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APP recombinant protein :: Amyloid Precursor Protein (APP) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2010207 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment) APP recombinant protein
Unit / Price
0.01 mg  /  $200 +1 FREE 8GB USB
0.05 mg  /  $430 +1 FREE 8GB USB
0.1 mg  /  $665 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Amyloid Precursor Protein (APP), Recombinant Protein

 Also Known As   

Recombinant Amyloid Precursor Protein (APP)

 Product Gene Name   

APP recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S-LEV PTDGNAGLLA EPQIAMFCGK LNMHMNVQNG KWESDPSGTK TCIGTKEGIL QYCQEVYPEL QITNVVEANQ PVTIQNWCKR GRKQCKTHTH IVIPYRCLVG EFVSDALLVP DKCKFLHQER MDVCETHLHW HTVAKETCSE KSTNLHDYGM LLPCGIDKFR GVEFVCCPLA EESDSVDSAD AEEDDSDVWW GGADTDYADG GEDKVVEVAE EEEVADVEEE EADDDEDVED GDEVEEEAEE PYEEATERTT STATTTTTTT ESVEEV
 Chromosome Location    Chromosome: 16; NC_000082.6 (84954436..85173952, complement). Location: 16 C3-qter; 16 46.92 cM
 3D Structure    ModBase 3D Structure for P12023
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Leu18~Val286 (Accession # P12023) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of APP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for APP recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for APP recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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NCBI/Uniprot data below describe general gene information for APP. It may not necessarily be applicable to this product.
 NCBI GI #    311893401
 NCBI GeneID    11820
 NCBI Accession #    NP_001185752.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001198823.1 [Other Products]
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 UniProt Primary Accession #    P12023 [Other Products]
 UniProt Secondary Accession #    P97487; P97942; Q99K32 [Other Products]
 UniProt Related Accession #    P12023 [Other Products]
 Molecular Weight    31.2kDa
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 NCBI Official Full Name    amyloid beta A4 protein isoform 1
 NCBI Official Synonym Full Names    amyloid beta (A4) precursor protein
 NCBI Official Symbol    App [Similar Products]
 NCBI Official Synonym Symbols   
Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
[Similar Products]
 NCBI Protein Information    amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog
 UniProt Protein Name    Amyloid beta A4 protein
 UniProt Synonym Protein Names   
ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99; Alternative name(s):; APP-C99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; APP-C59; Amyloid intracellular domain 59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; APP-C57; Amyloid intracellular domain 57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AID(50); Gamma-CTF(50)
 Protein Family    Amyloid beta A4 protein
 UniProt Gene Name    App [Similar Products]
 UniProt Synonym Gene Names    APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50) [Similar Products]
 UniProt Entry Name    A4_MOUSE
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 UniProt Comments for APP    APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface; Apoptosis; Transcription factor

Cellular Component: Golgi apparatus; extracellular space; smooth endoplasmic reticulum; cell surface; integral to membrane; terminal button; coated pit; intercellular junction; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; membrane; perinuclear region of cytoplasm; nuclear envelope lumen; cytoplasm; intracellular; cytoplasmic vesicle; neuromuscular junction; receptor complex; endosome; neuron projection; intracellular membrane-bound organelle; cell; axon; apical part of cell; plasma membrane; spindle midzone

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; identical protein binding; protease activator activity; protease inhibitor activity; metal ion binding; PTB domain binding; acetylcholine receptor binding; protein binding; enzyme binding; DNA binding; transition metal ion binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; apoptosis; adult locomotory behavior; negative regulation of peptidase activity; mRNA polyadenylation; locomotory behavior; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; collateral sprouting in the absence of injury; visual learning; neuromuscular process controlling balance; cell adhesion; neurite development; protein homooligomerization; nervous system development; cholesterol metabolic process; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; axon cargo transport; mating behavior; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; negative regulation of neuron differentiation; axonogenesis; suckling behavior; ionotropic glutamate receptor signaling pathway; regulation of gene expression; regulation of synapse structure and activity; regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; neuron remodeling
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 Research Articles on APP    1. the role for Abeta in differentiation and perhaps tumor suppression may lie partly in its ability to negatively regulate Akt.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with APP recombinant proteinDiseases associated with APP recombinant protein
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4890 publications with APP and Nervous System Diseases
 Dementia Antibodies  >4813 publications with APP and Dementia
 Brain Diseases Antibodies  >4675 publications with APP and Brain Diseases
 Alzheimer Disease Antibodies  >4611 publications with APP and Alzheimer Disease
 Neurodegenerative Diseases Antibodies  >4295 publications with APP and Neurodegenerative Diseases
 Disease Models, Animal Antibodies  >1489 publications with APP and Disease Models, Animal
 Plaque, Amyloid Antibodies  >1075 publications with APP and Plaque, Amyloid
 Neoplasms Antibodies  >777 publications with APP and Neoplasms
 Inflammation Antibodies  >547 publications with APP and Inflammation
 Cardiovascular Diseases Antibodies  >487 publications with APP and Cardiovascular Diseases
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