibodies  >5 publications with AURORA B and Lung Neoplasms
 Nervous System Diseases Antibodies  >5 publications with AURORA B and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with AURORA B and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >3 publications with AURORA B and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with AURORA B and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >3 publications with AURORA B and Prostatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with AURORA B and Blood
 Prostate Antibodies  >6 publications with AURORA B and Prostate
 Bone Marrow Antibodies  >6 publications with AURORA B and Bone Marrow
 Lung Antibodies  >6 publications with AURORA B and Lung
 Bone Antibodies  >6 publications with AURORA B and Bone
 Brain Antibodies  >5 publications with AURORA B and Brain
 Liver Antibodies  >4 publications with AURORA B and Liver
 Skin Antibodies  >2 publications with AURORA B and Skin
 Ovary Antibodies  >2 publications with AURORA B and Ovary
 Embryonic Tissue Antibodies  >2 publications with AURORA B and Embryonic Tissue
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