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CDC7/DBF4 recombinant protein

Scan QR to view Datasheet Catalog #    MBS515469
Unit / Price
0.005 mg  /  $315 +1 FREE 8GB USB
0.01 mg  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDC7/DBF4, Recombinant Protein

★Popular Item★
 Also Known As   

CDC7/DBF4, Active

 Product Gene Name   

CDC7/DBF4 recombinant protein

[Similar Products]
 Product Synonym Gene Name    CDC7: CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    Full Length
 OMIM    AB003698 mRNA
 3D Structure    ModBase 3D Structure for O00311
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 Host    Sf9 insect cells using baculovirus
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 Purity/Purification    >85%
 Form/Format    Recombinant protein stored in 50mM Tris-HCI,pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1 mM PMSF, 25% Glycerol.
 Concentration    0.1 ug/uL (lot specific)
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 Specific activity    8.5 nmol/min/mg
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 Preparation and Storage    Store product at -70C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Shipping: Ships in dry ice.

Stability:1 year at -70 from date of shipment.
 Other Notes    Small volumes of CDC7/DBF4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CDC7/DBF4 recombinant protein

   Description: Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags.

Scientific Background: CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines (1). CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase (2).
 Product Categories/Family for CDC7/DBF4 recombinant protein    Enzymes; Active Kinases
 Applications Tested/Suitable for CDC7/DBF4 recombinant protein   

Kinase Assay, Western Blot (WB)

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 Testing Data TD of CDC7/DBF4 recombinant protein    CDC7/DBF4 recombinant protein Testing Data TD image
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 Testing Data TD of CDC7/DBF4 recombinant protein    CDC7/DBF4 recombinant protein Testing Data TD image
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NCBI/Uniprot data below describe general gene information for CDC7/DBF4. It may not necessarily be applicable to this product.
 NCBI GI #    197313663
 NCBI GeneID    8317
 NCBI Accession #    NM_003503.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_003503 [Other Products]
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 UniProt Primary Accession #    O00311 [Other Products]
 UniProt Secondary Accession #    O00558; Q5T5U5; D3DT31 [Other Products]
 UniProt Related Accession #    O00311 [Other Products]
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 NCBI Official Full Name    Homo sapiens cell division cycle 7 (CDC7), transcript variant 1, mRNA
 NCBI Official Synonym Full Names    cell division cycle 7
 NCBI Official Symbol    CDC7 [Similar Products]
 NCBI Official Synonym Symbols   
Hsk1; CDC7L1; HsCDC7; huCDC7
[Similar Products]
 NCBI Protein Information    cell division cycle 7-related protein kinase
 UniProt Protein Name    Cell division cycle 7-related protein kinase
 UniProt Gene Name    CDC7 [Similar Products]
 UniProt Synonym Gene Names    CDC7L1; CDC7-related kinase; HsCdc7; huCdc7 [Similar Products]
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 NCBI Summary for CDC7/DBF4    This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
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 UniProt Comments for CDC7/DBF4    CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription.

Protein type: CDC7 family; EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleus

Molecular Function: kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: DNA replication; DNA replication initiation; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; peptidyl-serine phosphorylation; phagocytosis; positive regulation of cell proliferation; regulation of cell shape
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Product References and Citations for CDC7/DBF4 recombinant protein

   1. Hess, G. F. et.al: A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998. 2. Sheu, Y.-J. et.al: The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4. Nature 463: 113-117, 2010.
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 Research Articles on CDC7/DBF4    1. p53-dependent control of CDC7 levels is essential for blocking G1/S cell-cycle transition upon genotoxic stress.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDC7/DBF4 recombinant proteinPathways associated with CDC7/DBF4 recombinant protein
 Reference Product  PubMed Publications
 DBF4 recombinant protein  >151 publications with CDC7/DBF4 and DBF4
 MCM2 recombinant protein  >65 publications with CDC7/DBF4 and MCM2
 CDC45 recombinant protein  >50 publications with CDC7/DBF4 and CDC45
 CDC6 recombinant protein  >42 publications with CDC7/DBF4 and CDC6
 MCM4 recombinant protein  >21 publications with CDC7/DBF4 and MCM4
 MCM5 recombinant protein  >16 publications with CDC7/DBF4 and MCM5
 MCM10 recombinant protein  >9 publications with CDC7/DBF4 and MCM10
 MCM7 recombinant protein  >9 publications with CDC7/DBF4 and MCM7
 MCM3 recombinant protein  >6 publications with CDC7/DBF4 and MCM3
 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
Diseases associated with CDC7/DBF4 recombinant proteinOrgans/Tissues associated with CDC7/DBF4 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >32 publications with CDC7/DBF4 and Neoplasms
 Carcinoma Antibodies  >6 publications with CDC7/DBF4 and Carcinoma
 Skin Neoplasms Antibodies  >3 publications with CDC7/DBF4 and Skin Neoplasms
 Breast Neoplasms Antibodies  >3 publications with CDC7/DBF4 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with CDC7/DBF4 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >2 publications with CDC7/DBF4 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >2 publications with CDC7/DBF4 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with CDC7/DBF4 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >1 publications with CDC7/DBF4 and Pancreatic Neoplasms
 Ovarian Neoplasms Antibodies  >1 publications with CDC7/DBF4 and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >7 publications with CDC7/DBF4 and Eye
 Blood Antibodies  >6 publications with CDC7/DBF4 and Blood
 Skin Antibodies  >3 publications with CDC7/DBF4 and Skin
 Lung Antibodies  >3 publications with CDC7/DBF4 and Lung
 Testis Antibodies  >3 publications with CDC7/DBF4 and Testis
 Bone Marrow Antibodies  >2 publications with CDC7/DBF4 and Bone Marrow
 Kidney Antibodies  >2 publications with CDC7/DBF4 and Kidney
 Heart Antibodies  >2 publications with CDC7/DBF4 and Heart
 Liver Antibodies  >1 publications with CDC7/DBF4 and Liver
 Thyroid Antibodies  >1 publications with CDC7/DBF4 and Thyroid
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