Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow CDC25A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Claudin 1 (CLDN1) Antibody
 • Neurofilament light polypeptide (NEFL) ELISA Kit
 • Z-DNA Binding Protein 1 (ZBP1) Recombinant Protein
 • Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
 • Catalase ELISA Kit
 • A Disintegrin And Metalloproteinase With Thrombospondin 1 (A... Antibody
 • RNF114 Antibody
 • Isocitrate Dehydrogenase-1 (IDH1 ) Recombinant Protein
 • Glutathione (GSH) Antibody
 • JAG1 Antibody
 • Rituximab (Rituxan) PK ELISA Kit
 • Hemoglobin-Advanced Glycosylation End Products (HB-AGE) ELISA Kit
 • Multidrug resistance-associated protein 1 (ABCC1), partial Recombinant Protein
 • Macrophage Inflammatory Protein 1 Alpha (MIP1a) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CDC25A recombinant protein

Scan QR to view Datasheet Catalog #    MBS203746
SDS-Page
Unit / Price
0.01 mg  /  $310 +1 FREE 8GB USB
0.05 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDC25A, Recombinant Protein

 Also Known As   

CDC25A, 1-524aa, Human, His tag, E Coli

 Product Synonym Names    M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; Cdc 25a; Cell division cycle 25 homolog A (S. pombe); Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform b; D9Ertd393e; EC 3.1.3.48; M phase inducer phosphatase 1; MGC115549
 Product Gene Name   

CDC25A recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQG LGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENE AFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETP SCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKG TIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLK KPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTK SRTWAGEKSK REMYSRLKKL
 OMIM    M81933 mRNA
 3D Structure    ModBase 3D Structure for P30304
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    > 80% by SDS-PAGE
 Form/Format    Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 0.2M NaCl, 1mM EDTA
 Concentration    0.25 mg/ml (determined by Bradford assay) (lot specific)
Section Bar
 Antigen Species    Human
 Tag Information    His-tag
Section Bar
 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of CDC25A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for CDC25A recombinant protein

   M-phase inducer phosphatase 1, also known as CDC25A, is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Recombinant human CDC25A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
 Product Categories/Family for CDC25A recombinant protein    Cell Cycle
 Applications Tested/Suitable for CDC25A recombinant protein   

SDS-PAGE

Section Bar
 SDS-Page of CDC25A recombinant protein    CDC25A recombinant protein SDS-Page image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CDC25A. It may not necessarily be applicable to this product.
 NCBI GI #    42490758
 NCBI GeneID    993
 NCBI Accession #    NP_001780 [Other Products]
 NCBI GenBank Nucleotide #    NM_001789.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P30304 [Other Products]
 UniProt Secondary Accession #    Q8IZH5; Q96IL3; Q9H2F2 [Other Products]
 UniProt Related Accession #    P30304 [Other Products]
 Molecular Weight    63.2 kDa (560aa)
Table BarTOPTable Bar
 NCBI Official Full Name    M-phase inducer phosphatase 1 isoform a
 NCBI Official Synonym Full Names    cell division cycle 25A
 NCBI Official Symbol    CDC25A [Similar Products]
 NCBI Official Synonym Symbols   
CDC25A2
[Similar Products]
 NCBI Protein Information    M-phase inducer phosphatase 1
 UniProt Protein Name    M-phase inducer phosphatase 1
 UniProt Synonym Protein Names   
Dual specificity phosphatase Cdc25A
 Protein Family    M-phase inducer phosphatase
 UniProt Gene Name    CDC25A [Similar Products]
Section Bar
 NCBI Summary for CDC25A    CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CDC25A    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Table BarTOPTable Bar
 

Product References and Citations for CDC25A recombinant protein

   Pereg Y., et al. (2010) Nat. Cell Biol. 12:400-406; Soerensen C.S., et al. (2003) Cancer Cell 3:247-258
Section Bar
 Research Articles on CDC25A    1. MCPH1 interacts with and promotes the E3 ligase betaTrCP2 to degrade Cdc25A independent of DNA damage. Overexpression of betaTrCP2 or the knockdown of Cdc25A remedies the high mitotic index and rescues the premature differentiation of Mcph1-deficient neuroprogenitors in vivo MCPH1 itself is degraded by APC/CCdh1, but not APC/CCdc20, in late mitosis and G1 phase.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CDC25A recombinant proteinPathways associated with CDC25A recombinant protein
 Reference Product  PubMed Publications
 CDK2 recombinant protein  >212 publications with CDC25A and CDK2
 CHEK1 recombinant protein  >107 publications with CDC25A and CHEK1
 CDK4 recombinant protein  >76 publications with CDC25A and CDK4
 CDK1 recombinant protein  >74 publications with CDC25A and CDK1
 CHEK2 recombinant protein  >69 publications with CDC25A and CHEK2
 CCNB1 recombinant protein  >47 publications with CDC25A and CCNB1
 CDK6 recombinant protein  >38 publications with CDC25A and CDK6
 WEE1 recombinant protein  >23 publications with CDC25A and WEE1
 CDC6 recombinant protein  >18 publications with CDC25A and CDC6
 PKMYT1 recombinant protein  >1 publications with CDC25A and PKMYT1
 Products by Pathway  Pathway Diagram
 ATM Signaling Pathway antibodies  ATM Signaling Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram
 Cyclin A:Cdk2-associated Events At S Phase Entry Pathway antibodies  Cyclin A:Cdk2-associated Events At S Phase Entry Pathway Diagram
Diseases associated with CDC25A recombinant proteinOrgans/Tissues associated with CDC25A recombinant protein
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >74 publications with CDC25A and Breast Neoplasms
 Liver Diseases Antibodies  >49 publications with CDC25A and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >44 publications with CDC25A and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >43 publications with CDC25A and Lung Neoplasms
 Liver Neoplasms Antibodies  >43 publications with CDC25A and Liver Neoplasms
 Lung Diseases Antibodies  >39 publications with CDC25A and Lung Diseases
 Carcinoma, Hepatocellular Antibodies  >39 publications with CDC25A and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >32 publications with CDC25A and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >19 publications with CDC25A and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >17 publications with CDC25A and Neoplasm Invasiveness
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >67 publications with CDC25A and Liver
 Lung Antibodies  >64 publications with CDC25A and Lung
 Blood Antibodies  >51 publications with CDC25A and Blood
 Prostate Antibodies  >36 publications with CDC25A and Prostate
 Brain Antibodies  >27 publications with CDC25A and Brain
 Muscle Antibodies  >18 publications with CDC25A and Muscle
 Bone Antibodies  >17 publications with CDC25A and Bone
 Skin Antibodies  >17 publications with CDC25A and Skin
 Kidney Antibodies  >17 publications with CDC25A and Kidney
 Embryonic Tissue Antibodies  >15 publications with CDC25A and Embryonic Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions