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CD2AP recombinant protein :: CD2 Associated Protein (CD2AP) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2018570 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $185 +1 FREE 8GB USB
0.05 mg  /  $385 +1 FREE 8GB USB
0.1 mg  /  $595 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD2 Associated Protein (CD2AP), Recombinant Protein

 Also Known As   

Recombinant CD2 Associated Protein (CD2AP)

 Product Gene Name   

CD2AP recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TL NNKLGLFPSN FVKELEVTDD GETHEAQDDS ETVLAGPTSP IPSLGNVSET ASGSVTQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK PEKPLILQSL GPKTQSVEIT KTDTEGKIKA KEYCRTLFAY EGTNEDELTF KEGEIIHLIS KETGEAGWWR GELNGKEGVF PDNFAVQINE LDKDFPKPKK PPPPAKAPAP KPELIAAEKK YFSLKPEEKD EKSTLEQKPS KPAAPQVPPK KPTPPTKASN LLRSSGTVYP KRPEKPVPPP PPIAKINGE
 Chromosome Location    Chromosome: 6; NC_000006.11 (47445482..47594999). Location: 6p12
 OMIM    604241
 3D Structure    ModBase 3D Structure for Q9Y5K6
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 5% trehalose, and preservative.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Thr149~Glu429 (Accession # Q9Y5K6) with two N-terminal Tags, His-tag and T7-tag
 Subcellular Location    Cytoplasm, cytoskeleton. Cell projection, ruffle.
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
 Predicted isoelectric point    7.8
 Accurate Molecular Mass    44kDa as determined by SDS-PAGE reducing conditions.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of CD2AP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for CD2AP recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for CD2AP recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of CD2AP recombinant protein    CD2AP recombinant protein SDS-PAGE image
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 SDS-PAGE of CD2AP recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
CD2AP recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for CD2AP. It may not necessarily be applicable to this product.
 NCBI GI #    11321634
 NCBI GeneID    23607
 NCBI Accession #    NP_036252.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012120.2 [Other Products]
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 UniProt Primary Accession #    Q9Y5K6 [Other Products]
 UniProt Secondary Accession #    Q5VYA3; Q9UG97; A6NL34 [Other Products]
 UniProt Related Accession #    Q9Y5K6 [Other Products]
 Molecular Weight    34.5kDa
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 NCBI Official Full Name    CD2-associated protein
 NCBI Official Synonym Full Names    CD2-associated protein
 NCBI Official Symbol    CD2AP [Similar Products]
 NCBI Official Synonym Symbols   
CMS
[Similar Products]
 NCBI Protein Information    CD2-associated protein; adapter protein CMS; cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains
 UniProt Protein Name    CD2-associated protein
 UniProt Synonym Protein Names   
Adapter protein CMS; Cas ligand with multiple SH3 domains
 Protein Family    CD2-associated protein
 UniProt Gene Name    CD2AP [Similar Products]
 UniProt Entry Name    CD2AP_HUMAN
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 NCBI Summary for CD2AP    This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CD2AP    CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3).

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: ruffle; filamentous actin; cytoplasm; nucleolus; plasma membrane; intercellular junction; actin cytoskeleton

Molecular Function: protein binding; structural constituent of cytoskeleton; SH3 domain binding

Biological Process: mitosis; cell division; protein complex assembly; signal transduction; substrate-bound cell migration, cell extension; negative regulation of transforming growth factor-beta1 production

Disease: Focal Segmental Glomerulosclerosis 3, Susceptibility To
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 Research Articles on CD2AP    1. an FRS2beta-CIN85/CD2AP-Cbl axis for downregulation of ErbB2 may regulate ErbB2 protein levels in physiological and pathological settings
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD2AP recombinant proteinPathways associated with CD2AP recombinant protein
 Reference Product  PubMed Publications
 CD2 recombinant protein  >148 publications with CD2AP and CD2
 NPHS2 recombinant protein  >58 publications with CD2AP and NPHS2
 CBL recombinant protein  >18 publications with CD2AP and CBL
 FAM125A recombinant protein  >4 publications with CD2AP and FAM125A
 KIRREL recombinant protein  >2 publications with CD2AP and KIRREL
 FYN recombinant protein  >2 publications with CD2AP and FYN
 Products by Pathway  Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
 VEGFR1 Specific Signals Pathway antibodies  VEGFR1 Specific Signals Pathway Diagram
Diseases associated with CD2AP recombinant proteinOrgans/Tissues associated with CD2AP recombinant protein
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >99 publications with CD2AP and Kidney Diseases
 Proteinuria Antibodies  >83 publications with CD2AP and Proteinuria
 Glomerulosclerosis, Focal Segmental Antibodies  >43 publications with CD2AP and Glomerulosclerosis, Focal Segmental
 Nephritis Antibodies  >41 publications with CD2AP and Nephritis
 Nephrosis Antibodies  >33 publications with CD2AP and Nephrosis
 Renal Insufficiency Antibodies  >15 publications with CD2AP and Renal Insufficiency
 Neoplasms Antibodies  >15 publications with CD2AP and Neoplasms
 Alzheimer Disease Antibodies  >15 publications with CD2AP and Alzheimer Disease
 Inflammation Antibodies  >7 publications with CD2AP and Inflammation
 Fibrosis Antibodies  >6 publications with CD2AP and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >151 publications with CD2AP and Kidney
 Blood Antibodies  >35 publications with CD2AP and Blood
 Bone Antibodies  >9 publications with CD2AP and Bone
 Brain Antibodies  >7 publications with CD2AP and Brain
 Embryonic Tissue Antibodies  >6 publications with CD2AP and Embryonic Tissue
 Skin Antibodies  >6 publications with CD2AP and Skin
 Bone Marrow Antibodies  >5 publications with CD2AP and Bone Marrow
 Muscle Antibodies  >4 publications with CD2AP and Muscle
 Eye Antibodies  >4 publications with CD2AP and Eye
 Testis Antibodies  >3 publications with CD2AP and Testis
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