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ERK1 recombinant protein

Scan QR to view Datasheet Catalog #    MBS515429
SDS-PAGE
Unit / Price
0.02 mg  /  $260 +1 FREE 8GB USB
0.05 mg  /  $415 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ERK1, Recombinant Protein

★Popular Item★
 Also Known As   

ERK1, Unactive recombinant protein

 Product Synonym Names    PRKM3; P44ERK1; P44MAPK; MAPK3
 Product Gene Name   

ERK1 recombinant protein

[Similar Products]
 Product Synonym Gene Name    MAPK3; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 16; NC_000016.9 (30125426..30134630, complement). Location: 16p11.2
 OMIM    601795
 3D Structure    ModBase 3D Structure for P27361
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 Purity/Purification    >80%
 Form/Format    Recomniant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
 Concentration    0.1 ug/ul (lot specific)
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 Preparation and Storage    Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.
 Other Notes    Small volumes of ERK1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ERK1 recombinant protein

   Recombinant full-length tag-free human ERK1 was expressed in E. coli cells.

Scientific Background: ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
 Product Categories/Family for ERK1 recombinant protein    Unactive Kinase; Signaling Proteins - Unactive Kinases
 Applications Tested/Suitable for ERK1 recombinant protein   

Kinase Assay, Western Blot (WB)

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 SDS-PAGE of ERK1 recombinant protein    The purity of ERK1 was determined to be >80% by densitometry. Approx. MW 44 kDa.
ERK1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ERK1. It may not necessarily be applicable to this product.
 NCBI GI #    91718898
 NCBI GeneID    5595
 NCBI Accession #    NP_002737.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002746 [Other Products]
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 UniProt Primary Accession #    P27361 [Other Products]
 UniProt Secondary Accession #    Q8NHX1; A8CZ58; B0LPG3 [Other Products]
 UniProt Related Accession #    P27361 [Other Products]
 Molecular Weight    ~44 kDa
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 NCBI Official Full Name    Homo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1, mRNA
 NCBI Official Synonym Full Names    mitogen-activated protein kinase 3
 NCBI Official Symbol    MAPK3 [Similar Products]
 NCBI Official Synonym Symbols   
ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
 UniProt Protein Name    Mitogen-activated protein kinase 3
 UniProt Synonym Protein Names   
ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1
 UniProt Gene Name    MAPK3 [Similar Products]
 UniProt Synonym Gene Names    ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK [Similar Products]
 UniProt Entry Name    MK03_HUMAN
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 NCBI Summary for ERK1    The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ERK1    Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Ref.11 Ref.13 Ref.14 Ref.15 Ref.18 Ref.19 Ref.22 Ref.24 Ref.25 Ref.26 Ref.28 Ref.36

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-202 and Tyr-204 in response to external stimuli like insulin or NGF. Both phosphorylations are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9. Ref.19 Ref.40

Subunit structure: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS1, MAP2K1/MEK1, MAPK3 and RGS14

By similarity. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2

By similarity. MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation

By similarity. Interacts with TPR. Ref.12 Ref.15 Ref.17 Ref.19 Ref.20 Ref.23 Ref.28 Ref.30 Ref.31 Ref.33 Ref.38 Ref.40

Subcellular location: Cytoplasm. Nucleus. Note: Autophosphorylation at Thr-207 promotes nuclear localization. Ref.17 Ref.40

Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Ref.17

Post-translational modification: Phosphorylated upon KIT and FLT3 signaling

By similarity. Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Ref.16 Ref.29 Ref.36 Ref.37 Ref.40 Ref.48

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.
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Product References and Citations for ERK1 recombinant protein

   1. Boulton, T G. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86.
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 Research Articles on ERK1    1. GLIPR-2 overexpression in HK-2 cells can potentiate EMT-like processes in this cell type through the ERK1/2 signaling pathway
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ERK1 recombinant proteinPathways associated with ERK1 recombinant protein
 Reference Product  PubMed Publications
 MAP2K1 recombinant protein  >355 publications with ERK1 and MAP2K1
 MAP2K2 recombinant protein  >147 publications with ERK1 and MAP2K2
 ELK1 recombinant protein  >103 publications with ERK1 and ELK1
 DUSP1 recombinant protein  >96 publications with ERK1 and DUSP1
 CREB1 recombinant protein  >33 publications with ERK1 and CREB1
 DUSP6 recombinant protein  >30 publications with ERK1 and DUSP6
 DUSP4 recombinant protein  >11 publications with ERK1 and DUSP4
 PTPN7 recombinant protein  >5 publications with ERK1 and PTPN7
 PTPRR recombinant protein  >2 publications with ERK1 and PTPRR
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
Diseases associated with ERK1 recombinant proteinOrgans/Tissues associated with ERK1 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2192 publications with ERK1 and Neoplasms
 Necrosis Antibodies  >734 publications with ERK1 and Necrosis
 Cardiovascular Diseases Antibodies  >679 publications with ERK1 and Cardiovascular Diseases
 Inflammation Antibodies  >661 publications with ERK1 and Inflammation
 Nervous System Diseases Antibodies  >496 publications with ERK1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >356 publications with ERK1 and Breast Neoplasms
 Heart Diseases Antibodies  >281 publications with ERK1 and Heart Diseases
 Hypertension Antibodies  >233 publications with ERK1 and Hypertension
 Hypertrophy Antibodies  >232 publications with ERK1 and Hypertrophy
 Liver Diseases Antibodies  >217 publications with ERK1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1610 publications with ERK1 and Vascular
 Blood Antibodies  >1524 publications with ERK1 and Blood
 Muscle Antibodies  >1442 publications with ERK1 and Muscle
 Brain Antibodies  >1134 publications with ERK1 and Brain
 Heart Antibodies  >644 publications with ERK1 and Heart
 Lung Antibodies  >567 publications with ERK1 and Lung
 Bone Antibodies  >483 publications with ERK1 and Bone
 Liver Antibodies  >482 publications with ERK1 and Liver
 Kidney Antibodies  >480 publications with ERK1 and Kidney
 Nerve Antibodies  >454 publications with ERK1 and Nerve
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