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CDK8 recombinant protein

Scan QR to view Datasheet Catalog #    MBS515108
SDS-PAGE
Unit / Price
0.02 mg  /  $260 +1 FREE 8GB USB
0.05 mg  /  $415 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDK8, Recombinant Protein

★Popular Item★
 Also Known As   

CDK8, Unactive recombinant protein

 Product Synonym Names    K35; MGC126074; MGC126075; CDK8; Unacitve
 Product Gene Name   

CDK8 recombinant protein

[Similar Products]
 Product Synonym Gene Name    K35; MGC126074; MGC126075 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 13; NC_000013.10 (26828262..26979375). Location: 13q12
 OMIM    603184
 3D Structure    ModBase 3D Structure for P49336
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 Host    Sf9 insect cells
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 Purity/Purification    >80%
 Form/Format    Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
 Concentration    0.2ug/ul (lot specific)
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 Type    Recombinant Fusion Protein
 Species    Human
 Tag Information    GST tag
 Expression System    Sf9 insect cells using baculovirus
 Source Note    Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells
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 Preparation and Storage    Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with Dry Ice.
 Other Notes    Small volumes of CDK8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CDK8 recombinant protein

   Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets (2).
 Product Categories/Family for CDK8 recombinant protein    Unactive Kinase; Signaling Proteins - Unactive Kinases
 Applications Tested/Suitable for CDK8 recombinant protein   

Kinase Assay, Western Blot (WB)

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 SDS-PAGE of CDK8 recombinant protein    CDK8 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for CDK8. It may not necessarily be applicable to this product.
 NCBI GI #    47479675
 NCBI GeneID    1024
 NCBI Accession #    BC069634 [Other Products]
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 UniProt Primary Accession #    P49336 [Other Products]
 UniProt Secondary Accession #    Q5VUF3; Q6ISB5 [Other Products]
 UniProt Related Accession #    P49336 [Other Products]
 Molecular Weight    ~84 kDa
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 NCBI Official Full Name    Homo sapiens cyclin-dependent kinase 8, mRNA
 NCBI Official Synonym Full Names    cyclin-dependent kinase 8
 NCBI Official Symbol    CDK8 [Similar Products]
 NCBI Official Synonym Symbols   
K35
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8
 UniProt Protein Name    Cyclin-dependent kinase 8
 UniProt Synonym Protein Names   
Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
 Protein Family    Cyclin-dependent kinase
 UniProt Gene Name    CDK8 [Similar Products]
 UniProt Entry Name    CDK8_HUMAN
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 NCBI Summary for CDK8    The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CDK8    Function: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. Ref.8 Ref.9

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.8ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. Ref.8

Cofactor: Magnesium

By similarity.

Subunit structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II. Interacts with CTNNB1, GLI3 and MAML1. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Nucleus

Probable.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.
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Product References and Citations for CDK8 recombinant protein

   1.Akoulitchev S, et al: TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000.
2.Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.
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 Research Articles on CDK8    1. results show a reverse correlation between CDK8 levels and several key features of the endometrial cancer cells, including cell proliferation, migration and invasion as well as tumor formation in vivo
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDK8 recombinant proteinPathways associated with CDK8 recombinant protein
 Reference Product  PubMed Publications
 CCNC recombinant protein  >20 publications with CDK8 and CCNC
 MED12 recombinant protein  >16 publications with CDK8 and MED12
 MED1 recombinant protein  >5 publications with CDK8 and MED1
 MED15 recombinant protein  >4 publications with CDK8 and MED15
 MED17 recombinant protein  >4 publications with CDK8 and MED17
 MED24 recombinant protein  >3 publications with CDK8 and MED24
 MED14 recombinant protein  >3 publications with CDK8 and MED14
 MED6 recombinant protein  >3 publications with CDK8 and MED6
 MED23 recombinant protein  >2 publications with CDK8 and MED23
 MED21 recombinant protein  >2 publications with CDK8 and MED21
 Products by Pathway  Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
Diseases associated with CDK8 recombinant proteinOrgans/Tissues associated with CDK8 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >28 publications with CDK8 and Neoplasms
 Death Antibodies  >5 publications with CDK8 and Death
 Leukemia Antibodies  >5 publications with CDK8 and Leukemia
 Cell Transformation, Neoplastic Antibodies  >5 publications with CDK8 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >4 publications with CDK8 and Liver Diseases
 Neoplasm Metastasis Antibodies  >3 publications with CDK8 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >3 publications with CDK8 and Nervous System Diseases
 Necrosis Antibodies  >2 publications with CDK8 and Necrosis
 Breast Neoplasms Antibodies  >2 publications with CDK8 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with CDK8 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >4 publications with CDK8 and Muscle
 Heart Antibodies  >4 publications with CDK8 and Heart
 Eye Antibodies  >4 publications with CDK8 and Eye
 Liver Antibodies  >4 publications with CDK8 and Liver
 Bone Marrow Antibodies  >3 publications with CDK8 and Bone Marrow
 Brain Antibodies  >3 publications with CDK8 and Brain
 Prostate Antibodies  >2 publications with CDK8 and Prostate
 Testis Antibodies  >2 publications with CDK8 and Testis
 Lymph Antibodies  >2 publications with CDK8 and Lymph
 Intestine Antibodies  >2 publications with CDK8 and Intestine
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