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PTPRC recombinant protein :: CD45 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS635951 PTPRC recombinant protein
Unit / Price
0.05 mg  /  $710 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD45 (PTPRC), Recombinant Protein

★Popular Item★
 Also Known As   

CD45, Recombinant, Human (PTPRC, Protein Tyrosine Phosphatase, Receptor Type C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

 Product Gene Name   

PTPRC recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    Gln-24 to Lys-575 of human CD45 (Accession #P08575) with a C-terminal 6-His tag

Applicati ons:
Su itable for use in ELISA. Other applicatio ns not tested.
 Chromosome Location    Chromosome: 1; NC_000001.10 (198608098..198726605). Location: 1q31-q32
 OMIM    126200
 3D Structure    ModBase 3D Structure for P08575
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 Host    NS0-derived human CD45
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 Purity/Purification    >90%
 Form/Format    Supplied as a lyophilized powder from PBS, pH 7.3. Reconstitute with 500ul sterile PBS
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 Endotoxin    <0.1EU/ug
 Activity    1ug/ml of rhCD45 is captured onto a mouse anti-Polyhistidine antibody coated plate, the concentration of the rhGalectin 1 that produces 50% of the optimal binding is found to be ~0.35-1.4ug/ml
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of PTPRC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PTPRC recombinant protein

   CD45, previously called LCA (leukocyte common antigen) T200, or Ly5inmice, is member C of t he class 1 (receptor­like) protein tyrosine phosphatase f amily (PTPRC). It is avaliably glycosylated 180-220kD transmembrane proteint hat is abundantly expressed on all nucleated cells of hematopoietic origin . CD45 has several isoforms, expressed according t o cell type, developmental stage and anti- genicexposure. The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes. The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronect in type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain t hat contains two hosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is t he shor test form, lackingexons 4,5 and 6 which encode aa32-191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved intoor out of the immunologicalsynapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily O­glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the I S, CD45 ephosphorylates and negatively regulates the Src family kinase, Lck. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokne secretion and cell survival, partially overlapping in function with DEP-1/ CD148. CD45 deletion causes in severe i mmunodeficiency, while pont mutations may be associated with utoimmunedisorders.
 Product Categories/Family for PTPRC recombinant protein    Molecular Biology; MB-CD Markers
 Applications Tested/Suitable for PTPRC recombinant protein   

ELISA

 Application Notes for PTPRC recombinant protein    Optimal dilutions to be determined by the researcher
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NCBI/Uniprot data below describe general gene information for PTPRC. It may not necessarily be applicable to this product.
 NCBI GI #    44242008
 NCBI GeneID    5788
 NCBI Accession #    AAS46946.1 [Other Products]
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 UniProt Primary Accession #    P08575 [Other Products]
 UniProt Secondary Accession #    Q16614; Q9H0Y6; A8K7W6 [Other Products]
 UniProt Related Accession #    P08575 [Other Products]
 Molecular Weight    61.6kD (predicted) [Similar Products]
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 NCBI Official Full Name    CD45
 NCBI Official Synonym Full Names    protein tyrosine phosphatase, receptor type, C
 NCBI Official Symbol    PTPRC [Similar Products]
 NCBI Official Synonym Symbols   
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
[Similar Products]
 NCBI Protein Information    receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
 UniProt Protein Name    Receptor-type tyrosine-protein phosphatase C
 UniProt Synonym Protein Names   
Leukocyte common antigen; L-CA; T200
 Protein Family    Receptor-type tyrosine-protein phosphatase
 UniProt Gene Name    PTPRC [Similar Products]
 UniProt Synonym Gene Names    CD45; L-CA [Similar Products]
 UniProt Entry Name    PTPRC_HUMAN
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 NCBI Summary for PTPRC    The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
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 UniProt Comments for PTPRC    CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive
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 Research Articles on PTPRC    1. This study analysis revealed up-regulation of CD14, CD18, CD45, TLR2, TLR4, CD74, C1q, C3, CCL2, and down-regulation of CD36, CX3CR1, CX3CL1 and CD200 in postmenopausal women.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PTPRC recombinant proteinPathways associated with PTPRC recombinant protein
 Reference Product  PubMed Publications
 CD4 recombinant protein  >2787 publications with PTPRC and CD4
 LCK recombinant protein  >202 publications with PTPRC and LCK
 CD22 recombinant protein  >116 publications with PTPRC and CD22
 CXCR4 recombinant protein  >114 publications with PTPRC and CXCR4
 CD79A recombinant protein  >82 publications with PTPRC and CD79A
 FYN recombinant protein  >76 publications with PTPRC and FYN
 CD79B recombinant protein  >48 publications with PTPRC and CD79B
 LYN recombinant protein  >43 publications with PTPRC and LYN
 CSK recombinant protein  >26 publications with PTPRC and CSK
 SEMA4D recombinant protein  >5 publications with PTPRC and SEMA4D
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
Diseases associated with PTPRC recombinant proteinOrgans/Tissues associated with PTPRC recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1914 publications with PTPRC and Neoplasms
 Lymphoma Antibodies  >847 publications with PTPRC and Lymphoma
 Inflammation Antibodies  >594 publications with PTPRC and Inflammation
 Necrosis Antibodies  >448 publications with PTPRC and Necrosis
 Brain Diseases Antibodies  >284 publications with PTPRC and Brain Diseases
 Lung Diseases Antibodies  >241 publications with PTPRC and Lung Diseases
 Multiple Myeloma Antibodies  >180 publications with PTPRC and Multiple Myeloma
 Neoplasms, Experimental Antibodies  >154 publications with PTPRC and Neoplasms, Experimental
 Arthritis, Rheumatoid Antibodies  >143 publications with PTPRC and Arthritis, Rheumatoid
 Fibrosis Antibodies  >129 publications with PTPRC and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6493 publications with PTPRC and Blood
 Bone Antibodies  >1732 publications with PTPRC and Bone
 Bone Marrow Antibodies  >1622 publications with PTPRC and Bone Marrow
 Vascular Antibodies  >690 publications with PTPRC and Vascular
 Lymph Node Antibodies  >606 publications with PTPRC and Lymph Node
 Lymph Antibodies  >603 publications with PTPRC and Lymph
 Spleen Antibodies  >601 publications with PTPRC and Spleen
 Liver Antibodies  >483 publications with PTPRC and Liver
 Thymus Antibodies  >476 publications with PTPRC and Thymus
 Skin Antibodies  >450 publications with PTPRC and Skin
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