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Tp53 recombinant protein :: Cellular tumor antigen p53 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS1265113
SDS-PAGE
Unit / Price
0.05 mg  /  $805 +1 FREE 8GB USB
0.2 mg  /  $1,075 +1 FREE 8GB USB
0.5 mg  /  $1,185 +1 FREE 8GB USB
1 mg  /  $1,685 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cellular tumor antigen p53 (Tp53), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Rat Cellular tumor antigen p53 protein

 Product Synonym Names    Tumor suppressor p53
 Product Gene Name   

Tp53 recombinant protein

[Similar Products]
 Product Synonym Gene Name    P53 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS1265113 COA    COA PDF
 Sequence Positions    1-391aa; Full Length
 Sequence    MEDSQSDMSI ELPLSQETFS CLWKLLPPDD ILPTTATGSP NSMEDLFLPQ DVAELLEGPE EALQVSAPAA QEPGTEAPAP VAPASATPWP LSSSVPSQKT YQGNYGFHLG FLQSGTAKSV MCTYSISLNK LFCQLAKTCP VQLWVTSTPP PGTRVRAMAI YKKSQHMTEV VRRCPHHERC SDGDGLAPPQ HLIRVEGNPY AEYLDDRQTF RHSVVVPYEP PEVGSDYTTI HYKYMCNSSC MGGMNRRPIL TIITLEDSSG NLLGRDSFEV RVCACPGRDR RTEEENFRKK EEHCPELPPG SAKRALPTST SSSPQQKKKP LDGEYFTLKI RGRERFEMFR ELNEALELKD ARAAEESGDS RAHSSYPKTK KGQSTSRHKK PMIKKVGPDS D
 3D Structure    ModBase 3D Structure for P10361
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE.
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Production Note    Special Offer: The E. coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E. coli host-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E. coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E. coli host-expressed protein for the fastest delivery among all hosts. Please contact us or email to support@mybiosource.com for more details.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Tp53 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Tp53 recombinant protein

   Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction ses to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and se to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
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 SDS-PAGE of Tp53 recombinant protein    Tp53 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for Tp53. It may not necessarily be applicable to this product.
 NCBI GI #    189083686
 NCBI GeneID    24842
 NCBI Accession #    NP_112251.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_030989.3 [Other Products]
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 UniProt Primary Accession #    P10361 [Other Products]
 UniProt Secondary Accession #    O09168; Q4KMA9; Q66HM0 [Other Products]
 UniProt Related Accession #    P10361 [Other Products]
 Molecular Weight    47.5kD
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 NCBI Official Full Name    cellular tumor antigen p53
 NCBI Official Synonym Full Names    tumor protein p53
 NCBI Official Symbol    Tp53 [Similar Products]
 NCBI Official Synonym Symbols   
p53; Trp53
[Similar Products]
 NCBI Protein Information    cellular tumor antigen p53
 UniProt Protein Name    Cellular tumor antigen p53
 UniProt Synonym Protein Names   
Tumor suppressor p53
 Protein Family    Cellular tumor antigen
 UniProt Gene Name    Tp53 [Similar Products]
 UniProt Synonym Gene Names    P53 [Similar Products]
 UniProt Entry Name    P53_RAT
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 NCBI Summary for Tp53    This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it is believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Alternatively spliced transcript variants have been found for this gene, but the biological validity of the variants has not been determined. p53 pseudogenes have been found on chromosomes 9 and 18. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Tp53    p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: DNA-binding; Tumor suppressor; Transcription factor; Motility/polarity/chemotaxis; Activator; Nuclear receptor co-regulator

Cellular Component: chromatin; cytoplasm; cytosol; endoplasmic reticulum; mitochondrial matrix; mitochondrion; nuclear body; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body; protein complex; replication fork; transcription factor complex; transcription factor TFIID complex

Molecular Function: ATP binding; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; DNA binding; double-stranded DNA binding; enzyme binding; histone acetyltransferase binding; histone deacetylase regulator activity; identical protein binding; metal ion binding; p53 binding; protease binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein kinase binding; protein N-terminus binding; protein phosphatase 2A binding; protein phosphatase binding; protein self-association; receptor tyrosine kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: aging; apoptosis; B cell lineage commitment; cell aging; cell cycle arrest; cellular response to glucose starvation; central nervous system development; cerebellum development; chromatin assembly; chromosome breakage; chromosome organization and biogenesis; circadian behavior; determination of adult life span; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; DNA strand renaturation; double-strand break repair; embryonic development ending in birth or egg hatching; embryonic organ development; entrainment of circadian clock by photoperiod; ER overload response; G1 DNA damage checkpoint; gastrulation; heart development; in utero embryonic development; mitochondrial DNA repair; multicellular organism growth; multicellular organismal development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of DNA replication; negative regulation of fibroblast proliferation; negative regulation of mitotic cell cycle; negative regulation of neuroblast proliferation; negative regulation of proteolysis; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; neuron apoptosis; nucleotide-excision repair; positive regulation of apoptosis; positive regulation of cell cycle; positive regulation of histone deacetylation; positive regulation of leukocyte migration; positive regulation of neuron apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein oligomerization; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; protein import into nucleus, translocation; protein localization; protein stabilization; protein tetramerization; Ras protein signal transduction; regulation of apoptosis; regulation of cell cycle; regulation of cell proliferation; regulation of intracellular pH; regulation of neuron apoptosis; regulation of tissue remodeling; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; release of cytochrome c from mitochondria; response to amino acid stimulus; response to antibiotic; response to caffeine; response to cytokine stimulus; response to DNA damage stimulus; response to drug; response to gamma radiation; response to hyperoxia; response to inorganic substance; response to metal ion; response to organic cyclic substance; response to organic nitrogen; response to oxidative stress; response to retinoic acid; response to salt stress; response to UV; response to UV-B; response to vitamin B3; response to X-ray; rRNA transcription; somitogenesis; T cell differentiation in the thymus; T cell lineage commitment; T cell proliferation during immune response; transcription from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; viral reproduction; wound healing
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Product References and Citations for Tp53 recombinant protein

   Nucleotide sequence of a cDNA encoding the rat p53 nuclear oncoprotein.Soussi T.Nucleic Acids Res. 16:11384-11384(1988) Structure of the rat p53 tumor suppressor gene.Hulla J.E., Schneider R.P.Nucleic Acids Res. 21:713-717(1993) Glucose catabolism in cancer cells. The type II hexokinase promoter contains functionally active response elements for the tumor suppressor p53.Mathupala S.P., Heese C., Pedersen P.L.J. Biol. Chem. 272:22776-22780(1997) Heat shock protein 84 forms a complex with mutant p53 protein predominantly within a cytoplasmic compartment of the cell.Sepehrnia B., Paz I.B., Dasgupta G., Momand J.J. Biol. Chem. 271:15084-15090(1996) p53 opens the mitochondrial permeability transition pore to trigger necrosis.Vaseva A.V., Marchenko N.D., Ji K., Tsirka S.E., Holzmann S., Moll U.M.Cell 149:1536-1548(2012)
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 Research Articles on Tp53    1. The interaction between GSK3beta and p53 is increased in kidneys of diabetic rats
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Tp53 recombinant proteinPathways associated with Tp53 recombinant protein
 Reference Product  PubMed Publications
 Mdm2 recombinant protein  >925 publications with Tp53 and Mdm2
 Cdkn1a recombinant protein  >700 publications with Tp53 and Cdkn1a
 Atm recombinant protein  >461 publications with Tp53 and Atm
 Bcl2 recombinant protein  >180 publications with Tp53 and Bcl2
 Chek2 recombinant protein  >151 publications with Tp53 and Chek2
 Mdm4 recombinant protein  >101 publications with Tp53 and Mdm4
 Crebbp recombinant protein  >24 publications with Tp53 and Crebbp
 Usp7 recombinant protein  >17 publications with Tp53 and Usp7
 Chek1 recombinant protein  >16 publications with Tp53 and Chek1
 Prkdc recombinant protein  >15 publications with Tp53 and Prkdc
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Diseases associated with Tp53 recombinant proteinOrgans/Tissues associated with Tp53 recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3317 publications with Tp53 and Carcinoma
 Breast Neoplasms Antibodies  >1258 publications with Tp53 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >821 publications with Tp53 and Colorectal Neoplasms
 Carcinoma, Squamous Cell Antibodies  >779 publications with Tp53 and Carcinoma, Squamous Cell
 Glioma Antibodies  >654 publications with Tp53 and Glioma
 Central Nervous System Neoplasms Antibodies  >568 publications with Tp53 and Central Nervous System Neoplasms
 Neoplasm Metastasis Antibodies  >477 publications with Tp53 and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >456 publications with Tp53 and Cell Transformation, Neoplastic
 Sarcoma Antibodies  >419 publications with Tp53 and Sarcoma
 Liver Neoplasms Antibodies  >415 publications with Tp53 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >954 publications with Tp53 and Lung
 Brain Antibodies  >707 publications with Tp53 and Brain
 Colon Antibodies  >473 publications with Tp53 and Colon
 Prostate Antibodies  >269 publications with Tp53 and Prostate
 Kidney Antibodies  >184 publications with Tp53 and Kidney
 Ovary Antibodies  >101 publications with Tp53 and Ovary
 Pancreas Antibodies  >101 publications with Tp53 and Pancreas
 Heart Antibodies  >74 publications with Tp53 and Heart
 Embryonic Tissue Antibodies  >62 publications with Tp53 and Embryonic Tissue
 Adrenal Gland Antibodies  >24 publications with Tp53 and Adrenal Gland
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