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ENPP1 recombinant protein :: Ectonucleotide Pyrophosphatase Recombinant Protein

Scan QR to view Datasheet Catalog #    MBS146019 ENPP1 recombinant protein
Unit / Price
0.002 mg  /  $140 +1 FREE 8GB USB
0.01 mg  /  $205 +1 FREE 8GB USB
1 mg  /  $5,015 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ectonucleotide Pyrophosphatase (ENPP1), Recombinant Protein

★Popular Item★
 Also Known As   

Recombinant Human Ectonucleotide Pyrophosphatase

 Product Synonym Names    Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, ENPP1, M6S1, NPPS, PC1, PDNP1, NPP1, PC-1, PCA1, ARHR2, COLED
 Product Gene Name   

ENPP1 recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    ASKPSCAKEV KSCKGRCFER TFGNCRCDAA CVELGNCCLD YQETCIEPEH IWTCNKFRCG EKRLTRSLCA CSDDCKDKGD CCINYSSVCQ GEKSWVEEPC ESINEPQCPA GFETPPTLLF SLDGFRAEYL HTWGGLLPVI SKLKKCGTYT KNMRPVYPTK TFPNHYSIVT GLYPESHGII DNKMYDPKMN ASFSLKSKEK FNPEWYKGEP IWVTAKYQGL KSGTFFWPGS DVEINGIFPD IYKMYNGSVP FEERILAVLQ WLQLPKDERP HFYTLYLEEP DSSGHSYGPV SSEVIKALQR VDGMVGMLMD GLKELNLHRC LNLILISDHG MEQGSCKKYI YLNKYLGDVK NIKVIYGPAA RLRPSDVPDK YYSFNYEGIA RNLSCREPNQ HFKPYLKHFL PKRLHFAKSD RIEPLTFYLD PQWQLALNPS ERKYCGSGFH GSDNVFSNMQ ALFVGYGPGF KHGIEADTFE NIEVYNLMCD LLNLTPAPNN GTHGSLNHLL KNPVYTPKHP KEVHPLVQCP FTRNPRDNLG CSCNPSILPI EDFQTQFNLT VAEEKIIKHE TLPYGRPRVL QKENTICLLS QHQFMSGYSQ DILMPLWTSY TVDRNDSFST EDFSNCLYQD FRIPLSPVHK CSFYKNNTKV SYGFLSPPQL NKNSSGIYSE ALLTTNIVPM YQSFQVIWRY FHDTLLRKYA EERNGVNVVS GPVFDFDYDG RCDSLENLRQ KRRVIRNQEI LIPTHFFIVL TSCKDTSQTP LHCENLDTLA FILPHRTDNS ESCVHGKHDS SWVEELLMLH RARITDVEHI TGLSFYQQRK EPVSDILKLK THLPTFSQED GPKLHHHHHH .
 OMIM    125853
 3D Structure    ModBase 3D Structure for P22413
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 Purity/Purification    Greater than 95% as determined by SDS-PAGE
 Form/Format    Filtered (0.4 um) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Filtered lyophilized (freeze-dried) powder.
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 Source    HEK 293
 Solubility    It is recommended to add 200ul deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
    
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 Preparation and Storage    Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time.
 Other Notes    Small volumes of ENPP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for ENPP1 recombinant protein

   Description: ENPP1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 98-925) containing a total of 840 amino acids, having a molecular mass of 96.5kDa (calculated) though it migrates at approximately 110kDa on SDS PAGE, the ENPP1 is also composed of a 2 a.a N-terminal linker, a 4 a.a C-terminal linker and fused to a 6 a.a His tag at C-Terminus.The Human ENPP1 is purified by proprietary chromatographic techniques.

Introduction: Ectonucleotide Pyrophosphatase (ENPP1) belongs to the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. ENPP1 is a type II transmembrane glycoprotein comprised of 2 identical disulfide-bonded subunits. The ENPP1 protein has broad specificity and cleaves various substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. The ENPP1 protein can hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and it may also hydrolyze diadenosine polyphosphates. ENPP1 gene mutations are linked with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance.
 Product Categories/Family for ENPP1 recombinant protein    ENZYMES; Enzymes; Phosphatase
NCBI/Uniprot data below describe general gene information for ENPP1. It may not necessarily be applicable to this product.
 NCBI GI #    170650661
 NCBI GeneID    5167
 NCBI Accession #    NP_006199.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006208.2 [Other Products]
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 UniProt Primary Accession #    P22413 [Other Products]
 UniProt Secondary Accession #    Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3 [Other Products]
 UniProt Related Accession #    P22413 [Other Products]
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 NCBI Official Full Name    ectonucleotide pyrophosphatase/phosphodiesterase family member 1
 NCBI Official Synonym Full Names    ectonucleotide pyrophosphatase/phosphodiesterase 1
 NCBI Official Symbol    ENPP1 [Similar Products]
 NCBI Official Synonym Symbols   
M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
[Similar Products]
 NCBI Protein Information    ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1
 UniProt Protein Name    Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
 UniProt Synonym Protein Names   
Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1Including the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase
 Protein Family    Ectonucleotide pyrophosphatase/phosphodiesterase
 UniProt Gene Name    ENPP1 [Similar Products]
 UniProt Synonym Gene Names    M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase [Similar Products]
 UniProt Entry Name    ENPP1_HUMAN
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 NCBI Summary for ENPP1    This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ENPP1    ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; EC 3.6.1.9; Phosphodiesterase; Nucleotide Metabolism - purine; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.1.4.1

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; basolateral plasma membrane; lysosomal membrane; integral to membrane; plasma membrane

Molecular Function: phosphodiesterase I activity; protein binding; nucleotide diphosphatase activity; protein homodimerization activity; nucleic acid binding; 3'-phosphoadenosine 5'-phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; insulin receptor binding; ATP binding; polysaccharide binding

Biological Process: receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; inorganic diphosphate transport; water-soluble vitamin metabolic process; negative regulation of glycogen biosynthetic process

Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on ENPP1    1. findings show the Q allele of the ENPP1 K121Q gene may contribute to the susceptibility for type 2 diabetes in Caucasians and Asians
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ENPP1 recombinant proteinPathways associated with ENPP1 recombinant protein
 Reference Product  PubMed Publications
 NT5E recombinant protein  >5 publications with ENPP1 and NT5E
 CD38 recombinant protein  >1 publications with ENPP1 and CD38
 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram
Diseases associated with ENPP1 recombinant proteinOrgans/Tissues associated with ENPP1 recombinant protein
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >64 publications with ENPP1 and Diabetes Mellitus, Type 2
 Obesity Antibodies  >55 publications with ENPP1 and Obesity
 Arterial calcification of infancy Antibodies  >32 publications with ENPP1 and Arterial calcification of infancy
 Kidney Diseases Antibodies  >23 publications with ENPP1 and Kidney Diseases
 Congenital Abnormalities Antibodies  >16 publications with ENPP1 and Congenital Abnormalities
 Disease Models, Animal Antibodies  >16 publications with ENPP1 and Disease Models, Animal
 Hypertension Antibodies  >11 publications with ENPP1 and Hypertension
 Liver Diseases Antibodies  >9 publications with ENPP1 and Liver Diseases
 Inflammation Antibodies  >6 publications with ENPP1 and Inflammation
 Hypophosphatemic Rickets, Autosomal Recessive, 2 Antibodies  >5 publications with ENPP1 and Hypophosphatemic Rickets, Autosomal Recessive, 2
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >57 publications with ENPP1 and Bone
 Blood Antibodies  >51 publications with ENPP1 and Blood
 Heart Antibodies  >25 publications with ENPP1 and Heart
 Muscle Antibodies  >22 publications with ENPP1 and Muscle
 Skin Antibodies  >21 publications with ENPP1 and Skin
 Kidney Antibodies  >20 publications with ENPP1 and Kidney
 Liver Antibodies  >18 publications with ENPP1 and Liver
 Connective Tissue Antibodies  >16 publications with ENPP1 and Connective Tissue
 Embryonic Tissue Antibodies  >7 publications with ENPP1 and Embryonic Tissue
 Pancreas Antibodies  >4 publications with ENPP1 and Pancreas
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