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FHIT recombinant protein

Scan QR to view Datasheet Catalog #    MBS203463
SDS-Page
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
0.5 mg  /  $640 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FHIT, Recombinant Protein

★Popular Item★
 Also Known As   

FHIT, 1-147aa, Human, His tag, E Coli

 Product Synonym Names    Fragile histidine triad protein; Bis (5'-adenosyl)-triphosphatase; AP3Aase; Fragile histidine triad protein AP3A hydrolase; AP3A hydrolase fragile site 3p14.2; Bis 5' adenosyl triphosphatase; Diadenosine 5' 5''' P1 P3 triphosphate hydrolase; Dinucleosidetriphosphatase; FRA 3B; FRA3B; Fragile histidine triad; Fragile histidine triad gene; Fragile histidine triad protein; Tumor suppressor protein.
 Product Gene Name   

FHIT recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH
 OMIM    U46922 mRNA
 3D Structure    ModBase 3D Structure for P49789
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    Human
 Tag Information    His-tag
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of FHIT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for FHIT recombinant protein

   FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography.
 Product Categories/Family for FHIT recombinant protein    Enzymes & Proteases
 Applications Tested/Suitable for FHIT recombinant protein   

SDS-PAGE

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 SDS-Page of FHIT recombinant protein    FHIT recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for FHIT. It may not necessarily be applicable to this product.
 NCBI GI #    4503719
 NCBI GeneID    2272
 NCBI Accession #    NP_002003 [Other Products]
 NCBI GenBank Nucleotide #    NM_002012.3 [Other Products]
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 UniProt Primary Accession #    P49789 [Other Products]
 UniProt Secondary Accession #    Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9 [Other Products]
 UniProt Related Accession #    P49789 [Other Products]
 Molecular Weight    17.9 kDa (155aa), confirmed by MALDI-TOF
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 NCBI Official Full Name    bis(5'-adenosyl)-triphosphatase isoform 1
 NCBI Official Synonym Full Names    fragile histidine triad
 NCBI Official Symbol    FHIT [Similar Products]
 NCBI Official Synonym Symbols   
FRA3B; AP3Aase
[Similar Products]
 NCBI Protein Information    bis(5'-adenosyl)-triphosphatase
 UniProt Protein Name    Bis(5'-adenosyl)-triphosphatase
 UniProt Synonym Protein Names   
AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein
 Protein Family    Bis(5'-adenosyl)-triphosphatase
 UniProt Gene Name    FHIT [Similar Products]
 UniProt Synonym Gene Names    AP3Aase [Similar Products]
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 NCBI Summary for FHIT    The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]
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 UniProt Comments for FHIT    Cleaves P1-P3-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P1-P4-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P1-P3-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
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Product References and Citations for FHIT recombinant protein

   Kmiec Z., et al. (2009) J Physiol Pharmacol. 60 (4):63-70.; Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136 (3):333-50.;
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 Research Articles on FHIT    1. It has been proposed that Fhit and Wwox loss work synergistically in cancer progression and that DNA damage caused by Fhit could be targeted early in cancer initiation for prevention, while DNA damage caused by Wwox loss could be targeted later in cancer progression, particularly in cancers that develop resistance to genotoxic therapies. (Review)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FHIT recombinant proteinPathways associated with FHIT recombinant protein
 Reference Product  PubMed Publications
 CDKN2A recombinant protein  >142 publications with FHIT and CDKN2A
 RASSF1 recombinant protein  >42 publications with FHIT and RASSF1
 HINT1 recombinant protein  >10 publications with FHIT and HINT1
 SRC recombinant protein  >9 publications with FHIT and SRC
 CCND1 recombinant protein  >8 publications with FHIT and CCND1
 APTX recombinant protein  >1 publications with FHIT and APTX
 Products by Pathway  Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
Diseases associated with FHIT recombinant proteinOrgans/Tissues associated with FHIT recombinant protein
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >572 publications with FHIT and Carcinoma
 Lung Neoplasms Antibodies  >207 publications with FHIT and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >188 publications with FHIT and Carcinoma, Squamous Cell
 Lung Diseases Antibodies  >178 publications with FHIT and Lung Diseases
 Carcinoma, Non-Small-Cell Lung Antibodies  >103 publications with FHIT and Carcinoma, Non-Small-Cell Lung
 Breast Neoplasms Antibodies  >103 publications with FHIT and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >95 publications with FHIT and Neoplasm Metastasis
 Uterine Cervical Neoplasms Antibodies  >78 publications with FHIT and Uterine Cervical Neoplasms
 Stomach Neoplasms Antibodies  >75 publications with FHIT and Stomach Neoplasms
 Neoplasms, Experimental Antibodies  >72 publications with FHIT and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >254 publications with FHIT and Lung
 Blood Antibodies  >87 publications with FHIT and Blood
 Stomach Antibodies  >80 publications with FHIT and Stomach
 Lymph Node Antibodies  >76 publications with FHIT and Lymph Node
 Kidney Antibodies  >69 publications with FHIT and Kidney
 Liver Antibodies  >54 publications with FHIT and Liver
 Brain Antibodies  >24 publications with FHIT and Brain
 Skin Antibodies  >21 publications with FHIT and Skin
 Intestine Antibodies  >19 publications with FHIT and Intestine
 Muscle Antibodies  >16 publications with FHIT and Muscle
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