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Clec7a recombinant protein :: Dectin-1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS637803 Clec7a recombinant protein
Unit / Price
0.05 mg  /  $710 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dectin-1 (Clec7a), Recombinant Protein

 Also Known As   

Dectin-1, Recombinant, Human (C-type lectin domain family 7 member A, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Beta-glucan receptor, C-type lectin superfamily member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)

 Product Gene Name   

Clec7a recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 6; NC_000072.6 (129461591..129472777, complement). Location: 6 F3; 6
 3D Structure    ModBase 3D Structure for Q6QLQ4
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 Purity/Purification    Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
 Form/Format    Supplied as a lyophilized powder in PBS.
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 Activity    Measured by its ability to bind biotinylated laminarin (1, 3- -glucan) with an estimated KD < 5nM.
 Endotoxin Level    < 0.1 EU per 1 g of the protein as determined by the LAL method.
 Reconstitution    It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
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 Preparation and Storage    Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of Clec7a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Clec7a recombinant protein

   Dectin-1, also known as CLEC7A and the -glucan receptor, is a 33kD type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9,11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% aa sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.
 Product Categories/Family for Clec7a recombinant protein    Molecular Biology; MB-Receptors
NCBI/Uniprot data below describe general gene information for Clec7a. It may not necessarily be applicable to this product.
 NCBI GI #    8102031
 NCBI GeneID    56644
 NCBI Accession #    AAF72710.1 [Other Products]
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 UniProt Primary Accession #    Q6QLQ4 [Other Products]
 UniProt Secondary Accession #    Q8K1L4; Q9JI50; E9QPW2 [Other Products]
 UniProt Related Accession #    Q6QLQ4 [Other Products]
 Molecular Weight    The recombinant human Dectin-1, generated by the removal of the signal peptide region, is a monomeric protein. Based on N-terminal sequencing, the protein begins with the 10X histidine tag and has a calculated molecular mass of approximately 16.8 kD. [Similar Products]
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 NCBI Official Full Name    dendritic cell-associated C-type lectin-1
 NCBI Official Synonym Full Names    C-type lectin domain family 7, member a
 NCBI Official Symbol    Clec7a [Similar Products]
 NCBI Official Synonym Symbols   
BGR; beta-GR; Clecsf12
[Similar Products]
 NCBI Protein Information    C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12
 UniProt Protein Name    C-type lectin domain family 7 member A
 UniProt Synonym Protein Names   
Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1
 Protein Family    C-type lectin domain family
 UniProt Gene Name    Clec7a [Similar Products]
 UniProt Synonym Gene Names    Bgr; Clecsf12; Dectin1; DC-associated C-type lectin 1; Dectin-1 [Similar Products]
 UniProt Entry Name    CLC7A_MOUSE
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 UniProt Comments for Clec7a    dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: zymosan receptor activity; protein binding; opsonin binding; zymosan binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; polysaccharide binding

Biological Process: immune system process; cell surface pattern recognition receptor signaling pathway; phagocytosis, recognition; cell recognition; response to molecule of fungal origin; pattern recognition receptor signaling pathway; positive regulation of tumor necrosis factor production; cell-cell adhesion; leukocyte activation during immune response; positive regulation of phagocytosis; innate immune response; response to yeast; inflammatory response; phagocytosis, engulfment; detection of yeast
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 Research Articles on Clec7a    1. These data suggest that hypoxia-induced fungal cell wall changes influence the activation of innate effector cells at sites of hyphal tissue invasion via the mammalian beta-glucan receptor dectin-1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Clec7a recombinant proteinDiseases associated with Clec7a recombinant protein
 Products by Pathway  Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with Clec7a and Inflammation
 Necrosis Antibodies  >4 publications with Clec7a and Necrosis
 Colitis Antibodies  >2 publications with Clec7a and Colitis
 Lung Diseases Antibodies  >2 publications with Clec7a and Lung Diseases
 Non-alcoholic Fatty Liver Disease Antibodies  >1 publications with Clec7a and Non-alcoholic Fatty Liver Disease
 Fatty Liver Antibodies  >1 publications with Clec7a and Fatty Liver
 Neoplasms Antibodies  >1 publications with Clec7a and Neoplasms
 Liver Diseases Antibodies  >1 publications with Clec7a and Liver Diseases
Organs/Tissues associated with Clec7a recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with Clec7a and Lung
 Skin Antibodies  >3 publications with Clec7a and Skin
 Bone Marrow Antibodies  >2 publications with Clec7a and Bone Marrow
 Uterus Antibodies  >1 publications with Clec7a and Uterus
 Spleen Antibodies  >1 publications with Clec7a and Spleen
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