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Dnak recombinant protein

Scan QR to view Datasheet Catalog #    MBS203029
SDS-Page
Unit / Price
0.1 mg  /  $255 +1 FREE 8GB USB
0.5 mg  /  $535 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dnak, Recombinant Protein

 Also Known As   

Dnak, 385-638aa, E Coli, E Coli

 Product Synonym Names    Dnak (C-term; 385-638); Substrate binding domain; Heat shock protein 70; Heat shock 70 kDa protein; HSP70; Chaperone protein dnaK; Chaperone Hsp 70; Co chaperone with DnaJ; dnaK; Heat shock 70 kDa protein
 Product Gene Name   

Dnak recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKT AIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK
 3D Structure    ModBase 3D Structure for P0A6Y8
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5mM DTT, 10% glycerol
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    E Coli
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of Dnak recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Dnak recombinant protein

   DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DNAK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus
 Product Categories/Family for Dnak recombinant protein    Heat Shock Proteins
 Applications Tested/Suitable for Dnak recombinant protein   

SDS-PAGE

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 SDS-Page of Dnak recombinant protein    Dnak recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for Dnak. It may not necessarily be applicable to this product.
 NCBI GI #    16128008
 NCBI GeneID    944750
 NCBI Accession #    NP_414555 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P0A6Y8 [Other Products]
 UniProt Secondary Accession #    P04475 [Other Products]
 Molecular Weight    27.7kDa (255aa), confirmed by MALDI-TOF.
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 NCBI Official Full Name    chaperone Hsp70, with co-chaperone DnaJ
 NCBI Official Symbol    dnaK [Similar Products]
 NCBI Official Synonym Symbols   
ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
[Similar Products]
 NCBI Protein Information    chaperone Hsp70, with co-chaperone DnaJ
 UniProt Protein Name    Chaperone protein DnaK
 UniProt Synonym Protein Names   
HSP70; Heat shock 70 kDa protein; Heat shock protein 70
 Protein Family    Chaperone protein
 UniProt Gene Name    dnaK [Similar Products]
 UniProt Synonym Gene Names    groP; grpF; seg [Similar Products]
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 NCBI Summary for Dnak    ATP-regulated binding and release of polypeptide substrates. [More information is available at EcoGene: EG10241]. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity . [More information is available at EcoCyc: EG10241].
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 UniProt Comments for Dnak    Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
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Product References and Citations for Dnak recombinant protein

   Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; Zhu et al., (1996) Science 272, 1606-1614.; Naoki tanaka., et al (2002) PNAS 26 (99)15398-15403;
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 Research Articles on Dnak    1. the mutant K-12 DeltadnaK was more sensitive to Ultrasound with c. 2.5 log (CFU per ml) reduction in comparison to their isogenic wild-type E. coli K-12. This indicates that the dnaK gene participates in general stress response and more specifically to hyperosmotic stress.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Dnak recombinant proteinPathways associated with Dnak recombinant protein
 Reference Product  PubMed Publications
 dnaJ recombinant protein  >715 publications with Dnak and dnaJ
 grpE recombinant protein  >509 publications with Dnak and grpE
 clpB recombinant protein  >201 publications with Dnak and clpB
 htpG recombinant protein  >48 publications with Dnak and htpG
 cbpA recombinant protein  >24 publications with Dnak and cbpA
 hscB recombinant protein  >9 publications with Dnak and hscB
 pnp recombinant protein  >6 publications with Dnak and pnp
 groL recombinant protein  >3 publications with Dnak and groL
 groS recombinant protein  >1 publications with Dnak and groS
 Products by Pathway  Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
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