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ENTPD1 recombinant protein :: Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS2018714 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
SDS-PAGE
Unit / Price
0.01 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $265 +1 FREE 8GB USB
0.1 mg  /  $405 +1 FREE 8GB USB
0.2 mg  /  $500 +1 FREE 8GB USB
0.5 mg  /  $970 +1 FREE 8GB USB
1 mg  /  $1,435 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1), Recombinant Protein

 Also Known As   

Recombinant Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1)

 Product Gene Name   

ENTPD1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TDE IGAYLAECME LSTELIPTSK HHQTPVYLGA TAGMRLLRME SEQSADEVLA AVSTSLKSYP FDFQGAKIIT GQEEGAYGWI TINYLLGRFT QEQSWLSLIS DSQKQETFGA LDLGGASTQI TFVPQNSTIE SPENSLQFRL YGEDYTVYTH SFLCYGKDQA LWQKLAKDIQ VSSGGVLKDP CFNPGYEKVV NVSELYGTPC TKRFEKKLPF DQFRIQGTGD YEQCHQSILE LFNNSHCPYS QCAFNGVFLP PL
 Chromosome Location    Chromosome: 19; NC_000085.6 (40612363..40741602). Location: 19 C3; 19 34.25 cM
 3D Structure    ModBase 3D Structure for P55772
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Mus musculus (Mouse)
 Expression System    Prokaryotic expression
 Residues    Thr98~Leu352 (Accession # P55772) with two N-terminal Tags, His-tag and T7-tag
 Subcellular Location    Membrane; Multi-pass membrane protein
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    5.5
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ENTPD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for ENTPD1 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable for ENTPD1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ENTPD1 recombinant protein    ENTPD1 recombinant protein SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ENTPD1. It may not necessarily be applicable to this product.
 NCBI GI #    6753346
 NCBI GeneID    12495
 NCBI Accession #    NP_033978.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_009848.3 [Other Products]
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 UniProt Primary Accession #    P55772 [Other Products]
 UniProt Related Accession #    P55772 [Other Products]
 Molecular Weight    32.3kDa
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 NCBI Official Full Name    ectonucleoside triphosphate diphosphohydrolase 1
 NCBI Official Synonym Full Names    ectonucleoside triphosphate diphosphohydrolase 1
 NCBI Official Symbol    Entpd1 [Similar Products]
 NCBI Official Synonym Symbols   
Cd39; AA408691; NTPDase-1; 2610206B08Rik
[Similar Products]
 NCBI Protein Information    ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; ectoapyrase; CD39 antigen; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen
 UniProt Protein Name    Ectonucleoside triphosphate diphosphohydrolase 1
 UniProt Synonym Protein Names   
Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen
 Protein Family    Ectonucleoside triphosphate diphosphohydrolase
 UniProt Gene Name    Entpd1 [Similar Products]
 UniProt Synonym Gene Names    Cd39; NTPDase 1; Ecto-ATPDase 1 [Similar Products]
 UniProt Entry Name    ENTP1_MOUSE
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 UniProt Comments for ENTPD1    ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.6.1.5; Membrane protein, multi-pass; Hydrolase; Nucleotide Metabolism - pyrimidine

Cellular Component: extracellular space; synaptic vesicle; neuron projection; cell surface; membrane; cell soma; basolateral plasma membrane; postsynaptic density; integral to membrane; basal lamina

Molecular Function: identical protein binding; hydrolase activity; nucleoside-diphosphatase activity; ATPase activity; nucleotide binding; nucleoside-triphosphatase activity; ATP binding; adenosine-diphosphatase activity

Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; metabolic process; purine ribonucleoside diphosphate catabolic process; negative regulation of dopamine secretion; protein homooligomerization
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 Research Articles on ENTPD1    1. Taken together, these data show that CD39 plays a prominent role in controlling ATP levels and thereby microglial phagocytosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ENTPD1 recombinant proteinDiseases associated with ENTPD1 recombinant protein
 Products by Pathway  Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with ENTPD1 and Inflammation
 Disease Models, Animal Antibodies  >8 publications with ENTPD1 and Disease Models, Animal
 Neoplasms Antibodies  >7 publications with ENTPD1 and Neoplasms
 Necrosis Antibodies  >5 publications with ENTPD1 and Necrosis
 Kidney Diseases Antibodies  >3 publications with ENTPD1 and Kidney Diseases
 Ischemia Antibodies  >3 publications with ENTPD1 and Ischemia
 Liver Diseases Antibodies  >3 publications with ENTPD1 and Liver Diseases
 Nervous System Diseases Antibodies  >2 publications with ENTPD1 and Nervous System Diseases
 Atherosclerosis Antibodies  >1 publications with ENTPD1 and Atherosclerosis
 Myocardial Infarction Antibodies  >1 publications with ENTPD1 and Myocardial Infarction
Organs/Tissues associated with ENTPD1 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with ENTPD1 and Blood
 Kidney Antibodies  >4 publications with ENTPD1 and Kidney
 Pancreas Antibodies  >3 publications with ENTPD1 and Pancreas
 Bone Marrow Antibodies  >3 publications with ENTPD1 and Bone Marrow
 Brain Antibodies  >2 publications with ENTPD1 and Brain
 Skin Antibodies  >2 publications with ENTPD1 and Skin
 Embryonic Tissue Antibodies  >2 publications with ENTPD1 and Embryonic Tissue
 Artery Antibodies  >2 publications with ENTPD1 and Artery
 Lung Antibodies  >1 publications with ENTPD1 and Lung
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