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CYP24A1 recombinant protein :: Cytochrome P450 24A1 (CYP24A1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2018602 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $150 +1 FREE 8GB USB
0.05 mg  /  $295 +1 FREE 8GB USB
0.1 mg  /  $460 +1 FREE 8GB USB
0.2 mg  /  $570 +1 FREE 8GB USB
0.5 mg  /  $1,105 +1 FREE 8GB USB
1 mg  /  $1,645 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytochrome P450 24A1 (CYP24A1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Cytochrome P450 24A1 (CYP24A1)

 Product Gene Name   

CYP24A1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKR MCIGRRLAEL QLHLALCWII QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR
 Chromosome Location    Chromosome: 2; NC_000068.7 (170482965..170497138, complement). Location: 2 H3; 2 91.91 cM
 3D Structure    ModBase 3D Structure for Q64441
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    His260~Arg514 (Accession # Q64441) with two N-terminal Tags, His-tag and T7-tag
 Subcellular Location    Mitochondrion
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    8.9
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of CYP24A1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CYP24A1 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for CYP24A1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of CYP24A1 recombinant protein    CYP24A1 recombinant protein SDS-PAGE image
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 SDS-PAGE of CYP24A1 recombinant protein    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
CYP24A1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for CYP24A1. It may not necessarily be applicable to this product.
 NCBI GI #    6753572
 NCBI GeneID    13081
 NCBI Accession #    NP_034126.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_009996.3 [Other Products]
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 UniProt Primary Accession #    Q64441 [Other Products]
 UniProt Related Accession #    Q64441 [Other Products]
 Molecular Weight    33.2kDa
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 NCBI Official Full Name    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
 NCBI Official Synonym Full Names    cytochrome P450, family 24, subfamily a, polypeptide 1
 NCBI Official Symbol    Cyp24a1 [Similar Products]
 NCBI Official Synonym Symbols   
CP24; Cyp24; 24-OHase
[Similar Products]
 NCBI Protein Information    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450, 24; cytochrome P450 24A1; cytochrome P450-CC24; cytochrome P450, 24a1; vitamin D(3) 24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase
 UniProt Protein Name    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
 UniProt Synonym Protein Names   
Cytochrome P450 24A1; Cytochrome P450-CC24
 Protein Family    1,25-dihydroxyvitamin D(3) 24-hydroxylase
 UniProt Gene Name    Cyp24a1 [Similar Products]
 UniProt Synonym Gene Names    Cyp-24; Cyp24; 24-OHase [Similar Products]
 UniProt Entry Name    CP24A_MOUSE
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 NCBI Summary for CYP24A1    The protein encoded by this gene localizes to the mitochondrion, where it degrades calcitriol to calcitetrol. This gene is upregulated by binding of calcitriol to the upstream regulatory region and to a downstream enhancer region, thereby allowing calcitriol to autoregulate its concentration in the cell. The encoded protein may also play a role in calcium homeostasis. [provided by RefSeq, Aug 2015]
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 UniProt Comments for CYP24A1    CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.126; Mitochondrial

Cellular Component: mitochondrion

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity; monooxygenase activity

Biological Process: response to vitamin D; vitamin D metabolic process
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 Research Articles on CYP24A1    1. Data suggest that control of CYP24A1 increase during diabetes has a beneficial effect on senescence induction and caspase-3 increased expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CYP24A1 recombinant proteinPathways associated with CYP24A1 recombinant protein
 Reference Product  PubMed Publications
 Vdr recombinant protein  >146 publications with CYP24A1 and Vdr
 Fgf23 recombinant protein  >18 publications with CYP24A1 and Fgf23
 Pth recombinant protein  >17 publications with CYP24A1 and Pth
 Trpv6 recombinant protein  >13 publications with CYP24A1 and Trpv6
 Ahr recombinant protein  >1 publications with CYP24A1 and Ahr
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cytochrome P450 Pathway antibodies  Cytochrome P450 Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Metapathway Biotransformation antibodies  Metapathway Biotransformation Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
Diseases associated with CYP24A1 recombinant proteinOrgans/Tissues associated with CYP24A1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >83 publications with CYP24A1 and Neoplasms
 Hypercalcemia Antibodies  >21 publications with CYP24A1 and Hypercalcemia
 Breast Neoplasms Antibodies  >20 publications with CYP24A1 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >20 publications with CYP24A1 and Prostatic Neoplasms
 Rickets Antibodies  >16 publications with CYP24A1 and Rickets
 Kidney Diseases Antibodies  >14 publications with CYP24A1 and Kidney Diseases
 Inflammation Antibodies  >10 publications with CYP24A1 and Inflammation
 Hypercalciuria Antibodies  >8 publications with CYP24A1 and Hypercalciuria
 Lung Neoplasms Antibodies  >8 publications with CYP24A1 and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >7 publications with CYP24A1 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >51 publications with CYP24A1 and Kidney
 Bone Antibodies  >51 publications with CYP24A1 and Bone
 Embryonic Tissue Antibodies  >3 publications with CYP24A1 and Embryonic Tissue
 Mammary Gland Antibodies  >2 publications with CYP24A1 and Mammary Gland
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