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BX recombinant protein :: Batroxobin Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS143395 BX recombinant protein
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 Product Name   

Batroxobin (BX), Recombinant Protein

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 Also Known As   

Recombinant Batroxobin

 Product Synonym Names    Batroxobin; Batroxobin Recombinant; Thrombin-like enzyme batroxobin; EC 3.4.21.74; BX; Bothrops atrox serine proteinase; Venombin-A; Defibrase; Reptilase; Batroxobin; Batroxobin
 Product Gene Name   

BX recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P
 3D Structure    ModBase 3D Structure for P04971
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 Purity/Purification    Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
 Form/Format    The Batroxobin protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM sodium acetate buffer, pH 7.4.
Sterile Filtered white lyophilized powder.
 Concentration    1mg/ml (lot specific)
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 Source    Pichia Pastoris
 Solubility    It is recommended to reconstitute the lyophilized Batroxobin in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
 Biological Activity    Batroxobin’s biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37°C in vitro within 60 +- 20 seconds.
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 Preparation and Storage    Batroxobin although stable at room temperature for 3 weeks, should be stored below -18 degree C. Upon reconstitution EGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of BX recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for BX recombinant protein

   Description: The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 231 amino acids and having an Mw of approximately 28-33 kDa.

Introduction: Batroxobin is a serin protease that reduces fibronogen levels and is originally extracted from snake venom of Bothrops Atrox. Batroxobin is used in defibrinogenation and thrombolysis and also has an effect on c-fos gene and growth factor.Batroxobin can efficiently restrain proliferation of VSMCs, by blocking the release and uptake of Ca2+, thus influencing [Ca2+]i.Batroxobin is a single chain glycopeptide with a molecular mass of 43kDa on SDS-PAGE gel and its pI-6.6. Batroxobin converts fibrinogen to fibrin through the restricted release of fibrinopeptide-A from fibrinogen to promote blood to clot. Unlike thrombin, it is not affected by heparin and hirudin.
 Product Categories/Family for BX recombinant protein    RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins
NCBI/Uniprot data below describe general gene information for BX. It may not necessarily be applicable to this product.
 NCBI GI #    114837
 NCBI Accession #    P04971.1 [Other Products]
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 UniProt Primary Accession #    P04971 [Other Products]
 Molecular Weight    28,189 Da [Similar Products]
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 NCBI Official Full Name    Thrombin-like enzyme batroxobin
 UniProt Protein Name    Thrombin-like enzyme batroxobin
 UniProt Synonym Protein Names   
Bothrops atrox serine proteinase; Defibrase; Fibrinogen-clotting enzyme; Reptilase; Snake venom serine protease; SVSP; Venombin A
 UniProt Gene Name    BX [Similar Products]
 UniProt Synonym Gene Names    SVTLE; SVSP [Similar Products]
 UniProt Entry Name    VSPF_BOTAT
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 UniProt Comments for BX    Thrombin-like snake venom serine protease. Cleaves Arg-Gly bonds in fibrinogen alpha chains (FGA).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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