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DsbA recombinant protein :: Disulfide oxidoreductase (DsbA) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS203035
SDS-Page
Unit / Price
0.1 mg  /  $255 +1 FREE 8GB USB
0.5 mg  /  $535 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Disulfide oxidoreductase (DsbA), Recombinant Protein

 Also Known As   

Disulfide oxidoreductase (DsbA), 20-208 aa, E Coli, E Coli

 Product Synonym Names    Disulfide oxidoreductase A; dsbA; rpbB; Disulfide oxidoreductase (DsbA) E Coli; DsDNA-binding protein A; Double-stranded DNA-binding protein; Disulfide oxidoreductase A periplasmic protein disulfide isomerase I; Thiol disulfide interchange protein dsbA.
 Product Gene Name   

DsbA recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK
 3D Structure    ModBase 3D Structure for P0AEG4
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    Liquid. 20mM Tris pH 7.5, 2mM EDTA
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of DsbA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for DsbA recombinant protein

   Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide (1-20 amino acids). Recombinant DsbC was expressed in E. coli and purified by using conventional chromatography techniques.
 Product Categories/Family for DsbA recombinant protein    Heat Shock Proteins
 Applications Tested/Suitable for DsbA recombinant protein   

SDS-PAGE

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 SDS-Page of DsbA recombinant protein    DsbA recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for DsbA. It may not necessarily be applicable to this product.
 NCBI GI #    16131701
 NCBI GeneID    948353
 NCBI Accession #    NP_418297 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P0AEG4 [Other Products]
 UniProt Secondary Accession #    P24991; Q2M8F8; Q46951; Q46952 [Other Products]
 Molecular Weight    21.2kDa (190aa), confirmed by MALDI-TOF.
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 NCBI Official Full Name    periplasmic protein disulfide isomerase I
 NCBI Official Symbol    dsbA [Similar Products]
 NCBI Official Synonym Symbols   
dsf; ECK3852; iarA; JW3832; ppfA
[Similar Products]
 NCBI Protein Information    periplasmic protein disulfide isomerase I
 UniProt Protein Name    Thiol:disulfide interchange protein DsbA
 Protein Family    Double-stranded DNA-binding protein
 UniProt Gene Name    dsbA [Similar Products]
 UniProt Synonym Gene Names    dsf; ppfA [Similar Products]
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 NCBI Summary for DsbA    DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
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 UniProt Comments for DsbA    Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
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Product References and Citations for DsbA recombinant protein

   Charbonnier, J. B. et al. (1999). Protein Sci. 8:96-105; Inaba, K. et al. (2004). J. Biol. Chem. 279:6761-6768.
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 Research Articles on DsbA    1. analysis of mutants that map to two areas in the structure of DsbB, one located between the two first transmembrane segments where the quinone ring binds and the other located in the second periplasmic loop of DsbB, which interacts with DsbA
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DsbA recombinant proteinPathways associated with DsbA recombinant protein
 Reference Product  PubMed Publications
 dsbB recombinant protein  >147 publications with DsbA and dsbB
 phoA recombinant protein  >19 publications with DsbA and phoA
 dsbE recombinant protein  >7 publications with DsbA and dsbE
 trxB recombinant protein  >2 publications with DsbA and trxB
 Products by Pathway  Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway Diagram
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