Product References and Citations for
DHFR recombinant protein
The functional human dihydrofolate reductase gene.Chen M.-J., Shimada T., Moulton A.D., Cline A., Humphries R.K., Maizel J., Nienhuis A.W.J. Biol. Chem. 259:3933-3943(1984)
The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase.Masters J.N., Attardi G.Gene 21:59-63(1983)
Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes.Yang J.K., Masters J.N., Attardi G.J. Mol. Biol. 176:169-187(1984)
Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004)
The DNA sequence and comparative analysis of human chromosome 5.Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S., Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.Nature 431:268-274(2004)
Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.Banka S., Blom H.J., Walter J., Aziz M., Urquhart J., Clouthier C.M., Rice G.I., de Brouwer A.P., Hilton E., Vassallo G., Will A., Smith D.E., Smulders Y.M., Wevers R.A., Steinfeld R., Heales S., Crow Y.J., Pelletier J.N., Jones S., Newman W.G.Am. J. Hum. Genet. 88:216-225(2011)
Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011)
The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1)
is expressed and functional.McEntee G., Minguzzi S., O'Brien K., Ben Larbi N., Loscher C., O'Fagain C., Parle-McDermott A.Proc. Natl. Acad. Sci. U.S.A. 108:15157-15162(2011)
Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria.Anderson D.D., Quintero C.M., Stover P.J.Proc. Natl. Acad. Sci. U.S.A. 108:15163-15168(2011)
Crystal structure of human dihydrofolate reductase complexed with folate.Oefner C., D'Arcy A., Winkler F.K.Eur. J. Biochem. 174:377-385(1988)
Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate.Davies J.F., Delcamp T.J., Prendergast N.J., Ashford V.A., Freisheim J.H., Kraut J.Biochemistry 29:9467-9479(1990)
Sequence-specific 1H and 15N resonance assignments for human dihydrofolate reductase in solution.Stockman B.J., Nirmala N.R., Wagner G., Delcamp T.J., Deyarman M.T., Freisheim J.H.Biochemistry 31:218-229(1992)
Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.Lewis W.S., Cody V., Galitsky N., Luft J.R., Pangborn W., Chunduru S.K., Spencer H.T., Appleman J.R., Blakley R.L.J. Biol. Chem. 270:5057-5064(1995)
Comparison of two independent crystal structures of human dihydrofolate reductase ternary complexes reduced with nicotinamide adenine dinucleotide phosphate and the very tight-binding inhibitor PT523.Cody V., Galitsky N., Luft J.R., Pangborn W., Blakley R.L., Gangjee A.Biochemistry 36:13897-13903(1997)
Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents.Gangjee A., Vidwans A.P., Vasudevan A., Queener S.F., Kisliuk R.L., Cody V., Li R., Galitsky N., Luft J.R., Pangborn W.J. Med. Chem. 41:3426-3434(1998)
Atomic structures of human dihydrofolate reductase complexed with NADPH and two lipophilic antifolates at 1.09 A and 1.05 A resolution.Klon A.E., Heroux A., Ross L.J., Pathak V., Johnson C.A., Piper J.R., Borhani D.W.J. Mol. Biol. 320:677-693(2002)
Analysis of two polymorphic forms of a pyridopyrimidine N9-C10 reversed-bridge antifolate binary complex with human dihydrofolate reductase.Cody V., Galitsky N., Luft J.R., Pangborn W., Gangjee A.Acta Crystallogr. D 59:654-661(2003)
Analysis of three crystal structure determinations of a 5-methyl-6-N-methylanilino pyridopyrimidine antifolate complex with human dihydrofolate reductase.Cody V., Luft J.R., Pangborn W., Gangjee A.Acta Crystallogr. D 59:1603-1609(2003)
Structure determination of tetrahydroquinazoline antifolates in complex with human and Pneumocystis carinii dihydrofolate reductase
correlations between enzyme selectivity and stereochemistry.Cody V., Luft J.R., Pangborn W., Gangjee A., Queener S.F.Acta Crystallogr. D 60:646-655(2004)
Understanding the role of Leu22 variants in methotrexate resistance
comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH.Cody V., Luft J.R., Pangborn W.Acta Crystallogr. D 61:147-155(2005)
Solution structure of human dihydrofolate reductase in its complex with trimethoprim and NADPH.Kovalevskaya N.V., Smurnyy Y.D., Polshakov V.I., Birdsall B., Bradbury A.F., Frenkiel T., Feeney J.J. Biomol. NMR 33:69-72(2005)
Novel boron-containing, nonclassical antifolates
synthesis and preliminary biological and structural evaluation.Reynolds R.C., Campbell S.R., Fairchild R.G., Kisliuk R.L., Micca P.L., Queener S.F., Riordan J.M., Sedwick W.D., Waud W.R., Leung A.K., Dixon R.W., Suling W.J., Borhani D.W.J. Med. Chem. 50:3283-3289(2007)
Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes.Cody V., Pace J., Makin J., Piraino J., Queener S.F., Rosowsky A.Biochemistry 48:1702-1711(2009)
Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance.Volpato J.P., Yachnin B.J., Blanchet J., Guerrero V., Poulin L., Fossati E., Berghuis A.M., Pelletier J.N.J. Biol. Chem. 284:20079-20089(2009)
Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthienopyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents.Gangjee A., Li W., Kisliuk R.L., Cody V., Pace J., Piraino J., Makin J.J. Med. Chem. 52:4892-4902(2009)
Dihydrofolate reductase deficiency due to a homozygous DHFR mutation causes megaloblastic anemia and cerebral folate deficiency leading to severe neurologic disease.Cario H., Smith D.E., Blom H., Blau N., Bode H., Holzmann K., Pannicke U., Hopfner K.P., Rump E.M., Ayric Z., Kohne E., Debatin K.M., Smulders Y., Schwarz K.Am. J. Hum. Genet. 88:226-231(2011)
+Additional computationally mapped references.<p>Provides general information on the entry.