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EZCut TEV Protease Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS844472
Unit / Price
0.1 mg  /  $150 +1 FREE 8GB USB
1 mg  /  $415 +1 FREE 8GB USB
 
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 Product Name   

EZCut TEV Protease, Recombinant Protein

★Popular Item★
 Also Known As   

EZCut TEV Protease, Recombinant

 Product Synonym Names    Nuclear inclusion protein A; NIa protein
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Specificity    The EZCut TEV Protease has an activity of >=10,000 units/mg.
 Purity/Purification    >=95% by SDS-PAGE
 Form/Format    Liquid
1mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0
 Concentration    1 mg/ml (lot specific)
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 Unit Definition    One unit is defined as the amount of EZCut TEV Protease required to cleave >90% of 10 ug a control protein substrate (containing an ENLYFQS sequence at N-terminus) in 1 h at 37C.
    
 Source    E.coli
 Cleavage Protocol    In order to find the optimum cleavage conditions for a target fusion protein, it is recommended to run preliminary cleavage reactions at a small scale. Successful cleavage with TEV protease is dependent upon proper folding of the fusion protein that enables access of the TEV recognition sequence by the enzyme. The target fusion protein (1 mg/ml) containing TEV protease cleavage site should be purified to homogeneity. Recommended cleavage buffer is 50 mM Tris, buffer, 0.1 M NaCl, pH 8.0.
1.To 10 ul of target protein in an Eppendorf tube add 0.05-0.5 ug (0.5-5 U) of TEV protease.
2.Add 10 ul of freshly prepared 50 mM Tris, buffer, 0.1 M NaCl, pH 8.0 containing 5 mM DTT. Mix well by pipetting up and down; incubate at 37C for 1 h. Analyze by SDS-PAGE.
3.Once optimum cleavage conditions are obtained, the reaction can be scaled up to cleave the entire amount of the target fusion protein. After completion of cleavage reaction, TEV can be removed by passing the entire mixture through Ni-chelating resin. Collect the flow through and wash with the resin with 50 mM Tris, buffer, 0.1 M NaCl, pH 8.0. Combine the washes with the flow through to obtain cleaved protein product.
4. Optimum temperature for TEV protease cleavage is 34C. However, the reaction can also be carried out at 37C or 30C also. If the target protein is not stable at these temperatures, the cleavage reaction can also be performed at room temperature or 4C. In such cases, the amount of TEV protease should be optimized for different target proteins
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 Preparation and Storage    Store at -80C. Stable for at least 1 year as supplied. It may be further diluted to 0.1-0.5 mg/ml with 0.1M Tris-HCl, 0.5M NaCl, 20% glycerol, and stored at -80C in aliquots. Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of EZCut TEV Protease recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for EZCut TEV Protease recombinant protein

   Background: EZCut TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. EZCut TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
 Product Categories/Family for EZCut TEV Protease recombinant protein    Molecular Biology Tools; Molecular Biology Proteins/EnzymesProteins and Enzymes; ProteasesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)
 Applications Tested/Suitable for EZCut TEV Protease recombinant protein   

SDS-PAGE; HPLC

 Application Notes for EZCut TEV Protease recombinant protein    Recombinant EZCuTM TEV Protease can be used to cleave solubility, secretion, detection, and purification affinity tags from recombinant proteins containing TEV protease-specific cleavage site resulting in the target protein with only one extra amino acid at N-terminus in comparison to other endopeptidases. The extra amino acid (Glycine/Serine) is a smaller amino acid and may not cause significant changes to protein structure.
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 SDS-PAGE of EZCut TEV Protease recombinant protein    EZCut TEV Protease recombinant protein SDS-PAGE image
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 Testing Data of EZCut TEV Protease recombinant protein    EZCut TEV Protease recombinant protein Testing Data image
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 Molecular Weight    28.6 kDa (2038-2279 aa + C-terminal poly-his tag). [Similar Products]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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