Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Recombinant Protein rightarrow F8  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • MyD88 (CT) Antibody
 • Vimentin Antibody
 • hepatic lipase, HL ELISA Kit
 • Vaccinia Virus, A27L Antibody
 • 4E-BP1 Antibody
 • J domain-containing protein spf31 (spf31) Recombinant Protein
 • Basic Fibroblast Growth Factor (bFGF - FGF2) ELISA Kit
 • 60S ribosomal protein L32 (RpL32) Recombinant Protein
 • ITGA2B Antibody
 • alpha2 Macroglobulin Precursor (PRE-alpha2 MG) ELISA Kit
 • Tenascin X (TNX) ELISA Kit
 • C11orf68 Antibody
 • Auxin-responsive protein IAA26 (IAA26) Recombinant Protein
 • Protein L-Isoaspartate-O-Methyltran sferase (PCMT1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

F8 recombinant protein :: Coagulation Factor VIII Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS142874 F8 recombinant protein
Unit / Price
250 IU  /  $1,375 +1 FREE 8GB USB
500 IU  /  $2,475 +3 FREE 8GB USB
1000 IU  /  $4,170 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor VIII (F8), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Coagulation Factor VIII

 Product Synonym Names    F8 Human; Coagulation Factor-VIII Human Recombinant; Coagulation factor VIII; Procoagulant component; Antihemophilic factor; AHF; F8; F8C; F8B; HEMA; FVIII; DXS1253E; F8 protein
 Product Gene Name   

F8 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    134500
 3D Structure    ModBase 3D Structure for P00451
Section Bar
 Host    Chinese Hamster Ovarian Cells (CHO)
Section Bar
 Purity/Purification    Greater than 97.0% as determined by SDS-PAGE.
 Form/Format    The protein was lyophilized from a solution containing 50mM histidine, 0.3M NaCl, 5mM CaCl2, 0.02% Tween 80, 20mM sucrose, pH- 6.8.
Sterile Filtered White lyophilized (freeze-dried) powder.
Section Bar
 Solubility    It is recommended to reconstitute 250IU lyophilized Factor-VIII in 5ml sterile 18M-cm H2O, which can then be further diluted to other aqueous solutions.
 Biological Activity    The specific activity was found to be 7058IU/mg.
Section Bar
 Preparation and Storage    Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Factor-VIII should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of F8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for F8 recombinant protein

   Description: Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having a total amino acids of 1438 (170kd) and consisting of two dimer chains 80 kD and 90 kD.The Factor-VIII lacks central region of B-domain region and is purified by proprietary chromatographic techniques.

Introduction: Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
 Product Categories/Family for F8 recombinant protein    RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Coagulation Factors
NCBI/Uniprot data below describe general gene information for F8. It may not necessarily be applicable to this product.
 NCBI GI #    4503647
 NCBI GeneID    2157
 NCBI Accession #    NP_000123.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000132.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P00451 [Other Products]
 UniProt Secondary Accession #    Q14286; Q5HY69 [Other Products]
 UniProt Related Accession #    P00451 [Other Products]
 Molecular Weight    24,641 Da
Table BarTOPTable Bar
 NCBI Official Full Name    coagulation factor VIII isoform a
 NCBI Official Synonym Full Names    coagulation factor VIII, procoagulant component
 NCBI Official Symbol    F8 [Similar Products]
 NCBI Official Synonym Symbols   
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
[Similar Products]
 NCBI Protein Information    coagulation factor VIII; antihemophilic factor; coagulation factor VIIIc; factor VIII F8B
 UniProt Protein Name    Coagulation factor VIII
 UniProt Synonym Protein Names   
Antihemophilic factor; AHF; Procoagulant componentCleaved into the following 4 chains:Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain
 Protein Family    Coagulation factor
 UniProt Gene Name    F8 [Similar Products]
 UniProt Synonym Gene Names    F8C; AHF [Similar Products]
 UniProt Entry Name    FA8_HUMAN
Section Bar
 NCBI Summary for F8    This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for F8    F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity; oxidoreductase activity

Biological Process: platelet activation; platelet degranulation; acute-phase response; blood coagulation; proteolysis; blood coagulation, intrinsic pathway

Disease: Hemophilia A; Factor Viii Deficiency
Table BarTOPTable Bar
 Research Articles on F8    1. All four domains of FIXa wrap across FVIIIa that spans the co-factor binding surface of A2, A3 and C1 domains.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with F8 recombinant proteinPathways associated with F8 recombinant protein
 Reference Product  PubMed Publications
 VWF recombinant protein  >61 publications with F8 and VWF
 F10 recombinant protein  >26 publications with F8 and F10
 SERPINE1 recombinant protein  >3 publications with F8 and SERPINE1
 LMAN1 recombinant protein  >3 publications with F8 and LMAN1
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
Diseases associated with F8 recombinant proteinOrgans/Tissues associated with F8 recombinant protein
 Disease Name  Pubmed Publications
 Hemophilia A Antibodies  >535 publications with F8 and Hemophilia A
 Hemorrhage Antibodies  >137 publications with F8 and Hemorrhage
 Nervous System Diseases Antibodies  >108 publications with F8 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >92 publications with F8 and Cardiovascular Diseases
 Thrombosis Antibodies  >75 publications with F8 and Thrombosis
 Brain Diseases Antibodies  >52 publications with F8 and Brain Diseases
 Heart Diseases Antibodies  >30 publications with F8 and Heart Diseases
 Kidney Diseases Antibodies  >27 publications with F8 and Kidney Diseases
 Inflammation Antibodies  >26 publications with F8 and Inflammation
 Venous Thrombosis Antibodies  >26 publications with F8 and Venous Thrombosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >517 publications with F8 and Blood
 Brain Antibodies  >149 publications with F8 and Brain
 Vascular Antibodies  >97 publications with F8 and Vascular
 Muscle Antibodies  >55 publications with F8 and Muscle
 Kidney Antibodies  >54 publications with F8 and Kidney
 Heart Antibodies  >40 publications with F8 and Heart
 Liver Antibodies  >38 publications with F8 and Liver
 Nerve Antibodies  >27 publications with F8 and Nerve
 Lung Antibodies  >26 publications with F8 and Lung
 Skin Antibodies  >24 publications with F8 and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions