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G6PD recombinant protein

Scan QR to view Datasheet Catalog #    MBS203264
SDS-Page
Unit / Price
0.1 mg  /  $255 +1 FREE 8GB USB
0.5 mg  /  $535 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

G6PD, Recombinant Protein

★Popular Item★
 Also Known As   

G6PD,1-491aa, E Coli, E Coli (Bioactivity Validated)

 Product Synonym Names    Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; zwf; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.
 Product Gene Name   

G6PD recombinant protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
 3D Structure    ModBase 3D Structure for P0AC53
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 Host    E Coli
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 Purity/Purification    > 95% by SDS-PAGE
 Form/Format    In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol
 Concentration    1 mg/ml (determined by Bradford assay) (lot specific)
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 Antigen Species    E Coli
 Biological Activity    Specific activity is > 70 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
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 Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of G6PD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for G6PD recombinant protein

   Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E Coli and purified by conventional chromatography techniques.
 Product Categories/Family for G6PD recombinant protein    Enzymes & Proteases
 Applications Tested/Suitable for G6PD recombinant protein   

SDS-PAGE

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 SDS-Page of G6PD recombinant protein    G6PD recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.
 NCBI GI #    16129805
 NCBI GeneID    946370
 NCBI Accession #    NP_416366 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.3 [Other Products]
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 UniProt Primary Accession #    P0AC53 [Other Products]
 UniProt Secondary Accession #    P22992; P78069; Q60134; Q60139 [Other Products]
 Molecular Weight    55.7kDa (491 aa), confirmed by MALDI-TOF.
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 NCBI Official Full Name    glucose-6-phosphate 1-dehydrogenase
 NCBI Official Symbol    zwf [Similar Products]
 NCBI Official Synonym Symbols   
ECK1853; JW1841
[Similar Products]
 NCBI Protein Information    glucose-6-phosphate 1-dehydrogenase
 UniProt Protein Name    Glucose-6-phosphate 1-dehydrogenase
 UniProt Gene Name    zwf [Similar Products]
 UniProt Synonym Gene Names    EDF [Similar Products]
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 NCBI Summary for G6PD    Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].
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 UniProt Comments for G6PD    Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.
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Product References and Citations for G6PD recombinant protein

   Huang Y., et al. (2008) Mol Genet Metab. 93 (1):44-53.; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5):1327-33;
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 Research Articles on G6PD    1. G6PDH and FPR could act concertedly to deliver reducing equivalents from carbohydrates, via NADP(+), to the FPR acceptors ferredoxin and/or flavodoxin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with G6PD recombinant proteinPathways associated with G6PD recombinant protein
 Reference Product  PubMed Publications
 gnd recombinant protein  >41 publications with G6PD and gnd
 pgi recombinant protein  >34 publications with G6PD and pgi
 edd recombinant protein  >25 publications with G6PD and edd
 eda recombinant protein  >22 publications with G6PD and eda
 pgl recombinant protein  >9 publications with G6PD and pgl
 gapA recombinant protein  >2 publications with G6PD and gapA
 nagE recombinant protein  >1 publications with G6PD and nagE
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 Entner-Doudoroff Pathway, Glucose-6P => Glyceraldehyde-3P + Pyruvate antibodies  Entner-Doudoroff Pathway, Glucose-6P => Glyceraldehyde-3P + Pyruvate Diagram
 Entner-Doudoroff Pathway, Glucose-6P => Glyceraldehyde-3P + Pyruvate antibodies  Entner-Doudoroff Pathway, Glucose-6P => Glyceraldehyde-3P + Pyruvate Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Microbial Metabolism In Diverse Environments Pathway antibodies  Microbial Metabolism In Diverse Environments Pathway Diagram
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