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aFGF recombinant protein :: Acidic Fibroblast Growth Factor Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203092
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acidic Fibroblast Growth Factor (aFGF), Recombinant Protein

★Popular Item★
 Also Known As   

Acidic Fibroblast Growth Factor(aFGF), Human, Recombinant, E Coli

 Product Synonym Names    FGF1; FGFA; Acidic Fibroblast Growth Factor (aFGF); HBGF-1; Acidic fibroblast growth factor; aFGF; Beta-endothelial cell growth factor; ECGF-beta; Heparin-binding growth factor 1; Acidic Fibroblast Growth Factor Acidic fibroblast growth factor; AFGF; Beta endothelial cell growth factor; ECGF; ECGF beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fibroblast growth factor 1; Fibroblast Growth Factor 1 Acidic; GLIO703; HBGF 1; HBGF1; Heparin binding growth factor 1; Heparin binding growth factor 1 precursor.
 Product Gene Name   

aFGF recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
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 Purity/Purification    > 95% by SDS - PAGE
 Form/Format    Liquid. 20 mM Tris, pH 7.0, 100 mM NaCl
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Expression System    E Coli
 Bioactivity    The ED50 for this effect is < 2.9 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 340,000 units/ml).
 Assay    1. Cell Line: NIH-3T3 (Mus mosculus/ embryo)
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay Medium: serum free RPMI 1640
4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul)
5. Serum Free Starvation: 24hr with RPMI1640
6. Incubation Time: 24 hr (after sample treatment)
7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)
8. Detection method: Brdu assay
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of aFGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for aFGF recombinant protein

   Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.
 Product Categories/Family for aFGF recombinant protein    Hormones & Cytokines
 Applications Tested/Suitable for aFGF recombinant protein   

SDS-PAGE

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 SDS-PAGE of aFGF recombinant protein    aFGF recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.
 NCBI GI #    4503697
 NCBI GeneID    2246
 NCBI Accession #    NP_000791 [Other Products]
 NCBI GenBank Nucleotide #    NM_000800.4 [Other Products]
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 UniProt Secondary Accession #    P07502; Q16588; B2R5T0; D3DQF2 [Other Products]
 UniProt Related Accession #    P05230 [Other Products]
 Molecular Weight    15.9kDa (141aa), confirmed by MALDI-TOF.
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 NCBI Official Full Name    fibroblast growth factor 1 isoform 1
 NCBI Official Synonym Full Names    fibroblast growth factor 1 (acidic)
 NCBI Official Symbol    FGF1 [Similar Products]
 NCBI Official Synonym Symbols   
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; heparin-binding growth factor 1
 UniProt Protein Name    Fibroblast growth factor 1
 UniProt Synonym Protein Names   
Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1
 Protein Family    Acidic fibroblast growth factor intracellular-binding protein
 UniProt Gene Name    FGF1 [Similar Products]
 UniProt Synonym Gene Names    FGFA; FGF-1; aFGF; ECGF; HBGF-1 [Similar Products]
 UniProt Entry Name    FGF1_HUMAN
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 NCBI Summary for aFGF    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
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 UniProt Comments for aFGF    FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; nucleolus; extracellular region; cell cortex; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of cell division; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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Product References and Citations for aFGF recombinant protein

   Jaye M. et al., (1986) Science. 233, 541-544.
Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129.
Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. u.S.A. 97, 49-54.
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 Research Articles on aFGF    1. FGF1 gene polymorphism is associated with a lower risk of developing cleft palate or cleft lip in Iranian patients. clet palatot cleft lip
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with aFGF recombinant proteinPathways associated with aFGF recombinant protein
 Reference Product  PubMed Publications
 FGFR1 recombinant protein  >221 publications with aFGF and FGFR1
 FGFR2 recombinant protein  >123 publications with aFGF and FGFR2
 FGFR3 recombinant protein  >67 publications with aFGF and FGFR3
 FGFR4 recombinant protein  >56 publications with aFGF and FGFR4
 S100A13 recombinant protein  >31 publications with aFGF and S100A13
 SYT1 recombinant protein  >15 publications with aFGF and SYT1
 EGFR recombinant protein  >14 publications with aFGF and EGFR
 GRB2 recombinant protein  >11 publications with aFGF and GRB2
 FGF23 recombinant protein  >7 publications with aFGF and FGF23
 FGFBP1 recombinant protein  >5 publications with aFGF and FGFBP1
 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
Diseases associated with aFGF recombinant proteinOrgans/Tissues associated with aFGF recombinant protein
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >137 publications with aFGF and Nervous System Diseases
 Heart Diseases Antibodies  >63 publications with aFGF and Heart Diseases
 Inflammation Antibodies  >60 publications with aFGF and Inflammation
 Necrosis Antibodies  >59 publications with aFGF and Necrosis
 Myocardial Ischemia Antibodies  >55 publications with aFGF and Myocardial Ischemia
 Neoplasms, Experimental Antibodies  >43 publications with aFGF and Neoplasms, Experimental
 Hyperplasia Antibodies  >39 publications with aFGF and Hyperplasia
 Fibrosis Antibodies  >35 publications with aFGF and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >33 publications with aFGF and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >33 publications with aFGF and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >300 publications with aFGF and Brain
 Muscle Antibodies  >248 publications with aFGF and Muscle
 Nerve Antibodies  >193 publications with aFGF and Nerve
 Embryonic Tissue Antibodies  >168 publications with aFGF and Embryonic Tissue
 Bone Antibodies  >148 publications with aFGF and Bone
 Heart Antibodies  >119 publications with aFGF and Heart
 Liver Antibodies  >116 publications with aFGF and Liver
 Lung Antibodies  >115 publications with aFGF and Lung
 Eye Antibodies  >106 publications with aFGF and Eye
 Skin Antibodies  >83 publications with aFGF and Skin
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