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ACAN recombinant protein :: Aggrecan, Interglobular Domain Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS637420 ACAN recombinant protein
Unit / Price
0.05 mg  /  $685 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aggrecan, Interglobular Domain (ACAN), Recombinant Protein

 Also Known As   

Aggrecan, Recombinant, Interglobular Domain (Aggrecan IgD1, Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, L

 Product Synonym Names    Aggrecan, Recombinant, Interglobular Domain (Aggrecan IgD1, Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, L
 Product Gene Name   

ACAN recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 15; NC_000015.9 (89346674..89418585). Location: 15q26.1
 OMIM    155760
 3D Structure    ModBase 3D Structure for P16112
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 Purity/Purification    Highly Purified
Recombinant aggrecan interglobular domain appears as a major band at about 21kD in SDS-PAGE, representing 90% of total protein in the preparation.
 Form/Format    Supplied as a liquid in 50mM Tris HCI, pH 7.5, 150mM sodium chloride, 5mM calcium chloride.
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 Preparation and Storage    For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
 Other Notes    Small volumes of ACAN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for ACAN recombinant protein

   Aggrecan is a large aggregating proteoglycan of articular cartilage. It is also found in aorta, discs and tendons (1,2). The aggrecan core protein consists of 2317aa (2). Up to 130 glucosaminoglycan chains are attached to the core protein. The total molecular mass can reach 2.2-3 x 10e3kD (4). Within the aggrecan molecule, 3 global domains G1, G2 and G3 can be distinguished. Domains G1 and G2 are connected by a rod-shaped polypeptide called interglobular domain (IGD). The sequence between domains G2 and G3 contains attachment regions for keratan sulfate and chondroitin sulfate chains. Aggrecan interacts via the G1domain with hyaluronan and link protein to form large aggregates. Such aggregates can contain up to 50-100 aggrecan monomers noncovalently bound to a single hyaluronan chain through 2 link proteins (1,2,4). The aggregates form a hydrated gel-like structurem, which endowes cartilage with resistibility to compression and deformation. Degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging (5). Isolated aggrecanases cleave aggrecan at 4 sites within the chrondroitin sulfate-rich region (sites E1667-G1668, E1480-G1481, E1771-A1772, E1871-L1872) and 1 site within the interglobular domain (E373-A374) (6). Cleavage at the latter site had been documented by analysis of cartilage proteoglycan breakdown products in rheumatoid and osteoarthritis (7).

To measure aggrecanase activity, an artificial recombinant protein composed of aggrecan interglobular domain with flanking FLAG-sequence and human immunoglobulin G1 constant region was first used by Hughes et al. (8).
 Product Categories/Family for ACAN recombinant protein    Molecular Biology; MB-Carbohydrates, Glycoproteins
 Applications Tested/Suitable for ACAN recombinant protein   

ELISA (EL/EIA)

 Application Notes for ACAN recombinant protein    Aggrecan interglobular domain is used as substrate for aggrecanases and matrix metalloproteinases. For proteinase activity measurements, the protein is incubated with proteinase for various time intervals. Thereafter, aliquots of the incubation mixture are analyzed by SDS-PAGE or by ELISA. Upon cleavage with aggrecanases the apparent Mr of aggrecan interlobular domain in SDS-PAGE is reduced from 21kD to ~13kD. Quantitative measurement of aggrecanase cleavage requires a neoepitope antibody with specificity for the N-terminus ARGSVILT... appearing upon hydrolysis. The fragment with the newly formed N-terminus is fixed by the neoepitope antibody to a microplate and quantified with an anti-His-tag antibody. In analogy, cleavage by matrix metalloproteinases can be measured with antibodies to neoepitopes appearing upon action of these enzymes.
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NCBI/Uniprot data below describe general gene information for ACAN. It may not necessarily be applicable to this product.
 NCBI GI #    256017257
 NCBI GeneID    176
 NCBI Accession #    NP_037359.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_013227.3 [Other Products]
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 UniProt Primary Accession #    P16112 [Other Products]
 UniProt Secondary Accession #    Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0 [Other Products]
 UniProt Related Accession #    P16112 [Other Products]
 Molecular Weight    15.493kD [Similar Products]
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 NCBI Official Full Name    aggrecan core protein isoform 2
 NCBI Official Synonym Full Names    aggrecan
 NCBI Official Symbol    ACAN [Similar Products]
 NCBI Official Synonym Symbols   
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
[Similar Products]
 NCBI Protein Information    aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
 UniProt Protein Name    Aggrecan core protein
 UniProt Synonym Protein Names   
Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1
 Protein Family    Aggrecan core protein
 UniProt Gene Name    ACAN [Similar Products]
 UniProt Synonym Gene Names    AGC1; CSPG1; MSK16; CSPCP [Similar Products]
 UniProt Entry Name    PGCA_HUMAN
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 NCBI Summary for ACAN    This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACAN    ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function: protein binding; extracellular matrix structural constituent; metal ion binding; hyaluronic acid binding; carbohydrate binding

Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process; proteolysis; cell adhesion; skeletal development

Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type
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 Research Articles on ACAN    1. The total mole fraction of unelongated xylose residues per aggrecan was significantly less (p = 0.03) after IL-1beta treatment compared to control cultures.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACAN recombinant proteinPathways associated with ACAN recombinant protein
 Reference Product  PubMed Publications
 SOX9 recombinant protein  >42 publications with ACAN and SOX9
 ADAMTS4 recombinant protein  >7 publications with ACAN and ADAMTS4
 BMP2 recombinant protein  >4 publications with ACAN and BMP2
 FMOD recombinant protein  >3 publications with ACAN and FMOD
 LUM recombinant protein  >2 publications with ACAN and LUM
 KERA recombinant protein  >1 publications with ACAN and KERA
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram
 Keratan Sulfate Degradation Pathway antibodies  Keratan Sulfate Degradation Pathway Diagram
 Keratan Sulfate/keratin Metabolism Pathway antibodies  Keratan Sulfate/keratin Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
Organs/Tissues associated with ACAN recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >21 publications with ACAN and Connective Tissue
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