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DuSP3 recombinant protein

Scan QR to view Datasheet Catalog #    MBS204275
SDS-PAGE
Unit / Price
0.1 mg  /  $310 +1 FREE 8GB USB
0.5 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DuSP3, Recombinant Protein

★Popular Item★
 Also Known As   

DuSP3, 1-185aa, Human, His tag, E Coli

 Product Synonym Names    Dual specificity protein phosphatase 3; VHR
 Product Gene Name   

DuSP3 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
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 Purity/Purification    > 95 % by SDS - PAGE
 Form/Format    Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
 Concentration    1 mg/ml (determined by Bradford) (lot specific)
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 Antigen Species    Human
 Tag    His-tag
 Expression System    E Coli
 Bioactivity    Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH7.5 using 10mM of substrate.
 Assay    1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
2. Pre-warm reaction mix in heat block at 37C.
3. Add recombinant DuSP3 protein solution with various concentrations (1ug, 2ug, 3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37C for 10minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH and record the absorbance at 405nm.
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of DuSP3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for DuSP3 recombinant protein

   DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DuSP3 is expressed in both breast and ovarian tissues. DuSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DuSP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
 Product Categories/Family for DuSP3 recombinant protein    Cancer
 Applications Tested/Suitable for DuSP3 recombinant protein   

SDS-PAGE

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 SDS-PAGE of DuSP3 recombinant protein    DuSP3 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for DuSP3. It may not necessarily be applicable to this product.
 NCBI GI #    4758208
 NCBI GeneID    1845
 NCBI Accession #    NP_004081 [Other Products]
 NCBI GenBank Nucleotide #    NM_004090.3 [Other Products]
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 UniProt Secondary Accession #    Q5U0J1; Q8IYJ9; D3DX45 [Other Products]
 UniProt Related Accession #    P51452 [Other Products]
 Molecular Weight    22.6 kDa (205aa), confirmed by MALDI-TOF
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 NCBI Official Full Name    dual specificity protein phosphatase 3
 NCBI Official Synonym Full Names    dual specificity phosphatase 3
 NCBI Official Symbol    DUSP3 [Similar Products]
 NCBI Official Synonym Symbols   
VHR
[Similar Products]
 NCBI Protein Information    dual specificity protein phosphatase 3; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related
 UniProt Protein Name    Dual specificity protein phosphatase 3
 UniProt Synonym Protein Names   
Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR
 Protein Family    Dual specificity protein phosphatase
 UniProt Gene Name    DUSP3 [Similar Products]
 UniProt Synonym Gene Names    VHR; VHR [Similar Products]
 UniProt Entry Name    DUS3_HUMAN
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 NCBI Summary for DuSP3    The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DuSP3    DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: negative regulation of JNK cascade; nerve growth factor receptor signaling pathway; negative regulation of MAPKKK cascade; in utero embryonic development; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; negative regulation of T cell receptor signaling pathway; toll-like receptor 3 signaling pathway; positive regulation of mitotic cell cycle; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; negative regulation of T cell activation; inactivation of MAPK activity
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Product References and Citations for DuSP3 recombinant protein

   Todd JL., et al. (1999) J Biol Chem. 274(19):13271-80.
Keyse SM., et al. (1995) Biochim Biophys Acta. 1265(2-3):152-60.
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 Research Articles on DuSP3    1. DUSP3 is a pro-angiogenic atypical dual-specificity phosphatase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DuSP3 recombinant proteinPathways associated with DuSP3 recombinant protein
 Reference Product  PubMed Publications
 MAPK1 recombinant protein  >5 publications with DuSP3 and MAPK1
 ERBB2 recombinant protein  >2 publications with DuSP3 and ERBB2
 VRK3 recombinant protein  >2 publications with DuSP3 and VRK3
 MAPK3 recombinant protein  >2 publications with DuSP3 and MAPK3
 TYK2 recombinant protein  >1 publications with DuSP3 and TYK2
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 ERK/MAPK Targets Pathway antibodies  ERK/MAPK Targets Pathway Diagram
 ERKs Are Inactivated Pathway antibodies  ERKs Are Inactivated Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MAP Kinase Activation In TLR Cascade Pathway antibodies  MAP Kinase Activation In TLR Cascade Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Targets/ Nuclear Events Mediated By MAP Kinases Pathway antibodies  MAPK Targets/ Nuclear Events Mediated By MAP Kinases Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram
Diseases associated with DuSP3 recombinant proteinOrgans/Tissues associated with DuSP3 recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >7 publications with DuSP3 and Carcinoma
 Carcinoma, Non-Small-Cell Lung Antibodies  >3 publications with DuSP3 and Carcinoma, Non-Small-Cell Lung
 Carcinoma, Squamous Cell Antibodies  >2 publications with DuSP3 and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >2 publications with DuSP3 and Neoplasms, Experimental
 Uterine Cervical Neoplasms Antibodies  >2 publications with DuSP3 and Uterine Cervical Neoplasms
 Lung Neoplasms Antibodies  >2 publications with DuSP3 and Lung Neoplasms
 Nervous System Diseases Antibodies  >2 publications with DuSP3 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1 publications with DuSP3 and Neoplasm Metastasis
 Liver Diseases Antibodies  >1 publications with DuSP3 and Liver Diseases
 Inflammation Antibodies  >1 publications with DuSP3 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with DuSP3 and Blood
 Brain Antibodies  >4 publications with DuSP3 and Brain
 Cervix Antibodies  >3 publications with DuSP3 and Cervix
 Lung Antibodies  >3 publications with DuSP3 and Lung
 Embryonic Tissue Antibodies  >2 publications with DuSP3 and Embryonic Tissue
 Prostate Antibodies  >2 publications with DuSP3 and Prostate
 Nerve Antibodies  >2 publications with DuSP3 and Nerve
 Liver Antibodies  >1 publications with DuSP3 and Liver
 Eye Antibodies  >1 publications with DuSP3 and Eye
 Intestine Antibodies  >1 publications with DuSP3 and Intestine
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