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Product Name
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Adiponectin Trimeric Form (mAcrp30), Recombinant Protein ★Popular Item★
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Also Known As
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Recombinant Mouse Adiponectin Trimeric Form
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Product Synonym Names
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Acrp30 Mouse, Trimeric; Adiponectin Mouse Recombinant, Trimeric form; Acrp30; AdipoQ; GBP-28; APM-1; ACDC; mAcrp30 Tri
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Product Gene Name
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[Similar Products]
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Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
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ModBase 3D Structure for Q60994
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Host
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HEK293 (Human embryonic kidney cell line)
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Purity/Purification
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Acrp30 Mouse purity is greater than 95% as determined by SDS-PAGE.
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Form/Format
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Mouse Acrp30 filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2. Filtered white lyophilized (freeze-dried) powder.
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Patent Rights
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The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).
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Solubility
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Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
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Preparation and Storage
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Store lyophilized Acrp30 Mouse at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Mouse can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
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Other Notes
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Small volumes of mAcrp30 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Description: Trimeric form of Acrp30 Mouse was expressed in HEK293 cells.The cysteine 39 was replaced with alanine (C39A) 9. mAd-C39A can only form trimer, but not hexamer or HMW form.
Introduction: Adiponectin is a hormone exclusively expressed from adipose tissue. Many studies demonstrate that adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle, attenuate hepatic lipogenesis and luconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells.In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (MMW, also called trimer) forms 8. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.
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Product Categories/Family for mAcrp30 recombinant protein
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CYTOKINES AND GROWTH FACTORS; Cytokines; Adiponectin
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NCBI/Uniprot data below describe general gene information for mAcrp30. It may not necessarily be applicable to this product. |
NCBI GI #
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87252711
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NCBI GeneID
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11450
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NCBI Accession #
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NP_033735.3
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[Other Products]
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NCBI GenBank Nucleotide #
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NM_009605.4
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[Other Products]
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UniProt Primary Accession #
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Q60994
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[Other Products]
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UniProt Secondary Accession #
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Q62400; Q6GTX4; Q9DC68
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[Other Products]
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UniProt Related Accession #
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Q60994
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[Other Products]
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Molecular Weight
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26,809 Da
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NCBI Official Full Name
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adiponectin
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NCBI Official Synonym Full Names
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adiponectin, C1Q and collagen domain containing
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NCBI Official Symbol
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Adipoq
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[Similar Products]
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NCBI Official Synonym Symbols
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Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
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[Similar Products]
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NCBI Protein Information
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adiponectin; 30 kDa adipocyte complement-related protein; adipocyte complement related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adipocyte-specific protein AdipoQ
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UniProt Protein Name
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Adiponectin
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UniProt Synonym Protein Names
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30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ
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UniProt Gene Name
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Adipoq
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[Similar Products]
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UniProt Synonym Gene Names
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Acdc; Acrp30; Apm1; ACRP30
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[Similar Products]
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UniProt Entry Name
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ADIPO_MOUSE
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UniProt Comments for mAcrp30
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adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.
Protein type: Secreted; Secreted, signal peptide; Endoplasmic reticulum; Hormone
Cellular Component: extracellular space; protein complex; cell surface; collagen; endoplasmic reticulum; extracellular region
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; hormone activity; sialic acid binding; receptor binding
Biological Process: negative regulation of phagocytosis; negative regulation of MAP kinase activity; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; positive regulation of cellular protein metabolic process; membrane hyperpolarization; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of signal transduction; negative regulation of heterotypic cell-cell adhesion; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; fatty acid beta-oxidation; positive regulation of fatty acid metabolic process; cellular response to insulin stimulus; negative regulation of macrophage differentiation; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of protein kinase activity; negative regulation of inflammatory response; brown fat cell differentiation; fatty acid oxidation; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure
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Research Articles on mAcrp30
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1. documented that either adiponectin or the synthetic peptide with adiponectin properties, ADP355, suppressed p-FAK in synthetic matrices with stiffness measurements of 9 and 15 kPa
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Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with mAcrp30 recombinant protein | Diseases associated with mAcrp30 recombinant protein |
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Organs/Tissues associated with mAcrp30 recombinant protein |
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