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ARTN recombinant protein :: Artemin (ARTN) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2009521 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $225 +1 FREE 8GB USB
0.1 mg  /  $340 +1 FREE 8GB USB
0.2 mg  /  $415 +1 FREE 8GB USB
0.5 mg  /  $800 +1 FREE 8GB USB
1 mg  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Artemin (ARTN), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Artemin (ARTN)

 Product Gene Name   

ARTN recombinant protein

[Similar Products]
 Matching Pairs    Antibody: Artemin (ARTN) (MBS2006251)
Antigen: Artemin (ARTN) (MBS2009521)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-AGTRSS RAR TTDARGCRLR SQLVPVSALG LGHSSDELIR FRFCSGSCRR ARSQHDLSLA SLLGAGALRS PPGSRPISQP CCRPTRYEAV SFMDVNSTWR TVDHLSATAC GCLG
 Chromosome Location    Chromosome: 4; NC_000070.6 (117926160..117930458, complement). Location: 4; 4 D1
 3D Structure    ModBase 3D Structure for Q9Z0L2
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Ala112~Gly224 (Accession # Q9Z0L2) with N-terminal His-Tag
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of ARTN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ARTN recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ARTN recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ARTN recombinant protein    ARTN recombinant protein SDS-PAGE image
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 SDS-PAGE of ARTN recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kOa to 70kOa.
Protein bands: 10kOa, 14kOa, 18kOa, 22kOa, 26kOa, 33kOa, 44kOa and 70kOa.
Double intensity bands: The 26kOa, 18kOa, 10kOa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EOTA, 2% (w/v) SOS, 100mM OTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SOS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SOS-polyacrylamide gel.
[ LOADING VOLUMES]
3-5iJL per well for mini gel; 7iJL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of :55% and minimal band broadening, are confirmed by migration in SOS-PAGE system. Electrophoresis of the Marker on a 13-18% Tris-glycine SOS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of -5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13-18%) large proteins (22-70kDa) can separate, while in low percentage gels (4-8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>1 OOkDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20C, 1 year at -20C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
ARTN recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ARTN. It may not necessarily be applicable to this product.
 NCBI GI #    545746298
 NCBI GeneID    11876
 NCBI Accession #    NP_001271120.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001284191.1 [Other Products]
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 UniProt Primary Accession #    Q9Z0L2 [Other Products]
 UniProt Secondary Accession #    Q3SXF4; Q3SXF5 [Other Products]
 UniProt Related Accession #    Q9Z0L2 [Other Products]
 Molecular Weight    13.6kDa
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 NCBI Official Full Name    artemin isoform 1
 NCBI Official Synonym Full Names    artemin
 NCBI Official Symbol    Artn [Similar Products]
 NCBI Official Synonym Symbols   
neublastin
[Similar Products]
 NCBI Protein Information    artemin
 UniProt Protein Name    Artemin
 UniProt Gene Name    Artn [Similar Products]
 UniProt Entry Name    ARTN_MOUSE
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 UniProt Comments for ARTN    artemin: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Belongs to the TGF-beta family. GDNF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: glial cell line-derived neurotrophic factor receptor binding; protein binding; growth factor activity; receptor binding

Biological Process: axon guidance; cell surface receptor linked signal transduction; peripheral nervous system development; induction of positive chemotaxis
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 Research Articles on ARTN    1. We found that artemin, the specific GFRalpha3 ligand that evokes heat hyperalgesia, robustly sensitized cold responses in a TRPM8-dependent manner in mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ARTN recombinant proteinDiseases associated with ARTN recombinant protein
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 NCAM Signaling For Neurite Out-growth Pathway antibodies  NCAM Signaling For Neurite Out-growth Pathway Diagram
 NCAM1 Interactions Pathway antibodies  NCAM1 Interactions Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >25 publications with ARTN and Nervous System Diseases
 Pain Antibodies  >21 publications with ARTN and Pain
 Neoplasms Antibodies  >17 publications with ARTN and Neoplasms
 Carcinoma Antibodies  >14 publications with ARTN and Carcinoma
 Breast Neoplasms Antibodies  >6 publications with ARTN and Breast Neoplasms
 Adenocarcinoma Antibodies  >5 publications with ARTN and Adenocarcinoma
 Pancreatic Neoplasms Antibodies  >4 publications with ARTN and Pancreatic Neoplasms
 Lung Neoplasms Antibodies  >3 publications with ARTN and Lung Neoplasms
 Hypertrophy Antibodies  >3 publications with ARTN and Hypertrophy
 Inflammation Antibodies  >3 publications with ARTN and Inflammation
Organs/Tissues associated with ARTN recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with ARTN and Brain
 Lung Antibodies  >4 publications with ARTN and Lung
 Tongue Antibodies  >2 publications with ARTN and Tongue
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