View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List C-10 Active Protein Catalog # MBS650853 Unit / Price 0.002 mg / $515 +1 FREE 8GB USB 0.01 mg / $725 +1 FREE 8GB USB 5x0.01 mg / $3,100 +3 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name C-10, Active Protein Popular Item Full Product Name C-10, Recombinant, Mouse (MRP-1, Chemokine C-C motif ligand 6, CCL6) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Host E Coli Purity/Purification Highly Purified97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Form/Format Supplied as a lyophilized powder. No additives. Biological Activity Murine C-10 is fully biologically active when compared to standard. The Biological activity is calculated by the chemotaxis assay using Balb/C mouse spleen MNCs. The maximum chemotactic activity was achieved at 100ng/ml. Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis. Endotoxin Level 0.1ng/ug (IEU/ug) of Murine C-10. Protein Content Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm.2. Analysis by RP-HPLC, using a calibrated solution of Murine C-10 as a Reference Standard. Reconstitution Reconstitute the lyophilized Murine C-10 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Preparation and Storage Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Active Protein Note Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product. Other Notes Small volumes of C-10 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for C-10 active protein Recombinant Murine C-10 is a single, non-glycosylated, polypeptide chain containing 95 amino acids and having a molecular mass of 10710 Dalton.Murine C-10 (MRP-1, Chemokine (C-C motif ligand 6, CCL6) is a member of the C-C chemokine family of proteins related to macrophage inflammatory protein-1 alpha (MIP-1 alpha). Murine C-10 is a 10.7kD protein containing 95 amino acid residues. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1. Product Categories/Family for C-10 active protein Growth Factors; Cytokines; Growth Factors-C-10 NCBI/Uniprot data below describe general gene information for C-10. It may not necessarily be applicable to this product. NCBI GI # 151567668 TOP NCBI Official Full Name Chain C, Ms2-rna Hairpin (c-10) Complex TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP