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CD154 recombinant protein

Scan QR to view Datasheet Catalog #    MBS232306 CD154 recombinant protein
Unit / Price
0.01 mg  /  $505 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD154, Recombinant Protein

 Also Known As   

RECOMBINANT HUMAN CD154

 Product Gene Name   

CD154 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    300386
 3D Structure    ModBase 3D Structure for P29965
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 Purity/Purification    >98% by SDS PAGE and HPLC sequence analysis
 Form/Format    Purified recombinant protein - lyophilised
 Concentration    Approximate Protein Concentration: 1.0 mg/ml (lot specific)
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 Reconstitution    Reconstitute with 10ul 5-10mM sodium phosphate pH7.5. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Human
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 Preparation and Storage    Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
 Other Notes    Small volumes of CD154 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CD154 recombinant protein

   Recombinant Human CD154 is an E Coli derived protein corresponding to human CD154, also known as CD40 ligand, CD40L or TRAP. CD154 is a membrane protein expressed by activated T cells. Binding of CD154 to its ligand (CD40) on B cells induces B cell proliferation and immunoglobulin class switching.This preparation of Recombinant Human CD154 comprises the receptor binding TNF-like domain of human CD154.
 Applications Tested/Suitable for CD154 recombinant protein   

Functional Assays (FN), Western Blot (WB)

 Application Notes for CD154 recombinant protein    Western Blotting: Minimum Dilution: 0.05 ug; Maximum Dilution: 0.5 ug
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NCBI/Uniprot data below describe general gene information for CD154. It may not necessarily be applicable to this product.
 NCBI GI #    4557433
 NCBI GeneID    959
 NCBI Accession #    NP_000065.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000074.2 [Other Products]
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 UniProt Primary Accession #    P29965 [Other Products]
 UniProt Related Accession #    P29965 [Other Products]
 Molecular Weight    Protein Molecular Weight: 16.3kDa (149 amino acid sequence)
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 NCBI Official Full Name    CD40 ligand
 NCBI Official Synonym Full Names    CD40 ligand
 NCBI Official Symbol    CD40LG [Similar Products]
 NCBI Official Synonym Symbols   
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
[Similar Products]
 NCBI Protein Information    CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
 UniProt Protein Name    CD40 ligand
 UniProt Synonym Protein Names   
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
 UniProt Gene Name    CD40LG [Similar Products]
 UniProt Synonym Gene Names    CD40L; TNFSF5; TRAP; CD40-L; TRAP [Similar Products]
 UniProt Entry Name    CD40L_HUMAN
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 NCBI Summary for CD154    The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CD154    CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1
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 Research Articles on CD154    1. we report results obtained on their effect in suppressing both the TNFa- and the CD40L-induced activation of NF-kappaB in sensor cells that contain an NF-kappaB-inducible SEAP construct.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD154 recombinant proteinPathways associated with CD154 recombinant protein
 Reference Product  PubMed Publications
 CD40 recombinant protein  >29 publications with CD154 and CD40
 ICAM1 recombinant protein  >4 publications with CD154 and ICAM1
 CD86 recombinant protein  >4 publications with CD154 and CD86
 TRAF1 recombinant protein  >2 publications with CD154 and TRAF1
 RELA recombinant protein  >1 publications with CD154 and RELA
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Diseases associated with CD154 recombinant proteinOrgans/Tissues associated with CD154 recombinant protein
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >11 publications with CD154 and Autoimmune Diseases
 Inflammation Antibodies  >10 publications with CD154 and Inflammation
 Necrosis Antibodies  >7 publications with CD154 and Necrosis
 Cardiovascular Diseases Antibodies  >5 publications with CD154 and Cardiovascular Diseases
 Myocardial Infarction Antibodies  >3 publications with CD154 and Myocardial Infarction
 Hyper-IgM Immunodeficiency Syndrome, Type 1 Antibodies  >3 publications with CD154 and Hyper-IgM Immunodeficiency Syndrome, Type 1
 Myocardial Ischemia Antibodies  >3 publications with CD154 and Myocardial Ischemia
 Pneumonia Antibodies  >3 publications with CD154 and Pneumonia
 Anemia Antibodies  >3 publications with CD154 and Anemia
 Heart Diseases Antibodies  >3 publications with CD154 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with CD154 and Blood
 Mixed Antibodies  >1 publications with CD154 and Mixed
 Thymus Antibodies  >1 publications with CD154 and Thymus
 Brain Antibodies  >1 publications with CD154 and Brain
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