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mCD14 recombinant protein :: CD14 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS143088 mCD14 recombinant protein
Unit / Price
0.002 mg  /  $265 +1 FREE 8GB USB
0.005 mg  /  $365 +1 FREE 8GB USB
0.01 mg  /  $630 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD14 (mCD14), Recombinant Protein

 Also Known As   

Recombinant Mouse CD14

 Product Synonym Names    CD14 Mouse; CD14 Mouse Recombinant; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14
 Product Gene Name   

mCD14 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P10810
 Host    Chinese Hamster Ovarian Cells (CHO)
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 Form/Format    CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Sterile Filtered White lyophilized (freeze-dried) powder.
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 Solubility    It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
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 Preparation and Storage    Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse CD14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of mCD14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for mCD14 recombinant protein

   Description: The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

Introduction: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
 Product Categories/Family for mCD14 recombinant protein    RECOMBINANT & NATURAL PROTEINS; CD Antigens
 Applications Tested/Suitable for mCD14 recombinant protein   

ELISA (EIA), Inhibition Assays, Western Blot (WB)

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NCBI/Uniprot data below describe general gene information for mCD14. It may not necessarily be applicable to this product.
 NCBI GI #    6753332
 NCBI GeneID    12475
 NCBI Accession #    NP_033971.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_009841.4 [Other Products]
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 UniProt Primary Accession #    P10810 [Other Products]
 UniProt Related Accession #    P10810 [Other Products]
 Molecular Weight    39,204 Da
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 NCBI Official Full Name    monocyte differentiation antigen CD14
 NCBI Official Synonym Full Names    CD14 antigen
 NCBI Official Symbol    Cd14 [Similar Products]
 NCBI Protein Information    monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
 UniProt Protein Name    Monocyte differentiation antigen CD14
 UniProt Synonym Protein Names   
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
 UniProt Gene Name    Cd14 [Similar Products]
 UniProt Entry Name    CD14_MOUSE
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 NCBI Summary for mCD14    This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]
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 UniProt Comments for mCD14    CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion
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 Research Articles on mCD14    1. Data show that ingestion of sCD14 resulted in blood sCD14 levels up.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with mCD14 recombinant proteinDiseases associated with mCD14 recombinant protein
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2321 publications with mCD14 and Inflammation
 Necrosis Antibodies  >2311 publications with mCD14 and Necrosis
 Cardiovascular Diseases Antibodies  >649 publications with mCD14 and Cardiovascular Diseases
 Lung Diseases Antibodies  >531 publications with mCD14 and Lung Diseases
 Liver Diseases Antibodies  >360 publications with mCD14 and Liver Diseases
 Nervous System Diseases Antibodies  >359 publications with mCD14 and Nervous System Diseases
 Disease Models, Animal Antibodies  >330 publications with mCD14 and Disease Models, Animal
 Atherosclerosis Antibodies  >311 publications with mCD14 and Atherosclerosis
 Heart Diseases Antibodies  >280 publications with mCD14 and Heart Diseases
 Asthma Antibodies  >259 publications with mCD14 and Asthma
Organs/Tissues associated with mCD14 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7527 publications with mCD14 and Blood
 Bone Antibodies  >1329 publications with mCD14 and Bone
 Bone Marrow Antibodies  >1069 publications with mCD14 and Bone Marrow
 Liver Antibodies  >624 publications with mCD14 and Liver
 Lung Antibodies  >597 publications with mCD14 and Lung
 Skin Antibodies  >407 publications with mCD14 and Skin
 Kidney Antibodies  >334 publications with mCD14 and Kidney
 Brain Antibodies  >307 publications with mCD14 and Brain
 Muscle Antibodies  >281 publications with mCD14 and Muscle
 Joint Antibodies  >279 publications with mCD14 and Joint
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