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| Product Name
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Aurora kinase A (AURKA), Recombinant Protein
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| Also Known As
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Aurora kinase A, 1-403aa, Human, His tag, E Coli
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| Product Synonym Names
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Serine/threonine-protein kinase 6; Aurora-A; AIK; ARK1; AuRA; BTAK; STK15; STK6; Aurora kinase A; AuRKA; Serine/threonine-protein kinase 6 Aurora 2; Aurora A; aurora kinase A; IAK; Serine/threonine kinase 15.
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Sequence
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MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS
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| OMIM
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D84212 mRNA
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| 3D Structure
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ModBase 3D Structure for O14965
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| Host
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E Coli
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| Purity/Purification
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> 95% by SDS-PAGE
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| Form/Format
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Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.5mM DTT, 100mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF, 20% glycerol.
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| Concentration
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0.5 mg/ml (determined by Bradford assay) (lot specific)
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| Antigen Species
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Human
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| Tag Information
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His-tag
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| Preparation and Storage
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Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
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| Other Notes
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Small volumes of AURKA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Aurora Kinase A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora kinase A is highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also this protein plays a crucial role in tumourigenesis and is overexpressed in many kinds of cancers. Recombinant human Aurora-A fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
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| Product Categories/Family for AURKA recombinant protein
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Cell Cycle
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| Applications Tested/Suitable for AURKA recombinant protein
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SDS-PAGE
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| SDS-Page of AURKA recombinant protein
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TOP
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| NCBI/Uniprot data below describe general gene information for AURKA. It may not necessarily be applicable to this product. |
| NCBI GI #
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38327562
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| NCBI GeneID
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6790
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| NCBI Accession #
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NP_003591
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_003600.3
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[Other Products]
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| UniProt Primary Accession #
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O14965
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[Other Products]
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| UniProt Secondary Accession #
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O60445; O75873; Q9BQD6; Q9UPG5; E1P5F9
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[Other Products]
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| UniProt Related Accession #
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O14965
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[Other Products]
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| Molecular Weight
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47.9 kDa (423aa)
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[Similar Products]
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TOP
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| NCBI Official Full Name
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aurora kinase A
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| NCBI Official Synonym Full Names
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aurora kinase A
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| NCBI Official Symbol
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AURKA
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[Similar Products]
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| NCBI Official Synonym Symbols
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AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
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[Similar Products]
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| NCBI Protein Information
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aurora kinase A
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| UniProt Protein Name
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Aurora kinase A
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| UniProt Synonym Protein Names
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Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A
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| Protein Family
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Aurora kinase
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| UniProt Gene Name
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AURKA
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[Similar Products]
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| UniProt Synonym Gene Names
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AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1
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[Similar Products]
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| NCBI Summary for AURKA
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The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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| UniProt Comments for AURKA
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Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
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TOP
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Tchatchou S., et al. (2007). Cancer lett. 247 (2): 266-72; Chen SS., et al. (2002). EMBO J. 21 (17): 4491-9;
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| Research Articles on AURKA
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1. SIX3 is a novel negative transcriptional regulator and acts as a tumor suppressor that directly represses the transcription of AURKA and AURKB in astrocytoma.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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TOP
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| Products associated with AURKA recombinant protein | Pathways associated with AURKA recombinant protein |
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| Diseases associated with AURKA recombinant protein | Organs/Tissues associated with AURKA recombinant protein |
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TOP
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Recombinant Protein 
