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| Product Name
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E3 SUMO-protein ligase RanBP2 (RANBP2), partial, Recombinant Protein ★Popular Item★
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| Also Known As
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Recombinant Human E3 SUMO-protein ligase RanBP2 (RANBP2), partial
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| Product Synonym Names
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E3 SUMO-protein ligase RanBP2; EC=6.3.2.-; 358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270; Including the following 1 domains:; Putative peptidyl-prolyl cis-trans isomerase; PPIase; EC=5.2.
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| Product Gene Name
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[Similar Products]
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| Product Synonym Gene Name
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RANBP2; NUP358
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Sequence Positions
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2601-2802. Partial
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| Sequence
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PTEESSINYT FKTPEKAKEK KKPEDSPSDD DVLIVYELTP TAEQKALATK LKLPPTFFCY KNRPDYVSEE EEDDEDFETA VKKLNGKLYL DGSEKCRPLE ENTADNEKEC IIVWEKKPTV EEKAKADTLK LPPTFFCGVC SDTDEDNGNG EDFQSELQKV QEAQKSQTEE ITSTTDSVYT GGTEVMVPSF CKSEEPDSIT KS
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| OMIM
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gene 608033
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| 3D Structure
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ModBase 3D Structure for P49792
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| Host
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E Coli or Yeast or Baculovirus or Mammalian Cell
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| Purity/Purification
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>=90% (lot specific)
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| Form/Format
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Liquid containing glycerol
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| Tag Information
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This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information.
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| Sterility
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Sterile filter available upon request.
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| Endotoxin
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Low endotoxin available upon request.
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| Species
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Homo sapiens (Human)
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| Preparation and Storage
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Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Other Notes
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Small volumes of RANBP2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Binds single-stranded RNA (in vitro). May bind DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB.
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| SDS-PAGE of RANBP2 recombinant protein
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(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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TOP
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| NCBI/Uniprot data below describe general gene information for RANBP2. It may not necessarily be applicable to this product. |
| NCBI GI #
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150418007
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| NCBI GeneID
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5903
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| NCBI Accession #
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NP_006258.3
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_006267.4
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[Other Products]
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| UniProt Primary Accession #
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P49792
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[Other Products]
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| UniProt Secondary Accession #
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Q13074; Q15280; Q53TE2; Q59FH7
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[Other Products]
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| UniProt Related Accession #
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P49792
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[Other Products]
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| Molecular Weight
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358,199 Da
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TOP
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| NCBI Official Full Name
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E3 SUMO-protein ligase RanBP2
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| NCBI Official Synonym Full Names
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RAN binding protein 2
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| NCBI Official Symbol
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RANBP2
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[Similar Products]
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| NCBI Official Synonym Symbols
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ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358
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[Similar Products]
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| NCBI Protein Information
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E3 SUMO-protein ligase RanBP2; P270; nucleoporin 358; nucleoporin Nup358; 358 kDa nucleoporin; ran-binding protein 2; transformation-related protein 2; nuclear pore complex protein Nup358; acute necrotizing encephalopathy 1 (autosomal dominant)
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| UniProt Protein Name
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E3 SUMO-protein ligase RanBP2
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| UniProt Synonym Protein Names
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358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270
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| Protein Family
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E3 SUMO-protein ligase
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| UniProt Gene Name
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RANBP2
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[Similar Products]
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| UniProt Synonym Gene Names
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NUP358; RanBP2
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[Similar Products]
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| UniProt Entry Name
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RBP2_HUMAN
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| NCBI Summary for RANBP2
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RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008]
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| UniProt Comments for RANBP2
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RANBP2: a very large RAN-binding nucleoporin with E3 SUMO-protein ligase activity. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Forms a complex with SUMOylated RanGAP1 (the GTPase activating protein for Ran) that is required for efficient importin alpha/beta dependent nuclear import. May bind RNA or DNA. Component of the nuclear export pathway. Has a specific docking site for the nuclear export factor exportin-1. Contains a cyclophilin type peptidyl-prolyl cis-trans isomerase domain. A giant scaffolding protein that forms filaments extending from the nuclear pore into the cytoplasm. Regulates the localization of mitochondria in photosensory neurons. Deficits of RanBP2 downregulate glucose catabolism and energy homeostasis. Forms a tight complex with RANBP1 and UBE2I. Interacts with SUMO1 and PARK2. For some substrates, sumoylation and nuclear import are linked events. Sumoylates proteins including SP100 and HDAC4 deacetylase.
Protein type: EC 6.3.2.-; Ligase; Adaptor/scaffold; Nuclear export; EC 5.2.1.8; SUMO conjugating system; Nucleoporin
Chromosomal Location of Human Ortholog: 2q12.3
Cellular Component: nuclear membrane; mitochondrion; membrane; nuclear pore; cytosol; nuclear inclusion body
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; zinc ion binding; RNA binding; Ran GTPase binding; ligase activity
Biological Process: mRNA transport; protein peptidyl-prolyl isomerization; viral reproduction; protein folding; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; negative regulation of glucokinase activity; protein sumoylation; protein import into nucleus; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport
Disease: Encephalopathy, Acute, Infection-induced, Susceptibility To, 3
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TOP
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| Research Articles on RANBP2
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1. Case Report/Review: RANBP2-ALK gene rearrangement in inflammatory myofibroblastic tumors.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with RANBP2 recombinant protein | Pathways associated with RANBP2 recombinant protein |
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| Diseases associated with RANBP2 recombinant protein | Organs/Tissues associated with RANBP2 recombinant protein |
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TOP
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Recombinant Protein 
