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CD80 recombinant protein :: CD80 (B7-1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS553255 CD80 recombinant protein
Unit / Price
0.02 mg  /  $220 +1 FREE 8GB USB
0.1 mg  /  $595 +1 FREE 8GB USB
0.5 mg  /  $2,220 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD80 (B7-1), Recombinant Protein

★Popular Item★
 Also Known As   

Mouse CD80 Recombinant (B7-1)

 Product Synonym Names    Mouse CD80 Recombinant (B7-1) ; CD80; B7-1
 Product Gene Name   

CD80 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q00609
 Host    HEk293 Cells
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 Purity/Purification    >90%, as determined by SDS-PAGE and HPLC
 Form/Format    Recombinant mouse CD80 was lyophilized from 0.2um filtered 50mM Tris, 100mM NaCl, pH 8.0.
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 Domain    Ig-like C2
 Host Note    Optimized DNA sequence encoding extracellular domain of mouse CD80 including a C-terminal 6His tag was expressed in HEK293 cells.
 Endotoxin    Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
 Molecular Weight Note    Recombinant mouse CD80 is a monomer protein consisting of 220 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.
 Biological Activity    The activity was tested y immobilized recombinant CD80 (1ug/ml) ability to bind recombinant mouse CD28 in functional ELISA.
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 Preparation and Storage    Recombinant mouse CD80, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.

Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of CD80 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for CD80 recombinant protein

   CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig- like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells.One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal.The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a 20-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.
 Product Categories/Family for CD80 recombinant protein    Receptors CD
NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product.
 NCBI GI #    112983684
 NCBI GeneID    12519
 NCBI Accession #    NP_033985.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_009855.2 [Other Products]
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 UniProt Primary Accession #    Q00609 [Other Products]
 UniProt Secondary Accession #    Q61332 [Other Products]
 Molecular Weight    34,590 Da
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 NCBI Official Full Name    T-lymphocyte activation antigen CD80
 UniProt Protein Name    T-lymphocyte activation antigen CD80
 UniProt Synonym Protein Names   
Activation B7-1 antigen
 Protein Family    T-lymphocyte activation antigen
 UniProt Gene Name    Cd80 [Similar Products]
 UniProt Synonym Gene Names    B7; B7 [Similar Products]
 UniProt Entry Name    CD80_MOUSE
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 UniProt Comments for CD80    CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of T cell proliferation; positive regulation of alpha-beta T cell proliferation
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Product References and Citations for CD80 recombinant protein

   tumor cell programmed death ligand 1-mediated t cell suppression is overcome by coexpression of CD80 .J. Immunol., Jun 2011; 186: 6822 - 6829.
toll-like receptor 3 ligands induce CD80 expression in human podocytes via an nf-b-dependent pathway .Nephrol. Dial. Transplant., May 2011; 10.1093/ndt/gfr271.
trans-endocytosis of CD80 and cd86: a molecular basis for the cell-extrinsic function of ctla-4 .Science, Apr 2011; 332: 600 - 603.
critical role of transcription factor pu.1 in the expression of CD80 and cd86 on dendritic cells .Blood, Feb 2011; 117: 2211 - 2222.
enhancement of suboptimal cd8 cytotoxic t cell effector function in vivo using antigen-specific CD80 defective t cells .J. Immunol., Jan 2011; 186: 291 - 304.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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