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DAD1 recombinant protein :: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS717181
SDS-Page
Unit / Price
0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Recombinant Protein

 Also Known As   

Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

 Product Synonym Names    Defender against cell death 1; DAD-1
 Product Gene Name   

DAD1 recombinant protein

[Similar Products]
 Matching Pairs    Antibody: DAD1 (MBS7041818)
Standard: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (MBS717181)
Comments: Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    2-113
 Sequence    SASVVSVISR FLEEYLSSTP QRLKLLDAYL LYILLTGALQ FGYCLLVGTF PFNSFLSGFI SCVGSFILAV CLRIQINPQN KADFQGISPE RAFADFLFAS TILHLVVMNF VG
 OMIM    600243
 3D Structure    ModBase 3D Structure for P61803
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 Host    E Coli
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 Purity/Purification    Greater than 90% as determined by SDS-PAGE. (lot specific)
 Form/Format    Liquid containing glycerol
 Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
 Sterility    Sterile filter available upon request.
 Endotoxin    Low endotoxin available upon request.
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 Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of DAD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for DAD1 recombinant protein

   Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
 Product Categories/Family for DAD1 recombinant protein    Apoptosis
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 SDS-Page of DAD1 recombinant protein    DAD1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for DAD1. It may not necessarily be applicable to this product.
 NCBI GI #    4503253
 NCBI GeneID    1603
 NCBI Accession #    NP_001335.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001344.3 [Other Products]
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 UniProt Primary Accession #    P61803 [Other Products]
 UniProt Secondary Accession #    O08552; O70364; P46966; P46968; Q6FGA3; Q96GB7; D3DS25 [Other Products]
 UniProt Related Accession #    P61803 [Other Products]
 Molecular Weight    39.8kD
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 NCBI Official Full Name    dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
 NCBI Official Synonym Full Names    defender against cell death 1
 NCBI Official Symbol    DAD1 [Similar Products]
 NCBI Official Synonym Symbols   
OST2
[Similar Products]
 NCBI Protein Information    dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
 UniProt Protein Name    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
 UniProt Synonym Protein Names   
Defender against cell death 1; DAD-1
 Protein Family    DASH complex
 UniProt Gene Name    DAD1 [Similar Products]
 UniProt Synonym Gene Names    Oligosaccharyl transferase subunit DAD1; DAD-1 [Similar Products]
 UniProt Entry Name    DAD1_HUMAN
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 NCBI Summary for DAD1    DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DAD1    DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: EC 2.4.99.18; Membrane protein, multi-pass; Glycan Metabolism - N-glycan biosynthesis; Endoplasmic reticulum; Apoptosis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane; oligosaccharyl transferase complex

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: apoptosis; blastocyst development; cellular protein metabolic process; negative regulation of apoptosis; post-translational protein modification; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; response to drug; response to nutrient
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Product References and Citations for DAD1 recombinant protein

   Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.Mol. Cell. Biol. 13:6367-6374(1993)
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 Research Articles on DAD1    1. single-nucleotide polymorphisms in DAD1 gene is associated with neuroendocrine tumor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DAD1 recombinant proteinPathways associated with DAD1 recombinant protein
 Reference Product  PubMed Publications
 STT3B recombinant protein  >2 publications with DAD1 and STT3B
 STT3A recombinant protein  >2 publications with DAD1 and STT3A
 MCL1 recombinant protein  >1 publications with DAD1 and MCL1
 PCNA recombinant protein  >1 publications with DAD1 and PCNA
 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-glycosylation By Oligosaccharyltransferase Pathway antibodies  N-glycosylation By Oligosaccharyltransferase Pathway Diagram
 N-glycosylation By Oligosaccharyltransferase Pathway antibodies  N-glycosylation By Oligosaccharyltransferase Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
Diseases associated with DAD1 recombinant proteinOrgans/Tissues associated with DAD1 recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >9 publications with DAD1 and Carcinoma
 Adenocarcinoma Antibodies  >5 publications with DAD1 and Adenocarcinoma
 Fetal Death Antibodies  >3 publications with DAD1 and Fetal Death
 Liver Diseases Antibodies  >3 publications with DAD1 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with DAD1 and Liver Neoplasms
 Inflammation Antibodies  >3 publications with DAD1 and Inflammation
 Immune System Diseases Antibodies  >2 publications with DAD1 and Immune System Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with DAD1 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >1 publications with DAD1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with DAD1 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with DAD1 and Blood
 Prostate Antibodies  >5 publications with DAD1 and Prostate
 Brain Antibodies  >5 publications with DAD1 and Brain
 Muscle Antibodies  >4 publications with DAD1 and Muscle
 Kidney Antibodies  >4 publications with DAD1 and Kidney
 Liver Antibodies  >3 publications with DAD1 and Liver
 Vascular Antibodies  >3 publications with DAD1 and Vascular
 Pancreas Antibodies  >3 publications with DAD1 and Pancreas
 Embryonic Tissue Antibodies  >2 publications with DAD1 and Embryonic Tissue
 Lung Antibodies  >2 publications with DAD1 and Lung
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